Evaluation Of the Effect of New Generation Quinolone Antibiotics and Immunepotent CRP on The Immune System

Abstract

Human peripheral blood mononuclear cells (PBMCs) are affected by new generation quinolone antibiotics (Ciprofloxacin & Ofloxacin) as well as Immunepotent CRP (IMP-CRP) when it comes to lymphocyte populations and immune mediator production. The objective of this work is to evaluate the effect of quinolones, Ciprofloxacin and Ofloxacin on the immune system, focusing on the mechanisms of oxidative activity of monocytes, lymphocyte populations and their production of cytokines; contributing to the body of knowledge of these antibiotics; since they are the most used and considered as the new generation. On the other hand, the action of IMP-CRP is evaluated, which has antibacterial and anti-inflammatory activity; on these same parameters, which had not previously been considered. After treating the individuals' blood with quinolones and Immune potent CRP and its various combinations, peripheral blood mononuclear cells were separated by density gradient from the peripheral blood of healthy people. NBT was used to detect monocyte oxidative activity, flow cytometry was used to measure the percentage of B, NK and selected T cells, and ELISA was used to measure the production of the cytokines IL-2, IL-10, and IFN-γ. MTT was used to measure relative cell viability. The results revealed that the medications did not influence the ability of the PBMCs to live. There was no noticeable variation in the oxidative activity of monocytes between treatments and controls, nor was there a discernible difference in the percentages of any of the cell populations studied. IL-2 production was increased in Ciprofloxacin and ofloxacin treatments (p<0.02) but unaffected in Immune potent CRP and combination therapies. Treatments with Ciprofloxacin, ofloxacin, and Immune potent CRP were observed to reduce IL-10 production (p<0.046). Treatments with Ciprofloxacin had no effect on IFN-γ production; however, treatments with ofloxacin (50 g/mL), Immune potent CRP, and their combinations significantly reduced IFN-γ production (p 0.01). Despite the fact that Ciprofloxacin, ofloxacin, and Immune potent CRP did not affect PBMCs viability, phagocyte activity, or lymphocyte populations, these antibiotics were able to alter cytokine production of IL-2, IL-10, and IFN-γ.