Evaluation of the Effect of Enicostemma axillare Extract on Migration of MCF-7 Cell Line

Ravi, Kamalam; Giribabu, K.; Rajagopalan, Vijayaraghavan; Silambanan, Santhi

doi:10.7860/jcdr/2019/42371.13241

Cited by 2 publications

(1 citation statement)

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“…[11] The previous study shows the anti-migratory effect of Enicostemma axillare on Michigan Cancer Foundation-7 (MCF-7) cell line. [12] E. axillare (synonym -Enicostemma littorale) is a perennial herb. It has been used traditionally for many diseases.…”

Section: Original Articlementioning

confidence: 99%

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Giribabu¹

2020

AJP

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Introduction: With the increasing level of resistance to chemotherapy and radiotherapy and the associated toxicity of these therapies, the uses of plant-based compounds are gaining importance. This study was done to prove the apoptotic potential of Enicostemma axillare in breast cancer cells. Materials and Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was performed involving the treatment of Michigan Cancer Foundation-7 cells for 24 h with ethanolic extract of E. axillare (EEEA) and doxorubicin to assess the cell viability, lactate dehydrogenase (LDH) was done to assess cytotoxicity, ethidium bromide (EtBr) and acridine orange (AO) assay was done to assess nuclear morphology of apoptotic cells and the expression of proteins associated with apoptosis-Bad, Bcl2, Bax, CytoC, Caspase 3, 8, and 9 were analyzed by western blotting. The data are expressed as mean±SEM. Results and Discussion: IC 50 value for EEEA is 12.5 µg/ml, whereas for doxorubicin is 1 µg/ml. A significant increased level of LDH release is seen in treated groups. EEEA and doxorubicin decrease cell viability. In AO/EtBr staining, the live cells of the EEEA treatment were similar to that of the doxorubicin. Cells stained green represent viable cells, whereas bright red staining represents late apoptotic cells. EEEA down-regulated the expression of Bcl2 (antiapoptotic protein) and up-regulated the expression of Bad and Bax (proapoptotic proteins) in MCF-7 cell line compared to control cells. In this study, EEEA treatment significantly increased the protein expression of caspase-3, 8, and 9 compared to control. Conclusions: Our finding showed that EEEA induces extrinsic and intrinsic pathway mediated apoptosis. Thus, E. axillare raises new hope for its use in breast cancer therapy.

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Section: Original Articlementioning

confidence: 99%

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Giribabu¹

2020

AJP

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Molecular Actions of Enicostemma hyssopifolium Whole Plant Extract on HPV18-Infected Human Cervical Cancer (HeLa) Cells

Koralahalli,

Hussain,

Devarajan

et al. 2024

ACAMC

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Objective: Enicostemma hyssopifolium (E. hyssopifolium) contains several bioactive compounds with anti-cancer activities. This study was performed to investigate the molecular effects of E. hyssopifolium on HPV18-containing HeLa cells. Methods: The methanol extract of E. hyssopifolium whole plant was tested for cytotoxicity by MTT assay. A lower and higher dose (80 and 160 μg/mL) to IC50 were analyzed for colonization inhibition (Clonogenic assay), cell cycle arrest (FACS analysis), and induction of apoptosis (AO/EtBr staining fluorescent microscopy and FACS analysis) and DNA fragmentation (comet assay). The HPV 18 E6 gene expression in treated cells was analyzed using RT-PCR and qPCR. Results: A significant dose-dependent anti-proliferative activity (IC50 - 108.25±2 μg/mL) and inhibition of colony formation cell line were observed using both treatments. Treatment with 80 μg/mL of extract was found to result in a higher percent of cell cycle arrest at G0/G1 and G2M phases with more early apoptosis, while 160 μg/mL resulted in more cell cycle arrest at SUBG0 and S phases with late apoptosis for control. The comet assay also demonstrated a highly significant increase in DNA fragmentation after treatment with 160 μg/mL of extract (tail moments-19.536 ± 17.8), while 80 μg/mL of extract treatment showed non-significant tail moment (8.152 ± 13.0) compared to control (8.038 ± 12.0). The RT-PCR and qPCR results showed a significant reduction in the expression of the HPV18 E6 gene in HeLa cells treated with 160 μg/mL of extract, while 80 μg/mL did not show a significant reduction. Conclusion: The 160 μg/mL methanol extract of E. hyssopifolium demonstrated highly significant anti-cancer molecular effects in HeLa cells.

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Evaluation of the Effect of Enicostemma axillare Extract on Migration of MCF-7 Cell Line

Cited by 2 publications

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Molecular Actions of Enicostemma hyssopifolium Whole Plant Extract on HPV18-Infected Human Cervical Cancer (HeLa) Cells

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