Evaluation of the Effect of Different Doses of Low Energy Shock Wave Therapy on the Erectile Function of Streptozotocin (STZ)-Induced Diabetic Rats

Liu, Jing; Zhou, Feng; Li, Guangyong; Wang, Lin; Li, Huixi; Bai, Guangwei; Guan, Ruili; Xu, Yongde; Gao, Ze-Zhu; Tian, Wenjie; Xin, Zhongcheng

doi:10.3390/ijms140510661

Cited by 84 publications

(76 citation statements)

References 32 publications

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“…In rats with prolonged diabetes, and thus exposed to more pronounced effects of DM, the positive recovery effect of ESWT may reach statistical significance. This notion is supported by Liu et al [20] study in which expression levels of VEGF significantly changed in diabetic rats which received ESWT treatment twelve weeks after the STZ injection. [20] Ito et al [21] reported the effectiveness of L-ESWT treatment on cardiovascular diseases via promotion of angiogenesis through upregulation of the expression levels of related molecules levels including VEGF.…”

Section: Discussionsupporting

confidence: 76%

“…This notion is supported by Liu et al [20] study in which expression levels of VEGF significantly changed in diabetic rats which received ESWT treatment twelve weeks after the STZ injection. [20] Ito et al [21] reported the effectiveness of L-ESWT treatment on cardiovascular diseases via promotion of angiogenesis through upregulation of the expression levels of related molecules levels including VEGF. After the demonstration of microcirculation augmenting effect L-ESWT, the center of interest has gradually shifted from shock waves to L-ESWT.…”

Section: Discussionsupporting

confidence: 76%

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Effect of low-energy shockwave therapy on angiogenic factors in the penile tissue of diabetic rats

Ortaç

Küçükergin

Salabaş

et al. 2017

Turkish Journal of Urology

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Objective: The aim of this study is to investigate the effect of low-energy shock wave therapy (LESWT) on angiogenesis factors at penile tissue in a diabetic rat model. Material and methods:A total of 30 male Sprague-Dawley rats which were allocated into three equal groups were included study. Group 1 (control group) included 10 male rats which did not receive any treatment were randomly chosen to serve as normal control. The remaining rats were injected intraperitoneally with 60 mg/kg of streptozotocin (STZ) to induce diabetes. Diabetic rats were divided into two equal group which constituted diabetic control, and LESWT treatment (DM+LESWT) group. Each rat in the DM+LESWT group received L-ESWT therapy. Endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) mRNA expression levels of penile tissue were evaluated. Results:Following STZ dosing eNOS level dropped in the diabetic control group relative to the control group. Statistically significant increase in eNOS levels were seen in the LESWT+DM group. Similarly, in the diabetic control group STZ treatment decreased VEGF levels, while in the LESWT+DM group VEGF nearly approached to baseline levels. However variations in VEGF levels were not statistically significant. Conclusion:Mechanism action of ESWT in the penile tissue seems to involve angiogenic factors.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionsupporting

confidence: 76%

Effect of low-energy shockwave therapy on angiogenic factors in the penile tissue of diabetic rats

Ortaç

Küçükergin

Salabaş

et al. 2017

Turkish Journal of Urology

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“…It was thus shown that Li-ESWT triggers the activation of various intracellular signaling pathways causing upregulation of numerous angiogenic factors (i.e., VEGF…) to promote neovascularization and mobilizes the recruitment of endogenous progenitor cells thereby favoring angiogenesis and tissue regeneration in cardiovascular medicine and wound healing (17)(18)(19)(20)(21). These pro-angiogenic signaling pathways and recruitment of endogenous progenitor cells have also been shown to be activated in the penile tissue following Li-ESWT treatment in diabetic ED models (15,16), despite the fact that it does not upregulate the NO/cGMP pathway (14). Moreover, the role of Li-ESWT in the regeneration of peripheral nerves has also been previously suggested after nerve compression or in a model of autologous nerve graft (22,23) and later investigated in the context of erectile function recovery in diabetic ED models (15,16).…”

mentioning

confidence: 99%

“…These pro-angiogenic signaling pathways and recruitment of endogenous progenitor cells have also been shown to be activated in the penile tissue following Li-ESWT treatment in diabetic ED models (15,16), despite the fact that it does not upregulate the NO/cGMP pathway (14). Moreover, the role of Li-ESWT in the regeneration of peripheral nerves has also been previously suggested after nerve compression or in a model of autologous nerve graft (22,23) and later investigated in the context of erectile function recovery in diabetic ED models (15,16).…”

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confidence: 99%

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Low-energy Shock Wave Therapy Ameliorates Erectile Dysfunction in a Pelvic Neurovascular Injuries Rat Model

Matheu

Sun

et al. 2016

The Journal of Sexual Medicine

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113

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Introduction Erectile dysfunction (ED) caused by pelvic injuries is a common complication of civil and battlefield trauma with multiple neurovascular factors involved, and no effective therapeutic approach is available. Aims To test the effect and mechanisms of low-energy shock wave (LESW) therapy in a rat ED model induced by pelvic neurovascular injuries. Methods Thirty-two male Sprague-Dawley rats injected with 5-ethynyl-2′-deoxyuridine (EdU) at newborn were divided into 4 groups: sham surgery (Sham), pelvic neurovascular injury by bilateral cavernous nerve injury and internal pudendal bundle injury (PVNI), PVNI treated with LESW at low energy (Low), and PVNI treated with LESW at high energy (High). After LESW treatment, rats underwent erectile function measurement and the tissues were harvested for histologic and molecular study. To examine the effect of LESW on Schwann cells, in vitro studies were conducted. Main Outcome Measurements The intracavernous pressure (ICP) measurement, histological examination, and Western blot (WB) were conducted. Cell cycle, Schwann cell activation-related markers were examined in in vitro experiments. Results LESW treatment improves erectile function in a rat model of pelvic neurovascular injury by leading to angiogenesis, tissue restoration, and nerve generation with more endogenous EdU+ progenitor cells recruited to the damaged area and activation of Schwann cells. LESW facilitates more complete re-innervation of penile tissue with regeneration of neuronal nitric oxide synthase (nNOS)-positive nerves from the MPG to the penis. In vitro experiments demonstrated that LESW has a direct effect on Schwann cell proliferation. Schwann cell activation-related markers including p-Erk1/2 and p75 were upregulated after LESW treatment. Conclusion LESW-induced endogenous progenitor cell recruitment and Schwann cell activation coincides with angiogenesis, tissue, and nerve generation in a rat model of pelvic neurovascular injuries.

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Administration of secretome from human placental stem cell‐conditioned media improves recovery of erectile function in the pelvic neurovascular injury model

Matz

Thakker

et al. 2020

J Tissue Eng Regen Med

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Human placental stem cells (PSCs) enhance histological and functional recovery in a rodent erectile dysfunction (ED) model. We tested the hypothesis that bioactive factors secreted by PSC (i.e., the secretome) mediate functional recovery and that acellular-conditioned media (CM) from PSC culture (PSC-CM) could be used independently to facilitate functional and histological recovery. To identify factors relative to efficacy of PSC, a comparison of CM from PSC and three additional human stem cell populations was performed. CM from human PSC, amniotic fluid stem cells (AFSCs), adipose-derived stem cells (ADSC), and human umbilical vein endothelial cells (HUVECs) was assayed using a semi-quantitative human cytokine antibody array. Male rats, after surgically created ED by neurovascular injury, were randomly divided into four groups: vehicle control (phosphate-buffered saline [PBS]), PSC, PSC-CM, and serum-free media control (SFM) as control. Functional data on intracorporal and mean arterial pressure were obtained, and histological architecture was examined 6 weeks after single injection. PSCs were found to secrete at least 27 cytokines and growth factors at a significantly higher level than the other three cell types. Either single injection of PSC-CM or PSC significantly improved erectile functional recovery and histological architecture compared with SFM or PBS. Injection of the secretome isolated from human PSC improves erectile functional recovery and histological structure in a rat model of neurovascular injury-induced ED. Further characterization of the unique protein expression within the PSC-CM may help to identify the potential for a novel injectable cell-free therapeutic for applicable patients.

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Evaluation of the Effect of Different Doses of Low Energy Shock Wave Therapy on the Erectile Function of Streptozotocin (STZ)-Induced Diabetic Rats

Cited by 84 publications

References 32 publications

Effect of low-energy shockwave therapy on angiogenic factors in the penile tissue of diabetic rats

Effect of low-energy shockwave therapy on angiogenic factors in the penile tissue of diabetic rats

Low-energy Shock Wave Therapy Ameliorates Erectile Dysfunction in a Pelvic Neurovascular Injuries Rat Model

Administration of secretome from human placental stem cell‐conditioned media improves recovery of erectile function in the pelvic neurovascular injury model

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