Evaluation of the effect of routine histologic processing on the size of skin samples obtained from dogs

Reimer, S. Brent; Séguin, Bernard; DeCock, Hilde E. V.; Walsh, Peter; Kass, Philip H.

doi:10.2460/ajvr.2005.66.500

Cited by 44 publications

(67 citation statements)

References 21 publications

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“…This is similar to the pattern reported for dogs whereby the length and width decreased by 26.5% and the depth increased by 65.3%. 28 The decrease in length and width of skin samples is consistent with results of similar studies 11-13 of humans in which lateral margins decreased (range, 15% to 25% of the original dimension). However, an increase in depth of tissue has not been consistently found in human samples.…”

Section: Discussionsupporting

confidence: 90%

“…This finding was also noted in the canine study, 28 whereby the samples had a thicker depth but smaller width and length. A plausible explanation for the increase in depth of samples after tissue processing was the reexpansion and possible overexpansion that may have occurred when samples were placed in the water bath after sectioning with a microtome.…”

Section: Discussionsupporting

confidence: 62%

“…This study was closely modeled after a study 28 conducted on canine skin to allow for interspecies comparisons. Similar to that canine study, there was a decrease in length and width and an increase in depth of feline skin samples.…”

Section: Discussionmentioning

confidence: 99%

“…The neck, thorax, and tibia were chosen as locations to allow comparison with the canine study 28 and to represent 3 distinct locations. Skin samples obtained from the tibial location had a significantly greater amount of shrinkage in width and length, compared with shrinkage in width and length for skin samples obtained from the neck, at times 2, 3, and 4.…”

Section: Discussionmentioning

confidence: 99%

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Effect of histologic processing on dimensions of skin samples obtained from cat cadavers

Jeyakumar¹,

Smith²,

Schleis³

et al. 2015

ajvr

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OBJECTIVE To determine changes in dimensions of feline skin samples as a result of histologic processing and to identify factors that contributed to changes in dimensions of skin samples after sample collection. SAMPLE Cadavers of 12 clinically normal cats. PROCEDURES Skin samples were obtained bilaterally from 3 locations (neck, thorax, and tibia) of each cadaver; half of the thoracic samples included underlying muscle. Length, width, and depth were measured at 5 time points (before excision, after excision, after application of ink to mark tissue margins, after fixation in neutral-buffered 10% formalin for 36 hours, and after completion of histologic processing and staining with H&E stain). Measurements obtained after sample collection were compared with measurements obtained before excision. RESULTS At the final time point, tissue samples had decreased in length (mean decrease, 32.40%) and width (mean decrease, 34.21%) and increased in depth (mean increase, 54.95%). Tissue from the tibia had the most shrinkage in length and width and that from the neck had the least shrinkage. Inclusion of underlying muscle on thoracic skin samples did not affect the degree of change in dimensions. CONCLUSIONS AND CLINICAL RELEVANCE In this study, each step during processing from excision to formalin fixation and histologic processing induced changes in tissue dimensions, which were manifested principally as shrinkage in length and width and increase in depth. Most of the changes occured during histologic processing. Inclusion of muscle did not affect thoracic skin shrinkage. Shrinkage should be a consideration when interpreting surgical margins in clinical cases. 945).

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of histologic processing on dimensions of skin samples obtained from cat cadavers

Jeyakumar¹,

Smith²,

Schleis³

et al. 2015

ajvr

View full text Add to dashboard Cite

show abstract

“…However, the presence of those artifacts is supposed to be comparable in each specimen independently form the technique employed. Artifacts are caused by the traction with forceps on the edge of the tissue and they are not linked to thermal energy that causes contraction of collagen in the dermal tissue and subsequent possible irregular shrinking [12]. This distortion, together with changes associated with fixation and cutting artifacts, may have contributed to some of the irregular margins observed [13].…”

Section: Overallmentioning

confidence: 99%

810nm, 980nm, 1470nm and 1950nm diode laser comparison: a preliminary “ex vivo” study on oral soft tissues

et al. 2015

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The introduction of diode lasers in dentistry has several advantages, mainly consisting on the reduced size, reduced cost and possibility to beam delivering by optical fibers. At the moment the two diode wavelengths normally utilized in the dental field are 810 and 980 nm for soft tissues treatments. The aim of this study was to compare the efficacy of four different diode wavelengths: 810, 980, 1470 and 1950 nm diode laser for the ablation of soft tissues. Several samples of veal tongue were exposed to the four different wavelengths, at different fluences. The internal temperature of the soft tissues, in the area close to the beam, was monitored with thermocouple during the experiment. The excision quality of the exposed samples have been characterized by means of an optical microscope. Tissue damages and the cut regularity have been evaluated on the base of established criteria. The lowest thermal increase was recorded for 1950 nm laser. Best quality and speed of incision were obtained by the same wavelength. By evaluating epithelial, stromal and vascular damages for all the used wavelengths, the best result, in terms of "tissue respect", have been obtained for 1470 and 1950 nm exposures. From the obtained results 1470 and 1950 nm diode laser showed to be the best performer wavelengths among these used in this "ex vivo" study, probably due to their greatest affinity to water.

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Trimming Tumors for Diagnosis and Prognosis

Stromberg

Meuten

2016

Tumors in Domestic Animals

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Evaluation of the effect of routine histologic processing on the size of skin samples obtained from dogs

Cited by 44 publications

References 21 publications

Effect of histologic processing on dimensions of skin samples obtained from cat cadavers

Effect of histologic processing on dimensions of skin samples obtained from cat cadavers

810nm, 980nm, 1470nm and 1950nm diode laser comparison: a preliminary “ex vivo” study on oral soft tissues

Trimming Tumors for Diagnosis and Prognosis

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