Evaluation of the Effect of Pinus nigra L. and Pinus brutia L. (Pine) Alcoholic Bark Extracts on Production Potential of Virulence Factors About Anti-QS Activity Against Pseudomonas aeruginosa and Chromobacterium violaceum

İbrahim, Bashar

doi:10.22312/sdusbed.1129144

Cited by 1 publication

(1 citation statement)

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“…The elastolytic activity of the extract was determined according to the method of Fuentes [30]. The inhibitory effect of extracted oils on elastase production was investigated by the Elastin Congo Red (ECR) test.…”

Section: Performing the Elastolytic Testmentioning

confidence: 99%

An Evaluation of the Anti-QS Activity and Virulence Factors Production Potential of Rhamnus cathartica L. against Some Gram-Positive and Gram-Negative Bacteria

IBRAHIM,

REİS,

ARIN

et al. 2023

Preprint

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The study aims to evaluate the Quorum Sensing (QS) system inhibition against some Gram-positive and Gram-negative bacteria detected by molecular modeling of R. cathartica L. plant extract. R. cathartica L. methanol extract was evaluated in vitro. The research was carried out for the possible role and mechanism of R. cathartica L. The antibacterial activity of R. cathartica L. seed extract on some Gram-positive and Gram-negative strains was evaluated using culture-based and computational analysis. Gentamicin (CN; 30 µg/ml, Bioanalyse) was used as an antibacterial control of the extract on the production potential of QS-dependent virulence factors on Pseudomonas aeruginosa PAO1 was investigated. Phytochemical analysis resulted in the identification of 23 phenolic phytochemicals. R. cathartica L. extract was observed to have antimicrobial activity against Gram-positive and Gram-negative bacteria at different rates (11.3 mm-16 mm). The inhibitory effect of R. cathartica L. extract on P. aeruginosa PAO1 (elastase 61%, pyocyanin 18%, and biofilm 61%) was recorded moderately. Phytochemical analysis revealed that the main component of the extract is kaempferol. Therefore, the binding potential of kaempferol on QS system receptors was investigated via molecular docking. Computational analysis showed that kaempferol can inhibit the QS system by preventing competitive ligands from connecting to LasR. This study explored the activity and mechanism of action for a new green alternative against resistance. might provide a mechanism for R. cathartica L. seed extract components and thus provide a new avenue for using R. cathartica L. seed extract as a green antibacterial agent.

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Section: Performing the Elastolytic Testmentioning

confidence: 99%