Evaluation of the effect of static magnetic fields combined with human hepatocyte growth factor on human satellite cell cultures

Birk, Richard; Sommer, Ulrich; Faber, Anne; Aderhold, Christoph; Schulz, Johannes D.; Hörmann, K.; Goessler, Ulrich Reinhart; Stern‐Straeter, Jens

doi:10.3892/mmr.2014.2083

Cited by 5 publications

(16 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Enriched biological processes and molecular functions were related to iron ion binding and oxygen transport in Cluster 4, which was identified as the erythrocyte population. Cluster 7 was identified as ACTA1 group with high expression of ACTA1, which was one of marker genes for human stem cells [ 18 ]. Cluster 9 was uncertain because of the lack of a known marker for gene expression or a known enriched pathway.…”

Section: Resultsmentioning

confidence: 99%

Identification of diverse cell populations in skeletal muscles and biomarkers for intramuscular fat of chicken by single-cell RNA sequencing

Xing

Zhao

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

Background The development of skeletal muscle is closely related to the efficiency of meat production and meat quality. Chicken skeletal muscle development depends on myogenesis and adipogenesis and occurs in two phases—hyperplasia and hypertrophy. However, cell profiles corresponding to the two-phase muscle development have yet to be determined. Single-cell RNA-sequencing (scRNA-seq) can elucidate the cell subpopulations in tissue and capture the gene expression of individual cells, which can provide new insights into the myogenesis and intramuscular adipogenesis. Results Ten cell clusters at the post-hatching developmental stage at Day 5 and seven cell clusters at the late developmental stage at Day 100 were identified in chicken breast muscles by scRNA-seq. Five myocyte-related clusters and two adipocyte clusters were identified at Day 5, and one myocyte cluster and one adipocyte cluster were identified at Day 100. The pattern of cell clustering varied between the two stages. The cell clusters showed clear boundaries at the terminal differentiation stage at Day 100; by contrast, cell differentiation was not complete at Day 5. APOA1 and COL1A1 were selected from up-regulated genes in the adipocyte cluster and found to be co-expressed with the ADIPOQ adipocyte marker gene in breast muscles by RNA in situ hybridization. Conclusions This study is the first to describe the heterogeneity of chicken skeletal muscle at two developmental stages. The genes APOA1 and COL1A1 were identified as biomarkers for chicken intramuscular fat cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Identification of diverse cell populations in skeletal muscles and biomarkers for intramuscular fat of chicken by single-cell RNA sequencing

Xing

Zhao

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…同时有多项研究表明稳态磁场并不影响细胞内的中间纤维. 例如, 稳态磁场并不影响人成肌/间叶干细胞内肌间线蛋白 (desmin)的基因表达 [62] , 并且肝细胞生长因子(HGF) 以及肝细胞生长因子与稳态磁场联合都不影响人卫星细胞内肌间线蛋白的基因表达 [63] .…”

Section: 稳态磁场与微丝unclassified

“…例如 , 1964年, Nakai [64] 发现外加的稳态磁场(强度未知)可以增强角蛋白(keratin)合成的电子显微镜放射自显影, 但机制不清. 80 mT稳态磁场可以增强胰岛素样生长因子 1(IGF-1) 诱导的肌间线蛋白基因表达升高 [65] . 另外, 还有多项实验结果表明低频交变磁场 (属于非稳态磁场)可以影响人牙槽骨间充质干细胞中波形蛋白(vimentin)的基因表达 [66] , 但是并不影响人乳腺癌细胞中的角蛋白 [67] .…”

Section: 然而也有一些结果表明一些细胞内的中间纤unclassified

Effects of static magnetic fields on eukaryotic cytoskeleton

Zhang¹,

Li²,

Zhang³

2019

Chin. Sci. Bull.

View full text Add to dashboard Cite

“…Myoblasts cultivated in growth medium (GM) under stimulation of SMF showed a higher fusion index, indicative of a higher degree of differentiation, compared to myoblast cell cultures exposed to additional stimulation with a differentiation medium (DM). Likewise, the additional stimulation of human myoblasts with SMFs und hepatocyte growth factor (HGF) did not result in the assumed increase in myogenic differentiation (12). While an increase in marker gene expression in human myoblast cultures under SMF and by adding insulin-like growth factor (IGF) was detected, no contractile skeletal muscle was found (13).…”

Section: Introductionmentioning

confidence: 98%

“…Likewise, the additional stimulation of human myoblasts with SMFs und hepatocyte growth factor (HGF) did not result in the assumed increase in myogenic differentiation (12). While an increase in marker gene expression in human myoblast cultures under SMF and by adding insulin-like growth factor (IGF) was detected, no contractile skeletal muscle was found (13).…”

Section: Introductionmentioning

confidence: 98%

Influence of static magnetic fields on human myoblast/mesenchymal stem cell co‑cultures

et al. 2017

Self Cite

View full text Add to dashboard Cite

The results of surgical repair of extensive muscle tissue defects are still of primary concern, leaving patients with residual cosmetic and functional impairments. Therefore, skeletal muscle tissue engineering attempts to grow functional neo‑tissue from human stem cells to promote tissue regeneration and support defect closure. Despite intensive research efforts, the goal of stable induction of myogenic differentiation in expanded human stem cells by using clinically feasible stimuli, has not yet been reached to a sufficient extent. Therefore, the present study investigated the differentiation potential of static magnetic fields (SMFs), using co‑cultures of human satellite cells and human mesenchymal stem cells (MSCs). It has previously been demonstrated that SMFs may act as a promising myogenic stimulus. Tests were performed on co‑cultures with and without SMF exposure, using growth medium [high growth factor concentrations (GM)] and differentiation medium [low growth factors concentrations (DM)]. AlamarBlue® assay‑based cell proliferation analysis revealed no significant difference between co‑cultures with, vs. without SMF stimulation, regardless of growth factor concentrations in the cell culture medium. To determine the degree of differentiation in co‑cultures under stimulation with SMFs, semi‑quantitative gene expression measurements of the following marker genes were performed: Desmin, myogenic factor 5, myogenic differentiation antigen 1, myogenin, adult myosin heavy chain 1 and skeletal muscle α1 actin. In neither GM nor DM was a steady, significant increase in marker gene expression detected. Verifying the gene expression findings, immunohistochemical antibody staining against differentiation markers revealed that SMF exposure did not enhance myogenic maturation. Therefore, SMF treatment of human satellite cell/MSC co‑cultures did not result in the desired increase in myogenic differentiation. Further studies are required to identify a suitable stimulus for skeletal muscle tissue engineering.

show abstract

Evaluation of the effect of static magnetic fields combined with human hepatocyte growth factor on human satellite cell cultures

Cited by 5 publications

References 23 publications

Identification of diverse cell populations in skeletal muscles and biomarkers for intramuscular fat of chicken by single-cell RNA sequencing

Identification of diverse cell populations in skeletal muscles and biomarkers for intramuscular fat of chicken by single-cell RNA sequencing

Effects of static magnetic fields on eukaryotic cytoskeleton

Influence of static magnetic fields on human myoblast/mesenchymal stem cell co‑cultures

Contact Info

Product

Resources

About