Evaluation of the cytotoxic effects of a new Harvard MTA compared to MTA Flow and ProRoot MTA on human gingival fibroblasts

Youssef, Abdel‐Rahman; Elsherief, Samia

doi:10.1016/j.sdentj.2020.04.009

Cited by 8 publications

(15 citation statements)

References 42 publications

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“…When comparing MTT methodology with others, previous studies have demonstrated that MTT prevent over or underestimation of cell viability during cytotoxicity evaluation, thus considered reliable. [34] As well as, MTT has also been used in previous studies to determine cytotoxic effects caused by different dental materials on HGF, [35] human periodontal ligament fibroblast cells, [36] and in mouse fibroblast cells. [37] Likewise, previous studies have evaluated cell viability of TiO 2 N using MTT method on human cells.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of fluoride release in glass ionomer cements modified with titanium dioxide nanoparticles

et al. 2022

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Background: Several efforts have been made to improve the glass ionomer cements (GICs) properties with nanotechnology. Fluoride release in once of most beneficial properties of GICs. The purpose of this study was to evaluate the fluoride release, recharge, and cytotoxicity in GICs reinforced with titanium dioxide nanoparticles (TiO 2 N).Objective: Evaluate the fluoride release, recharge, and cytotoxicity in GICs reinforced with TiO 2 N.Methods: Four GICs, FUJI IX EXTRA (G1c), KETAC MOLAR (G2c), IONOFILL MOLAR (G3c), and FUJI IX (G4c) were combined with TiO 2 N (G1e, G2e, G3e, and G4e) and divided into blocks of 5-mm width and 1-mm thickness 10 each. A total of 80 samples were arranged as follows: GICs alone as negative control (n = 40) and GICs + TiO 2 N as experimental groups (n = 40). The fluoride release was determined for periods of 1, 2, 6, 10, 31, 90, 180, 240, and 300 days. On days 30 and 179, samples were recharged by submerging in 1 mL of 20,000 ppm sodium fluoride gel. Cytotoxic activity was carried out with gingival fibroblasts, using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide cell viability assay.Results: The experimental groups obtained the highest and more constant fluoride released when compared to control groups. After the first recharge, experimental groups (G1e, G3e, and G4e) showed statistically significant results (P = .001, 0.010, and 0.001 respectively) enhancing their recharge ability regarding control groups. The second recharge showed better results in G1e concerning the rest of the groups. No cytotoxic activity was observed in all experimental groups, although significant differences were observed in G3e and G4e regarding control group. Conclusion:The incorporation of TiO 2 N enhance the fluoride release in glass ionomers with a noncytotoxic effect on human gingival fibroblasts.Abbreviations: GICs = glass ionomer cements, G1c = Glass ionomer FUJI IX EXTRA, G2c = Glass ionomer KETAC MOLAR, G3c = Glass ionomer IONOFILL MOLAR, G4c = Glass ionomer IONOFILL MOLAR, G1e = Glass ionomer FUJI IX EXTRA modified with titanium dioxide nanoparticles, G2e = Glass ionomer KETAC MOLAR modified with titanium dioxide nanoparticles, G3e = Glass ionomer IONOFILL MOLAR modified with titanium dioxide nanoparticles, G4e = Glass ionomer IONOFILL MOLAR modified with titanium dioxide nanoparticles,HGF = human gingival fibroblasts, PDL = population doubled level, TiO 2 = titanium dioxide, TISAB = total ionic strength adjustment buffer solution, TiO 2 N = titanium dioxide nanoparticles.

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Section: Discussionmentioning

confidence: 99%

Enhancement of fluoride release in glass ionomer cements modified with titanium dioxide nanoparticles

et al. 2022

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“…Two formulations of MTA eluates were used in this study, MTA with 4-and 48-hours setting times respectively. The 4 hours setting time is the primary setting time based on the manufacturer's recommendation, while MTA with 48 hours setting time is the complete setting time as proposed by other studies in the literature [16,23].…”

Section: Discussionmentioning

confidence: 99%

“…MTA was prepared as eluates, where the leachable content from the set material is collected in the culture media. This will re ect the actual scenario intra-orally as the material was under constant challenge by saliva and dentinal uids [22,23]. Two formulations of MTA eluates were used in this study, MTA with 4-and 48-hours setting times respectively.…”

Section: Discussionmentioning

confidence: 99%

Preliminary in vitro cytotoxic evaluation of Uncaria gambier (Hunt) Roxb extract as a potential herbal-based pulpotomy medicament

Tan

Mahyuddin

Sockalingam

et al. 2022

Preprint

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Background The downfall of formocresol as a pulpotomy medicament highlights the importance of cytotoxic evaluation and the establishment of a safe concentration of dental material prior to its usage in the oral cavity. Uncaria gambir is a herbal plant that possesses antimicrobial, anti-inflammatory and antioxidant properties, suggesting its potential as an alternative medicament for pulpotomy. However, a study on its cytotoxicity is lacking. Here, we investigated the concentration and time-dependent toxicity of Uncaria gambir extract towards the M3CT3-E1 cell line and compared it with the gold standard pulpotomy medicament: mineral trioxide aggregate (MTA). Methods Uncaria gambir extracts at concentrations ranging from 1000 to 7.8 µg/ml and MTA eluates at 4- and 48 hours setting times were prepared. 10% dimethyl sulfoxide (DMSO) and culture media were used as positive and negative control respectively. Cell viability on days 1, 2, 3 and 7 were analysed using Alamar Blue and Live and Dead Cell assay. Any morphological cellular changes were evaluated using transmission electron microscopes (TEM). Data were analysed using a two-way mixed Analysis of Variance (ANOVA). Results The interaction between the concentration and exposure time on the fluorescence intensity of Uncaria gambir extract and MTA 48 hours was found to be statistically significant (p < 0.001). No cytotoxic effects on the cells were exerted by both MTA 48 hours and Uncaria gambir extract at a concentration below 500 µg/mL. TEM analysis and Live and Dead Cell assay for both materials were comparable to the negative control. No significant differences in fluorescent intensity were observed between Uncaria gambir extract at 500 µg/mL and MTA 48 hours (p > 0.05). Conclusion Uncaria gambir extracts at a maximum concentration of 500 µg/mL are non-cytotoxic over time and are comparable to the MTA.

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“…Some important previous studies have also demonstrated good responses to MTA Flow (Bueno et al, 2019;Mondelli et al, 2019;Youssef & Elsherief, 2021). The cited studies obtained their results based on the evaluation of human gingival fibroblasts (HGF) by the MTT assay (Youssef & Elsherief, 2021), by histological analysis associated with the measurement of fibrous capsule thickness using Masson's trichrome-stained sections (Mondelli et al, 2019), or by histological analysis associated with the evaluation of mineralization by Von Kossa staining (Bueno et al, 2019).…”

Section: Daysmentioning

confidence: 99%

“…This material is composed of small‐sized particles (<10 μm) that form a paste of smooth consistency and easy application within a short setting time (<15 min). Moreover, MTA Flow has distinct alkalinizing activity, low solubility, high radiopacity and the capacity to form a layer of hydroxyapatite when set, which are relevant properties for the promotion of tissue healing (Guimarães et al, 2017; Youssef & Elsherief, 2021). Previous in vitro studies of the different physicochemical, antimicrobial and biological properties of MTA Flow showed that it is a suitable reparative endodontic cement with similar behaviour when compared to other materials (Guimarães et al, 2017; Pelepenko et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity, biocompatibility and osteoinductive profile of an MTA‐hydrogel‐based cement: An in vitro and animal study

et al. 2023

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Aim This study aimed to evaluate the cytotoxicity, biocompatibility and osteoinductive profile of a mineral trioxide aggregate (MTA)‐hydrogel‐based material (MTA Flow) in comparison with MTA Angelus. Methodology Cell viability was evaluated in human periodontal ligament stem cells (hPDLSCs) using the methyl‐thiazol‐tetrazolium (MTT) colourimetric assay. Polyethylene tubes containing the tested materials and empty polyethylene tubes (control) were implanted in the subcutaneous tissue of Wistar rats. Cellular (lymphocyte infiltration) and extracellular events (ECM; collagen fibres) were analysed in histological sections. Immunohistochemical (collagen I, osteopontin, bone sialoprotein, bone morphogenetic protein4) analyses were also performed. Results At 24, 48 and 72 h, all tested groups showed cell viability similar to control (p > .05). Regarding biocompatibility, all groups showed similar cellular events represented by a slight inflammatory reaction characterized by hyperaemia and a mild lymphocytic inflammatory infiltrate. The analysis of lymphocytes during the time showed a decrease in these cells in the control group and a significant interaction between MTA Angelus and control (p < .001), with MTA Angelus showing a more extensive inflammatory infiltrate. Regarding fibres, an increase in content was observed in all groups during the experimental time (7, 30 and 60 days), however, no difference was detected among the experimental groups (p = .063). After 60 days, the immunoexpression of bone matrix proteins in the MTA Flow group was similar to or higher than that observed in the MTA Angelus and in the control group. Conclusions MTA Flow showed a non‐cytotoxic behaviour, biocompatibility and ability to stimulate tissue mineralization.

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Evaluation of the cytotoxic effects of a new Harvard MTA compared to MTA Flow and ProRoot MTA on human gingival fibroblasts

Cited by 8 publications

References 42 publications

Enhancement of fluoride release in glass ionomer cements modified with titanium dioxide nanoparticles

Enhancement of fluoride release in glass ionomer cements modified with titanium dioxide nanoparticles

Preliminary in vitro cytotoxic evaluation of Uncaria gambier (Hunt) Roxb extract as a potential herbal-based pulpotomy medicament

Cytotoxicity, biocompatibility and osteoinductive profile of an MTA‐hydrogel‐based cement: An in vitro and animal study

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