Evaluation of the effect of nitrate and chloride on Cd(ii)-induced cell oxidative stress by scanning electrochemical microscopy

Abstract

Cadmium (Cd) is one of the most prevalent toxic metal pollutants, which is widely distributed in various environmental medium and organisms. Literatures have documented that Cd could stimulate cellular oxidative...

Study on inhibitory effects of AsA, ZnCl2, and BAPTA-AM on Cd2+-induced cell oxidative stress and cytotoxicity by scanning electrochemical microscopy (SECM) technology

“…Previously, we studied the effect of CdCl 2 on MCF-7 breast cancer cell oxidative stress. 40 At a working potential of −0.65 V, SECM was used to detect ROS release from MCF-7 cells incubated with different concentrations of CdCl 2 for 2 h. Under the stimulation of CdCl 2 , the release of ROS from MCF-7 cells was concentration- and time-dependent. Following continued elevation in Cd 2+ exposure concentration and time, the cells would undergo significant oxidative damage, impairing the antioxidant defense system.…”

“…As a membrane-permeable Ca 2+ chelator, BAPTA-AM could break the intracellular calcium homeostasis and inhibit Ca 2+ -mediated potassium ion channels, resulting in abnormal ion exchange inside and outside the cell. 40 Briefly, at low incubation concentrations (≤10 μmol L −1 ), BAPTA-AM had low cytotoxicity to cells, and the redox state in cells was stable. Therefore, the physiological function of cells was normal.…”