Evaluation of the effectiveness of Rosmarinus officinalis (Lamiaceae) in the alleviation of carbon tetrachloride-induced acute hepatotoxicity in the rat

Sotelo-Felix, José I.; Martínez‐Fong, Daniel; Muriel, Pablo; Santillán, Ricardo López; Castillo, Daniel Del; Yahuaca, P

doi:10.1016/s0378-8741(02)00090-9

Cited by 119 publications

(87 citation statements)

References 37 publications

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“…Carnosol, one of the main active principles, was clearly identifi ed according to its retention time, confi rming the presence of this compound in the extract, as previously described (Sotelo et al, 2002). Quantitative HPLC analysis showed that carnosol content in the Rosmarinus offi cinalis extract was 3.02% (w/w).…”

Section: Hplc Analysissupporting

confidence: 68%

“…The extract was suspended in water at 200 mg/mL and intragastrically administered through an esophageal cannula to assure a dose of 200 mg/kg of body weight, which corresponds to 6.04 mg/kg/day of carnosol (Sotelo et al, 2002). Daily treatment with Rosmarinus offi cinalis was given using two schemes: the prevention model which involved simultaneous administration with CCl 4 for 12 weeks, and the reversion model, where the treatment started after cirrhosis was established, and lasted for 12 weeks.…”

Section: Methodsmentioning

confidence: 99%

“…Several methods have now been developed to obtain suitable extracts of Rosmarinus offi cinalis with high antioxidant activity (Cuvelier et al, 1996). The hepatoprotective effects have been shown in studies on experimental acute liver damage (Sotelo et al, 2002), and on liver experimental cirrhosis (Yahuaca et al, 2005). Carnosic acid also provides protection from the liver carcinogen afl atoxin A (Costa et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Oxidative stress modulation by Rosmarinus officinalis in CCl₄‐induced liver cirrhosis

Gutiérrez

Alvarado

Presno

et al. 2009

Phytotherapy Research

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Rosmarinus offi cinalis (Lamiaceae) possesses antioxidant activity and hepatoprotective effects, and so may provide a possible therapeutic alternative for chronic liver disease. The effect produced by a methanolic extract of Rosmarinus offi cinalis on CCl 4 -induced liver cirrhosis in rats was investigated using both prevention and reversion models. Over the course of the development of cirrhosis, the increased enzymatic activities of gammaglutamyl transpeptidase and alanine aminotransferase, and the rise in bilirubin levels caused by CCl 4 administration, were prevented by Rosmarinus offi cinalis co-administration. When the cirrhosis by oxidative stress was evaluated as an increase on liver lipoperoxidation, total lipid peroxides, nitric oxide in serum, and loss of erythrocyte plasma membrane stability, R. offi cinalis was shown to prevent such alterations. On cirrhotic animals treated with CCl 4 , histological studies showed massive necrosis, periportal infl ammation and fi brosis which were modifi ed by R. offi cinalis. These benefi ts on experimental cirrhosis suggest a potential therapeutic use for R. offi cinalis as an alternative for liver cirrhosis.

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Section: Hplc Analysissupporting

confidence: 68%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Oxidative stress modulation by Rosmarinus officinalis in CCl₄‐induced liver cirrhosis

Gutiérrez

Alvarado

Presno

et al. 2009

Phytotherapy Research

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“…CCl4'un düşük dozlarda uzun süre uygulandığında karaciğer hücrelerinde yağ dejenerasyonuna, yüksek dozlarda ise karaciğer hücrelerinin nekrozuna, hidropik dejenerasyona ve paranşimde kanamalara neden olduğu bildirilmektedir. (9,(12)(13)(14). CCl4 ile oluşturulan toksikasyonda hücre hasarı, lipit peroksidasyonundaki artışa bağlıdır ve CCl4'ün toksik etkisinin, serbest radikale dönüşümü ile başladığı belirlenmiştir.…”

Section: Introductionunclassified

“…CCl4 ile oluşturulan toksikasyonda hücre hasarı, lipit peroksidasyonundaki artışa bağlıdır ve CCl4'ün toksik etkisinin, serbest radikale dönüşümü ile başladığı belirlenmiştir. Son yıllarda yapılan çalışmalarda karaciğer hastalıklarında oksidatif stressin arttığını ve artan oksidatif stress ile karaciğer hasarı ve fibrozis arasında ilişki olduğunu bildirmişlerdir (13)(14)(15).…”

Section: Introductionunclassified

Ratlarda Karbon Tetraklorür ile İndüklenen Karaciğer Toksikasyonu Üzerine Resveratrol’ün Koruyucu Etkisinin Histopatolojik ve TUNEL Metodu ile Araştırılması

Yıldırım¹

2017

Atatürk Üniversitesi Veteriner Bilimleri Dergisi

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Öz: Bu çalışmada, deneysel olarak karbon tetraklorür ile hepatotoksisite oluşturulan ratların karaciğer dokuları üzerine güçlü antioksidan etkisine sahip olan Resveratrol'ün, histopatoloji ve TUNEL metodu ile koruyucu etkinliğinin araştırılması amaçlanmıştır. Bu amaçla, 32 adet erkek Wistar cinsi Albino rat kullanıldı. Denekler rastgele seçilerek, 1. grup kontrol, 2. Grup CCl4, 3. grup Resveratrol. 4. grup ise CCl4+ Resveretrol olmak üzere 4 gruba ayrıldı. Deneme sonunda hayvanlar anestezi altında sakrifiye edilerek histopatolojik analizler için karaciğer örnekleri %10 formalin solüsyonuna alındı. Yapılan histopatolojik incelemede kontrol ve Resveratrol grubunda ratların karaciğerleri normal histolojik yapıda olduğu tespit edildi. CCI4 grubunda ise karaciğerin periasiner bölgelerindeki hepatositlerde şiddeti hidropik dejenerasyon, steatozis ve kogulasyon nekrozu tesbit edildi. CCI4+ Resveretrol grubunda ratların karaciğerlerinde periasiner bölgede hepatositlerde hafif hidropik dejenerasyona rastlanırken nekrotik hepatositlere hiç rastlanmadı. Apoptozisi belirlemek amacıyla TUNEL metodu kullanıldı. TUNEL sonuçlarına göre kontrol ve Resveratrol grubunda karaciğerlerde birkaç hücrede pozitiflik tespit edildi. CCI4 grubunda karaciğerlerin periasiner bölgelerindeki hepatositlerde çok sayıda pozitif hücre belirlendi. CCl4+Resveretrol grubunda ratların karaciğerlerinde periasiner bölgede çok az sayıda pozitif hücreye rastlandı. İstatiski olarak gruplar arasındaki fark önemli (P<0.05) bulundu. Sonuç olarak, histopatolojik ve immunohistokimyasal bulgulara göre CCI4 neden olduğu oksidatif strese ve lipitperoksidasyonu sonucu oluşturulan karaciğer hasarına karşı Resveratrolün bu hasarı önemli düzeyde inhibe edici etkisinin olduğu belirlenmiştir.Anahtar Kelimeler: Apoptozis, Histopatoloji, Karbontetraklorür, Resveratrol, TUNEL metod. Investigation by Histopathological and TUNEL Method of the Protective Effect of Resveratrol on Carbon Tetrachloride Induced Liver Toxicity in RatsAbstract: In this study, it was aimed to investigate the protective effect of Resveratrol, which has a strong antioxidant effect on liver tissues, by histopathology and TUNEL method in experimental rats hepatotoxic with carbon tetrachloride. For this reason 32 male Wistar rats were used. Rats were randomly chosen and separated into 4 groups. First control, rats in second group CCl4, 3rd group Resveratrol 4th group CCl4+Resveretrol. At the end of the experiment, rats were euthanasied under anaesthesia and liver samples were taken in formalin solution 10%. According to histopathological examination, livers were in normal histopathological structure in the control and Resveratrol groups. In CCl4 group, severe hydropic degeneration in hepatocytes localised in the center regions of the liver, steatosis and coagulation necrosis were detected. In CCl4+Resveretrol group; while slight hydropic degeneration were detected in the central regions of livers, necrotic hepatocytes were not detected. TUNEL method were used in order to detect apoptosis. According to TU...

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Infusions of Portuguese medicinal plants: Dependence of final antioxidant capacity and phenol content on extraction features

et al. 2007

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Evaluation of the effectiveness of Rosmarinus officinalis (Lamiaceae) in the alleviation of carbon tetrachloride-induced acute hepatotoxicity in the rat

Cited by 119 publications

References 37 publications

Oxidative stress modulation by Rosmarinus officinalis in CCl₄‐induced liver cirrhosis

Oxidative stress modulation by Rosmarinus officinalis in CCl₄‐induced liver cirrhosis

Ratlarda Karbon Tetraklorür ile İndüklenen Karaciğer Toksikasyonu Üzerine Resveratrol’ün Koruyucu Etkisinin Histopatolojik ve TUNEL Metodu ile Araştırılması

Infusions of Portuguese medicinal plants: Dependence of final antioxidant capacity and phenol content on extraction features

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Evaluation of the effectiveness of Rosmarinus officinalis (Lamiaceae) in the alleviation of carbon tetrachloride-induced acute hepatotoxicity in the rat

Cited by 119 publications

References 37 publications

Oxidative stress modulation by Rosmarinus officinalis in CCl4‐induced liver cirrhosis

Oxidative stress modulation by Rosmarinus officinalis in CCl4‐induced liver cirrhosis

Ratlarda Karbon Tetraklorür ile İndüklenen Karaciğer Toksikasyonu Üzerine Resveratrol’ün Koruyucu Etkisinin Histopatolojik ve TUNEL Metodu ile Araştırılması

Infusions of Portuguese medicinal plants: Dependence of final antioxidant capacity and phenol content on extraction features

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Oxidative stress modulation by Rosmarinus officinalis in CCl₄‐induced liver cirrhosis

Oxidative stress modulation by Rosmarinus officinalis in CCl₄‐induced liver cirrhosis