Evaluation of the Effect of an Amniotic Membrane Derived Bio-Nano Product (LifeCell) on Human Endometrial Cells Proliferation and Gene Expression: An in-vitro Study

Abstract

Background Successful assisted reproductive technology (ART) requires a receptive endometrium with appropriate thickness and the presence of specific cytokines, chemokines, and growth factors. Despite advancements in ART, the success rates remain suboptimal, particularly in individuals with thin endometrium resistant to treatment. Objective In this study, we evaluated the potential effects of LifeCell, a product of BioNano Technology, on the growth, development, and acceptance of endometrial cells. Method We cultured endometrial cells in a defined medium with different concentrations of LifeCell and examined cell growth, development, and the expression of genes involved in endometrial receptivity. Result Co-culture of primary human endometrial cells with 5% Life cell solution significantly stimulated the endometrial cell growth, development and receptivity genes expression. The expression levels of FGF2 and CSF in the 72 hours co-cultured were significantly increased compared with other groups (P <0.01). HOXA10 and LIF significantly increased in the 72 hours co-cultured compared with 24 hours co-cultured and control groups but had no significant level compared with 48 hours cocultured. HOXA10 significantly increased in the 48 hours cocultured compared with control group. IL-6 and Hb-EGF increased in the 48 hours co-cultured compared to other groups but had no significant level. VEGF increased in the treated groups compared to control but had no significant level. The expression of OPN, unlike the other genes, decreased in the treated group compared to the control, which was not significant. Conclusion These findings suggest that LifeCell may be a potential option for patients with treatment-resistant thin endometrium in cases of infertility.