Evaluation of the Effect of Five Colombian Propolis Extracts on the Expression of Genes Associated with Cell Cycle and Inflammation in a Canine Osteosarcoma Cell Line

Abstract

Introduction: Propolis has anti-inflammatory, antitumor, antibacterial and immunomodulatory properties, which is why it is suggested that it could be used as an alternative or complementary drug therapy in the treatment of various pathologies such as cancer, diseases chronic-degenerative and infectious. Materials and Methods: In this study, canine bone fibroblasts were used as control cells, and the canine Osteosarcoma cell line (OSCA-8) was acquired to evaluate the effects of ethanol extracts of propolis from five Colombian regions on these cells. The cytotoxic effect was evaluated by examining changes in cell viability and proliferation. Furthermore, the expression of some relevant and characteristic genes related to the tumor phenotype, related to the proinflammatory process and cell cycle, was assessed. Results: Thus, the evaluation of the relative expression of some genes associated with the cell cycle and inflammation could improve the understanding of the cytotoxic effect of propolis extracts on OSCA-8 cell lines. Conclusion:The first time in Colombia, the biological activity of ethanol extracts of propolis was evaluated regarding the inflammation and cell cycle pathways. After 48 hr, the Colombian EEP had an effect on the increase in both OSCA-8 cells and fibroblasts.

Physicochemical Characterization, Antioxidant, and Proliferative Activity of Colombian Propolis Extracts: A Comparative Study

“…In comparison with Colombian propolis from the Cauca and Cundinamarca region, our results differ from those reported by Torres et al [72], where these extracts presented cytotoxic effects and, in turn, induced retention of the cell cycle in the G0/G1 phase in canine fibroblasts. This may be associated with cellular sensitivity or a chemical composition rich in benzophenes and diterpenes unlike the propolis of the Huila, evaluated in this study, which demonstrates the great variety and different effects that this natural product can present.…”