Evaluation of the DNS method for analysing lignocellulosic hydrolysates

Marsden, Warwick L.; Gray, Peter P.; Nippard, Greg J.; Quinlan, Mark

doi:10.1002/jctb.5030320744

Cited by 87 publications

(32 citation statements)

References 10 publications

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“…Dinitrosalicylic acid is reduced to red aminonitrosalicylic acid by reducing sugars. The red colour produced was spectrophotometrically measured at 510 nm (Marsden et al 1982). The amount of sugar was calculated by comparison with a standard.…”

Section: Estimation Of Sugarmentioning

confidence: 99%

Traditional fermentation of Ayurvedic medicine yields higher proinflammatory enzyme inhibition compared to wine-model product

Chandra

Preethidan

Sabu

et al. 2015

Frontiers in Life Science

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This study investigated the process of preparing fermented medicines as prescribed in Ayurveda, the traditional Indian system of medicine. Berberine, an alkaloid, was used as a model compound. Berberine is the active constituent of Berberis aristata and is alleged to have anti-inflammatory effect. Biotransformation of berberine was studied by the phospholipase A 2 assay in fermented products prepared in traditional and commercially available brewer's yeast-induced environments. Sugar and alcohol levels were estimated to indicate the culmination of fermentation. It was confirmed that traditional fermentation biotransforms berberine to a greater extent than commercially available brewer's yeast-induced fermentation. Therefore, fermentation induced by commercially available yeast is no substitute for the ethnopharmacological and traditional fermentation prescribed in the traditional Indian system of medicine.

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Section: Estimation Of Sugarmentioning

confidence: 99%

Traditional fermentation of Ayurvedic medicine yields higher proinflammatory enzyme inhibition compared to wine-model product

Chandra

Preethidan

Sabu

et al. 2015

Frontiers in Life Science

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“…1 filter paper. The filtrates were analyzed for reducing sugar hydrolyzed from cellulose using the 3,5-dinitrosalicylic acid (DNS) method (Marsden et al 1982). The levels of reducing sugar were converted to the weight of cellulose per weight of the dried sample using standard glucose solutions.…”

Section: Measurement Of Residual Cellulosementioning

confidence: 99%

“…Cellulose and hemicellulose are the major contributors for SRB growth by supplying carbon source in the form of organic acid (Marsden et al 1982). As a VFP operates, cellulose and hemicellulose would gradually decompose to low molecular weight organics to feed cellulolytic microbes as well as sulfatereducing bacteria.…”

Section: Introductionmentioning

confidence: 99%

Longevity of organic layers of vertical flow ponds for sulfate reduction in treating mine drainages in South Korea

Cheong

Hur

Yim

et al. 2011

Environ Geochem Health

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This study was carried out to evaluate longevity of available organic materials used for sulfate-reducing bacteria (SRB) activity in vertical flow ponds (VFPs) to treat mine drainage in South Korea. Spent mushroom compost samples (SMC) were tested as substrates in VFPs and analyzed for total organic carbon in VFPs, and were collected to analyze total organic carbon (TOC), T-N, T-P, K, metals and residual cellulose to check the longevity assessment. Chemical analysis revealed that the average contents of Fe, Al and Mn in SMC of VFPs were 19,907, 32,137 and 434 mg/kg, respectively. The contents of Fe and Al in SMC of VFPs were much higher than those of the unused SMC (control), but to the contrary, those of Mn showed a reversed tendency. Average TOC content of the controls was 64.19% but in one of the VFP substrates was as low as 15.92%. This might be resulted from SRB consumed the available organic carbon in SMC as VFPs system aged. Contents of T-N in VFPs tended to decrease as VFPs aged. The residual cellulose ranged from 3.88 to 6.72% (g/g). There existed a negative relationship between residual cellulose contents and ages of VFPs. Assuming that SMC in all VFPs had similar compositions when the VFPs were initially established, trend analysis predicted that the amount of carbon source for SRB might be available for 12-15 years further, depending on VFPs.

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“…El contenido de azúcares reductores en las muestras hidrolizadas se determinó utilizando el método del ácido 3,5-dinitrosalicílico (DNS) con glucosa como estándar (Marsden et al, 1982;Miller, 1959). La determinación del contenido de etanol en los caldos de fermentación se llevó a cabo mediante la técnica SPME en un espectrómetro CG-FID empleando la columna de alta resolución HP Innowax 19091N-233.…”

Section: Caracterización De Las Materias Primas Y De Los Materiales Lunclassified

Efecto del Pretratamiento de Biomasa Maderera en el Rendimiento a Etanol

Gómez

Ríos

Peña³

2013

Inf. tecnol.

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Evaluation of the DNS method for analysing lignocellulosic hydrolysates

Cited by 87 publications

References 10 publications

Traditional fermentation of Ayurvedic medicine yields higher proinflammatory enzyme inhibition compared to wine-model product

Traditional fermentation of Ayurvedic medicine yields higher proinflammatory enzyme inhibition compared to wine-model product

Longevity of organic layers of vertical flow ponds for sulfate reduction in treating mine drainages in South Korea

Efecto del Pretratamiento de Biomasa Maderera en el Rendimiento a Etanol

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