Evaluation of the effect of daptomycin, a glycopeptide agent, on intact intervertebral disc tissue

Abstract

AIM: To evaluate the effects of pre-and intra-operatively administered daptomycin (DAP) on the intact human primary intervertebral disc tissue cells. MATERIAL and METHODS: Primary cell cultures were established using tissues obtained through decompressive laminectomy, traumatic intervertebral disc herniation excision, and posterior transpedicular stabilization. Non-drug-administered samples were used as a control group. The samples treated with DAP formed the study group. Molecular assays for proliferation and gene expression were performed. The obtained data were evaluated statistically, and results with a value of p<0.05 were accepted as significant. RESULTS: While no reduction was observed in the proliferation, the gene expression of intact intervertebral disc tissue cells was time-dependently decreased compared to the control group, and these results were reported to be statistically significant. CONCLUSION: This study observed the effect that a pharmaceutical preparation, which was used on intervertebral disc tissue before and after the operation, had on normal, healthy, and intact tissue. It concludes that alterations in the expression of genes involved in the anabolic and/or catabolic process, even in adjacent healthy tissue, may slow down the healing process of the damaged tissue or cause undesired cell differentiation.

Toxicity of the acetyl‑para‑aminophenol group of medicines to intact intervertebral disc tissue cells

“…Therefore, the results obtained from assays using animal tissues may diverge from those using human tissues, which may result in misleading outcomes (6)(7)(8)(9)(10)(11)13,14). Commercial cell lines are known to comprise only a single type of cell, and there are no complicated coordination mechanisms in the microenvironment of the cells (6)(7)(8)(9)(10)(11)13,14). They do not have the same genotypic and/or phenotypic characteristics as in the human body; therefore, the results of studies using cell lines may also be misleading (6)(7)(8)(9)(10)(11)13,14).…”

“…Microphotographs of cell structures were obtained ( Fig. 1; second lane), and the images were evaluated using the CytoVision ® v 7.0 (DM6000B; Leica Microsystems GmbH) capture station imaging program (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16).…”

“…A notable number of studies have been performed to identify an appropriate treatment procedure for damaged osseous joints, facet joints and intervertebral disc structures, and investigations on the toxicity of drugs in non-degenerative, healthy tissues has gained momentum (10,11).…”