Evaluation of the Cytotoxic, Antioxidative and Antimicrobial Effects of Dracocephalum moldavica L. Cultivars

Simea, Ștefania; Ielciu, Irina; Hanganu, Daniela; Niculae, Mihaela; Páll, Emöke; Burtescu, Ramona Flavia; Oláh, Neli-Kinga; Cenariu, Mihai; Oniga, Ilioara; Benedec, Daniela; Duda, Marcel

doi:10.3390/molecules28041604

Cited by 4 publications

(3 citation statements)

References 61 publications

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“…1. Prior to in vivo administration, all of the ethanol was removed by using a rotary evaporator (Hahnvapor, HS-2000NS, Hahnshin Scientific Co., Sejong, Republic of Korea) at a temperature of 40 • C, and, subsequently, the sample was reconstituted with distilled water and immediately administered to animals [30][31][32][33].…”

Section: Extraction Procedures and Sample Preparationmentioning

confidence: 99%

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Betula pendula Leaf Extract Targets the Interplay between Brain Oxidative Stress, Inflammation, and NFkB Pathways in Amyloid Aβ1-42-Treated Rats

Sevastre-Berghian,

Ielciu,

Bab

et al. 2023

Antioxidants

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Alzheimer’s disease (AD) is known as the primary and most common cause of dementia in the middle-aged and elderly population worldwide. Chemical analyses of B. pendula leaf extract (BPE), performed using spectrophotometric and chromatographic methods (LC/MS), revealed high amounts of polyphenol carboxylic acids (gallic, chlorogenic, caffeic, trans-p-coumaric, ferulic, and salicylic acids), as well as flavonoids (apigenin, luteolin, luteolin-7-O-glucoside, naringenin, hyperoside, quercetin, and quercitrin). Four groups of Wistar rats were used in this experiment (n = 7/group): control (untreated), Aβ1-42 (2 μg/rat intracerebroventricular (i.c.v.), Aβ1-42 + BPE (200 mg/Kg b.w.), and DMSO (10 μL/rat). On the first day, one dose of Aβ1-42 was intracerebroventricularly administered to animals in groups 2 and 3. Subsequently, BPE was orally administered for the next 15 days to group 3. On the 16th day, behavioral tests were performed. Biomarkers of brain oxidative stress Malondialdehyde (MDA), (Peroxidase (PRx), Catalase (CAT), and Superoxid dismutase (SOD) and inflammation (cytokines: tumor necrosis factor -α (TNF-α), Interleukin 1β (IL-1β), and cyclooxygenase-2 (COX 2)) in plasma and hippocampus homogenates were assessed. Various protein expressions (Phospho-Tau (Ser404) (pTau Ser 404), Phospho-Tau (Ser396) (pTau Ser 396), synaptophysin, and the Nuclear factor kappa B (NFkB) signaling pathway) were analyzed using Western blot and immunohistochemistry in the hippocampus. The results show that BPE diminished lipid peroxidation and neuroinflammation, modulated specific protein expression, enhanced the antioxidant capacity, and improved spontaneous alternation behavior, suggesting that it has beneficial effects in AD.

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Section: Extraction Procedures and Sample Preparationmentioning

confidence: 99%

“…Nitrogen at 35 psi and 10 L/min was used as a drying gas. The capillary potential was set to +3000 V [31][32][33].…”

Section: Lc/ms Analysismentioning

confidence: 99%

Betula pendula Leaf Extract Targets the Interplay between Brain Oxidative Stress, Inflammation, and NFkB Pathways in Amyloid Aβ1-42-Treated Rats

Sevastre-Berghian,

Ielciu,

Bab

et al. 2023

Antioxidants

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“…The UPLC-Q-TOF-MS method was used to study the qualitative and quantitative composition of secondary metabolites (flavonoids, phenolic acids, and coumarins) in the aerial part of D. moldavica, depending on the growth period and geographical location [11]. The study of polyphenolic compounds in D. moldavica using LC-MS revealed the content of rosmarinic acid as the main component, in the range of 5.337 ± 0.0411 and 6.320 ± 0.0535 mg/mL [12].…”

Section: Introductionmentioning

confidence: 99%

Dracocephalum jacutense Peschkova from Yakutia: Extraction and Mass Spectrometric Characterization of 128 Chemical Compounds

et al. 2023

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Dracocephalum jacutense Peschkova is a rare and endangered species of the genus Dracocephalum of the Lamiaceae family. The species was first described in 1997 and listed in the Red Data Book of Yakutia. Significant differences in the multicomponent composition of extracts from D. jacutense collected in the natural environment and successfully introduced in the Botanical Garden of Yakutsk were identified by a team of authors earlier in a large study. In this work, we studied the chemical composition of the leaves, stem, and inflorescences of D. jacutense using the tandem mass spectrometry method. Only three cenopopulations of D. jacutense were found by us in the territory of the early habitat—in the vicinity of the village of Sangar, Kobyaysky district of Yakutia. The aboveground phytomass of the plant was collected, processed and dried as separate parts of the plant: inflorescences, stem and leaves. Firstly, a total of 128 compounds, 70% of which are polyphenols, were tentatively identified in extracts of D. jacutense. These polyphenol compounds were classified as 32 flavones, 12 flavonols, 6 flavan-3-ols, 7 flavanones, 17 phenolic acids, 2 lignans, 1 dihydrochalcone, 4 coumarins, and 8 anthocyanidins. Other chemical groups were presented as carotenoids, omega-3-fatty acids, omega-5-fatty acids, amino acids, purines, alkaloids, and sterols. The inflorescences are the richest in polyphenols (73 polyphenolic compounds were identified), while 33 and 22 polyphenols were found in the leaves and stems, respectively. A high level of identity for polyphenolic compounds in different parts of the plant is noted for flavanones (80%), followed by flavonols (25%), phenolic acids (15%), and flavones (13%). Furthermore, 78 compounds were identified for the first time in representatives of the genus Dracocephalum, including 50 polyphenolic compounds and 28 compounds of other chemical groups. The obtained results testify to the unique composition of polyphenolic compounds in different parts of D. jacutense.

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Rosmarinic acid content and antioxidant capacity in Dracocephalum moldavica hairy roots affected by iron and copper nanoparticles

Khanlari,

Kiarostami,

Namin

et al. 2024

Preprint

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The present study aimed to develop a protocol for hairy root induction from Dracocephalum moldavica leaf explants and evaluate the effects of iron nanoparticles (Fe NPs) (0, 250, 500 and 1000 ppm), copper nanoparticles (Cu NPs) (0, 25, 50 and 100 ppm) and the combination of both (Fe/Cu NPs) on antioxidant capacity, phenolic, flavonoids and rosmarinic acid content of hairy roots. Hairy root induction in D. moldavica was significantly (P ≤ 0.01) influenced by Agrobacterium rhizogenes strains, culture media and sucrose concentration. The highest hairy root induction (86/6%) was related to ATCC15834 strain on Murashige and Skoog culture medium supplemented with 30 g l− 1 sucrose. The individual and combined effect of Fe and Cu NPs on the measured traits was highly significant (P ≤ 0.01). The highest amount of rosmarinic acid (26.38 mg/g DW) was obtained in Cu NPs (100 ppm) with Fe NPs (1000 ppm) treatment that was 6.6-fold higher compared to control. The highest amount of flavonoids, malondialdehyde (MDA), phenolic compounds, hydrogen peroxide (H2O2), proline, and anthocyanin production was obtained in Cu NPs (100 ppm) + Fe NPs (1000 ppm) treatment that was 1.86, 1.56, 1.76, 2.07, 1.62 and 1.65-fold higher compared to respective control. Correlation and principal component analysis revealed a clear separation between treatments groups and control also a positive correlation among rosmarinic acid production and other traits. The findings of this study indicated the best results were obtained with the combined effect of Cu NPs (100 ppm) with Fe NPs (1000 ppm) treatment.

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Evaluation of the Cytotoxic, Antioxidative and Antimicrobial Effects of Dracocephalum moldavica L. Cultivars

Cited by 4 publications

References 61 publications

Betula pendula Leaf Extract Targets the Interplay between Brain Oxidative Stress, Inflammation, and NFkB Pathways in Amyloid Aβ1-42-Treated Rats

Betula pendula Leaf Extract Targets the Interplay between Brain Oxidative Stress, Inflammation, and NFkB Pathways in Amyloid Aβ1-42-Treated Rats

Dracocephalum jacutense Peschkova from Yakutia: Extraction and Mass Spectrometric Characterization of 128 Chemical Compounds

Rosmarinic acid content and antioxidant capacity in Dracocephalum moldavica hairy roots affected by iron and copper nanoparticles

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