Evaluation of the Effect of Two Chlorhexidine Preparations on Biofilm Bacteria In Vitro: A Three-Dimensional Quantitative Analysis

Shen, Ya; Qian, Wei; Chung, Christopher A.; Olsen, Ingar; Haapasalo, Markus

doi:10.1016/j.joen.2009.04.030

Cited by 114 publications

(174 citation statements)

References 32 publications

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“…In comparison to bacterial invasion models based on culturing techniques that cannot be replicated in the clinical setup [20], this new model provided penetration of bacteria deep into the dentinal tubules by centrifugation and has been implemented in our study to standardize the methodology. The power of centrifugation forces allows for a strong bacterial invasion into the dentin and allows for standardized measurements of live/dead bacteria when used with the CLSM to measure the efficacy of the disinfecting solutions.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Addition of an EPS Degrading Enzyme with and without Detergent to 2% Chlorhexidine on Disruption of Enterococcus faecalis Biofilm: A Confocal Laser Scanning Microscopic Study

Ganesh

Nagendrababu

John

et al. 2015

JCDR

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Section: Resultsmentioning

confidence: 99%

The Effect of Addition of an EPS Degrading Enzyme with and without Detergent to 2% Chlorhexidine on Disruption of Enterococcus faecalis Biofilm: A Confocal Laser Scanning Microscopic Study

Ganesh

Nagendrababu

John

et al. 2015

JCDR

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“…E. faecalis was used as a target microorganism because this pathogen is frequently retrieved from root canals with persistent endodontic infection and resists elimination with conventional antimicrobial agents [22]. However, the relevance to clinical conditions would be further improved by using multispecies biofilms as usually encountered in oral infections [23].…”

Section: Discussionmentioning

confidence: 99%

Repeated exposures to blue light-activated eosin Y enhance inactivation of E. faecalis biofilms, in vitro

Marinic

Manoil

Filieri

et al. 2015

Photodiagnosis and Photodynamic Therapy

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Background: In dentistry, antibacterial photodynamic therapy (a-PDT) has shown promising results for inactivating bacterial biofilms causing carious, endodontic and periodontal diseases. In the current study, we assessed the ability of eosin Y exposed to 3 irradiation protocols at inactivating Enterococcus faecalis biofilms, in vitro. Methods: E. faecalis biofilms formed on hydroxyapatite disks were incubated with eosin Y (10-80 M), then activated with blue light using different irradiation protocols. Biofilms exposed to continuous exposure were incubated for 40 min before being light-activated for 960 s. For the intermittent exposure, biofilms were exposed 4 times to the light/photosensitizer combination (960 s total) without renewing the photosensitizer. For repeated a-PDT, the same light dose was delivered in a series of 4 irradiation periods separated by dark periods; fresh photosensitizer was added between each light irradiation. After treatment, bacteria were immediately labeled with LIVE/DEAD BacLight Bacterial Viability kit and viability was assessed by flow cytometry (FCM). Results were statistically analyzed using one-way ANOVA and Tukey multiple comparison intervals (˛= 0.05). * Corresponding author. Results:The viability of E. faecalis biofilms exposed to 10 M eosin Y, was significantly reduced compared to controls (light only-eosin Y only). After a second exposure to blue light-activated eosin Y, viability significantly decreased from 58% to 12% whereas 6.5% of the bacterial biofilm remained live after a third exposure (p < 0.05). Only 3.5% of the bacterial population survived after the fourth exposure. Conclusions:The results of this study indicate that blue light-activated eosin Y can photoinactivate E. faecalis biofilms grown on hydroxyapatite disks. Also, repeated exposures to blue light-activated eosin Y were shown to significantly improve efficacy. Further studies seem warranted to optimize the antibacterial activity of blue light-activated eosin Y on major oral pathogens.

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“…It has been stated that low surface tension enables better penetration of the mixture into the dentinal tubules 11,12 . Moreover, several studies have indicated that the surfactant improves the performance of the solutions when compared to the same compounds without the surfactant [13][14][15] . Therefore, the detergent included in the QMix might have decreased the surface tension of the solution and led to the removal of more Ca(OH) 2 than the EDTA in the apical sections.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Qmix Solution in the Removal of Calcium Hydroxide From Artificially Created Grooves

KARATAŞ¹,

Arslan²,

UYGUN³

et al. 2018

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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Aim: The current study aimed to evaluate the effectiveness of QMix and EDTA solutions in removing Ca(OH) 2 from root canals. Materials and Methods:Forty-eight mandibular premolar teeth were instrumented by ProTaper Universal instruments. All of the teeth were then fixed in modified Eppendorf vials using a silicone impression material. After the removal of the specimens from the Eppendorf vials the roots were split into two halves longitudinally and standard longitudinal grooves were then created on the dentinal walls at a level of 3 mm below the cementoenamel junction and 3 mm above from the apex of the roots. The Ca(OH) 2 was placed into the grooves and the specimens were then remounted into the silicone impression material. Six groups were formed: Needle-EDTA, Needle-QMix, Ultrasonic-EDTA, Ultrasonic-QMix, Sonic-EDTA and Sonic-QMix. The root halves were separated and digital images of artificially created grooves were obtained with a stereomicroscope at a 25x magnification. The Ca(OH) 2 left on the artificially created grooves were scored using a 4-graded scoring system and the data were statistically analyzed.Results: Needle QMix group removed more Ca(OH) 2 than with the EDTA group at the apical part of the root canal (P < 0.083). Conclusions:When the irrigation was performed by a conventional needle, the QMix solution had better efficiency than EDTA in removing Ca(OH) 2 from the apical part of the root canal. In clinical practice, the QMix solution can be used effectively for the removal of Ca (OH) 2.

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Evaluation of the Effect of Two Chlorhexidine Preparations on Biofilm Bacteria In Vitro: A Three-Dimensional Quantitative Analysis

Cited by 114 publications

References 32 publications

The Effect of Addition of an EPS Degrading Enzyme with and without Detergent to 2% Chlorhexidine on Disruption of Enterococcus faecalis Biofilm: A Confocal Laser Scanning Microscopic Study

The Effect of Addition of an EPS Degrading Enzyme with and without Detergent to 2% Chlorhexidine on Disruption of Enterococcus faecalis Biofilm: A Confocal Laser Scanning Microscopic Study

Repeated exposures to blue light-activated eosin Y enhance inactivation of E. faecalis biofilms, in vitro

The Effect of Qmix Solution in the Removal of Calcium Hydroxide From Artificially Created Grooves

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