Evaluation of the discriminatory power of pulsed-field gel electrophoresis and PCR fingerprinting for epidemiologic typing of Candida species

Voß, Andreas; Pfaller, Michael A.; Hollis, Richard J.; Melchers, Willem J. G.; Meis, Jacques F.

doi:10.1111/j.1469-0691.1998.tb00360.x

Cited by 10 publications

(4 citation statements)

References 47 publications

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“…On only 5 (4.1%) of 123 occasions were both the healthcare worker and the patient for whom care was provided found to carry the same strain of yeast. This finding, obtained using two different typing methods [40], supports prior studies showing possible cross-transmission of yeast strains from healthcare workers to patients [11,15,28,36], but it does not prove that crosstransmission occurred. Further studies focusing on daily healthcare worker-patient interactions, taking into account such factors as hand washing, glove use, and use of invasive devices, should help define further the role that healthcare worker-patient interactions play in the transmission of yeasts.…”

Section: Discussionsupporting

confidence: 77%

Epidemiology of Yeast Colonization in the Intensive Care Unit

Hedderwick¹,

Lyons²,

Liu³

et al. 2000

European Journal of Clinical Microbiology & Infectious Dise

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In order to investigate the epidemiology of colonization and possible transmission of yeasts among patients and healthcare workers in adult intensive care units (ICUs), 194 patients were followed for a mean of 9 +/- 11 days and 63 healthcare workers were followed for a mean of 132 +/- 52 days. Among the patients, 142 (73%) were colonized by yeast, with Candida albicans being the species most commonly recovered. Most patients (65%) were already colonized with yeast upon admission to the intensive care unit; only 17% became colonized after admission. Persistent colonization occurred in 51 (55%) of 92 patients who had more than three cultures performed; in 75% of them, colonization persisted with the same strain of Candida albicans or Candida glabrata. Bacterial infection in the month preceding entry into the ICU was the only risk factor significantly associated with yeast colonization. Among the healthcare workers, yeasts were isolated from 42 (67%). Candida albicans was most frequently recovered from the oropharynx (19% of occasions), and Candida parapsilosis was most frequently found on hands (8% of occasions). Persistent colonization of the oropharynx occurred in only six healthcare workers, and none had persistence of yeasts on hands. In this non-outbreak setting, 5 (4%) of 123 patient/healthcare worker interactions that were linked epidemiologically yielded the same strain of Candida albicans, providing evidence for possible cross-transmission. No similar link was found between healthcare worker-patient interactions and colonization with Candida glabrata or Candida parapsilosis.

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Section: Discussionsupporting

confidence: 77%

Epidemiology of Yeast Colonization in the Intensive Care Unit

Hedderwick¹,

Lyons²,

Liu³

et al. 2000

European Journal of Clinical Microbiology & Infectious Dise

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“…Applied to specific situations, each of these methods has advantages and disadvantages. Karyotyping by PFGE was shown to be a useful molecular typing tool for various Candida species [18]. In our study electrophoretic karyotyping using PFGE showed a single chromosomal pattern for these outbreak isolates.…”

Section: Discussionsupporting

confidence: 54%

Outbreak of nosocomial fungemia caused by Candida glabrata

Koç

Kocagöz

Erdem

et al. 2002

Mycoses

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An outbreak of Candida glabrata fungemia that was thought to be associated with bottles used for milk feeds occurred at our children's infectious diseases clinic. This cluster of cases was investigated using a case-control study. Isolates were identified by conventional methods and karyotyped using pulsed-field gel electrophoresis (PFGE) of genomic DNA. Potential risk factors for nine hospitalized children with candidemia and 14 controls were long-term hospitalization and treatments with more than two antibiotics. Electrophoretic karyotyping showed a single chromosomal pattern for these outbreak isolates and, in addition, they all had the same antifungal susceptibility results. These findings suggest that clonal dissemination of a single strain was responsible for this outbreak. Karyotyping by PFGE appears to be a useful molecular typing method for strains of C. glabrata.

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“…The unweighted pair group method using arithmetic averages (UPGMA) was used for cluster analysis. Isolates were considered different when the band similarity value was less than 95 % (Voss et al, 1998).…”

Section: Methodsmentioning

confidence: 99%

Comparison of four molecular typing methods to assess genetic relatedness of Candida albicans clinical isolates in Taiwan

Chen

Lin

et al. 2005

Journal of Medical Microbiology

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This report describes the investigation of the genetic profiles of 53 Candida albicans isolates collected from 18 hospitals in Taiwan using three PFGE-based typing methods (PFGE karyotyping, and PFGE of Sfi I and BssHII restriction fragments) and one repetitive-sequence-PCR (rep-PCR) method. All four methods were able to identify clonal related isolates from the same patients. PFGEBssHII exhibited the highest discriminatory power by discriminating 40 genotypes, followed by PFGE-Sfi I (35 genotypes) and then by rep-PCR (31 genotypes), while PFGE karyotyping exhibited the lowest discriminatory power (19 genotypes). High discriminatory power can also be achieved by combining typing methods with different typing mechanisms, such as rep-PCR and PFGE-based typing methods. The results also showed that the genotype of each isolate was patient-specific and not associated with the source of the isolation, geographic origin or antifungal resistance.

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Evaluation of the discriminatory power of pulsed-field gel electrophoresis and PCR fingerprinting for epidemiologic typing of Candida species

Cited by 10 publications

References 47 publications

Epidemiology of Yeast Colonization in the Intensive Care Unit

Epidemiology of Yeast Colonization in the Intensive Care Unit

Outbreak of nosocomial fungemia caused by Candida glabrata

Comparison of four molecular typing methods to assess genetic relatedness of Candida albicans clinical isolates in Taiwan

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