Evaluation of the effectiveness of photodynamic therapy for the endodontic treatment of primary teeth: study protocol for a randomized controlled clinical trial

Abstract

BackgroundThe elimination of pathogenic microorganisms from the root canal system is one of the major steps required for successful endodontic treatment. The aim of the proposed study is to conduct a randomized, controlled, clinical trial for the clinical and radiographic evaluation of the effectiveness of photodynamic therapy during the endodontic treatment of primary teeth.MethodsThirty primary anterior teeth in children aged 3 to 6 years old will be randomly divided into 2 groups: a control group, which will receive conventional treatment, and an experimental group, which will be subjected to photodynamic therapy. Microbiological evaluations will be performed before and after endodontic treatment. Moreover, clinical and radiographic evaluations will be performed on the day of treatment as well as 1, 3 and 6 months after treatment. Comparisons will be made of the two study groups. The data will be tabulated and presented in a descriptive, analytical fashion. Depending on the distribution (normal or non-normal), either the t test, ANOVA or the Mann-Whitney test will be used for analysis of the variables. The Wilcoxon test will be used for comparisons before and after treatment. P values < 0.05 (95 % significance level) will be considered indicative of statistically significant differences.DiscussionAs successful endodontic treatment is directly related to intra-canal bacterial disinfection and consideringthe difficult task of endodontic treatment in primary teeth, often due to difficulties in controlling youngchildren, the internal anatomy of root canals and root resorption, the alternative of using PDT is a painless,easy-to-administer method that does not lead to microbial resistance and can assist in the achievement ofsuccessful endodontic treatment in primary teeth by eliminating the pain children can experience due to retreatmentas well as premature tooth loss.Trial registrationThe protocol for this study was registered with Clinical Trials number NCT02485210 on 30 july 2015.

Antimicrobial Photodynamic Therapy as a Co‐adjuvant in Endodontic Treatment of Deciduous Teeth: Case Series

“…This evaluation was performed counting the colony-forming units before and after the treatment, after the microbiological test. The microbiological analysis of this study was based on the protocol described by Mota et al (8).…”

“…The effectiveness of endodontic therapy is verified by the decontamination of the root canal, because the microbial agents promote the development and maintenance of the pathological process that damages the pulp and the periapical region . Mechanical instrumentation associated with chemical irrigation does not completely eliminate the microorganisms present in the root canal .…”

Evaluation of antimicrobial Effectiveness of EndoVac and Laser for treatment of infected permanent root Canals

“…Evidence suggests that lasers can penetrate to depths grater then 400μ inside the dentinal tubules and cause the killing of bacteria in these greater depths (21) . As well as the ability of the laser disinfection to inactivate any microorganisms that persist after irrigation routines have been completed as stated in previous work by Da Mota et al (22) . Moreover, Lasers apart from causing bactericidal effect also cause occlusion of the dentinal tubules and thereby closing all the avenues of bacterial reinfection (23) .…”

Activation of Alkaline Irrigation Fluids in Endodontics

“…There has also been interest in following endodontic irrigation with laser photothermal or laser photodynamic disinfection, on the basis that these may inactivate any microorganisms that persist after irrigation routines have been completed [ 19 ].…”