Evaluation of the Effect of Pentoxifylline on Cisplatin-Induced Testicular Toxicity in Rats

Abstract

Chemotherapy is associated with male infertility. Cisplatin (cis-diamminedichloro-platinum (II) (CDDP) as a chemotherapy medication used to treat a number of cancers has been reported to most likely induce testicular toxicity. Administration of antioxidants, such as pentoxifylline (PTX) may reduce some Adverse Drug Reactions (ADRs) of CDDP. Therefore, this study investigated the potentially protective effects of PTX on CDDP-induced testicular toxicity in adult male rats. For this purpose, 42 male rats were randomly divided into 7 groups. The rats were orally pretreated with PTX at the 3 doses of 75, 150, and 300 mg/kg once a day for 14 successive days. On the 14th day of the study, they were intraperitoneally (IP) administered with a single dose of CDDP (7 mg/kg). Finally, the sperm/testis parameters, serum levels of reproductive hormones, including testosterone, Luteinizing Hormone (LH), and Follicle Stimulating Hormone (FSH) as the pivotal endocrine factors controlling testicular functions, and histopathological changes of testis tissue were examined. Pretreatment with the two doses of 75 and 150 mg/kg PTX indicated significant increases in the sperm count and motility induced by CDDP administration. The right and significantly left testis weights were decreased following the treatment with 300 mg/kg of PTX plus CDDP. However, 75 mg/kg of PTX plus CDDP showed the best near-to-normal histopathological features. The results demonstrated that PTX alone enhanced some parameters, such as the sperm count, while reducing other parameters, including sperm fast motility and germ layer thickness. Furthermore, despite testosterone or LH levels, the mean serum FSH level was significantly augmented by the doses of 75 and 150 mg/kg. It was concluded that PTX administration cannot reduce CDDP-induced testicular toxicity even at high doses (e.g., 300 mg/kg), while it seemed to partially intensify CDDP toxicity effects at a dose of 75 mg/kg. Thus, further research is required in this regard.

The Protective Effect of Grape Seed and Skin Extract and Ulva rigida Against Oxidative Stress Induced by Cisplatin on The Testis of Rats

“…Cisplatin significantly increased testis O2°- (Fig 2A), MDA ( Fig 2B) and carbonylated proteins ( Fig 2C). Cisplatin has been described to induce testicular toxicity with stimulation of the production of free radical [15]. This has been confirmed in our study where we have detected in cisplatin-treated rats increased production of anion superoxide in the testicles.…”

The antiapoptotic and antioxidant effects of eugenol against cisplatin‐induced testicular damage in the experimental model

“…It is important to use agents having various biological effects such as antioxidant properties in various organ toxicity models experimentally formed with chemotherapeutic drugs. Until today in numerous researches, it used various agents to reduce or mitigate cisplatin‐induced testis toxicity (Fallahzadeh et al, ; Reddy et al, ). This study was presented to explain the potential ameliorative role of eugenol in reducing of cisplatin‐induced testis toxicity on count and morphology of spermatozoa in rats.…”

Beneficial role of virgin coconut oil supplementation against acute methotrexate chemotherapy-induced oxidative toxicity and inflammation in rats

“…Several studies have implicated increased generation of reactive oxygen and nitrogen species and the consequent impairment in antioxidant defense systems in drug-induced toxicity. 32 Oxidative stress and lipid peroxidation has emerged to play a critical role in the pathogenesis of MTX-induced toxicity. Interestingly, the single administration of MTX (20 mg/kg) to rats induced prominent alterations in antioxidant defense mechanisms.…”