Evaluation of the Effectiveness of Mesenchymal Stem Cells of the Placenta and Their Conditioned Medium in Local Radiation Injuries

Брунчуков, В.; Астрелина, Т. А.; Usupzhanova, D.; Rastorgueva, A.; Kobzeva, I. V.; Nikitina, Victoria; Lishchuk, S. V.; Дубова, Е. А.; Павлов, К. А.; Brumberg, Valentin; Benderitter, Marc; Samoylov, A. S.

doi:10.3390/cells9122558

Cited by 7 publications

(5 citation statements)

References 7 publications

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“…Modeling of the relatively "soft" X-ray radiation of LRIs was carried out using an LNK-268 X-ray unit (RAP100-10) (Diagnostics-M, Moscow, Russia) with the following irradiation mode: dose, 110 Gy; a 0.1 mm thick aluminum filter; voltage, 30 kV; beam current, 6.1 mA; and dose rate, 21.1 Gy/min for 312 s (dose accuracy ± 5%, dose measurement uncertainty ± 6%) according to the previously proposed method [19,60,61], leading to a short latency period and chronic skin ulcers in the laboratory animals. After irradiation, the animals were seated in individual sterile boxes with an autonomous smart flow ventilation system (Tecniplast Group, Buguggiate, Italy) with unlimited access to water and food.…”

Section: Modeling Of Local Radiation Injuriesmentioning

confidence: 99%

Biodistribution of Mesenchymal Stromal Cells Labeled with [89Zr]Zr-Oxine in Local Radiation Injuries in Laboratory Animals

Astrelina,

Brunchukov,

Kodina

et al. 2023

Molecules

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Background: Tracking the migration pathways of living cells after their introduction into a patient’s body is a topical issue in the field of cell therapy. Questions related to studying the possibility of long-term intravital biodistribution of mesenchymal stromal cells in the body currently remain open. Methods: Forty-nine laboratory animals were used in the study. Modeling of local radiation injuries was carried out, and the dynamics of the distribution of mesenchymal stromal cells labeled with [89Zr]Zr-oxine in the rat body were studied. Results: the obtained results of the labelled cell distribution allow us to assume that this procedure could be useful for visualization of local radiation injury using positron emission tomography. However, further research is needed to confirm this assumption. Conclusions: intravenous injection leads to the initial accumulation of cells in the lungs and their subsequent redistribution to the liver, spleen, and kidneys. When locally injected into tissues, mesenchymal stromal cells are not distributed systemically in significant quantities.

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Section: Modeling Of Local Radiation Injuriesmentioning

confidence: 99%

Biodistribution of Mesenchymal Stromal Cells Labeled with [89Zr]Zr-Oxine in Local Radiation Injuries in Laboratory Animals

Astrelina,

Brunchukov,

Kodina

et al. 2023

Molecules

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“…57 In vivo experimental animal models can be used in the form of mice, 53 rats, 55 and pigs. 21 This method has been tested on animal models with various conditions, such as chronic wounds, 58 burns, 38 diabetic foot ulcers, 71 skin ulcers, 53 and radiation wounds, 72 which require special handlers. The results showed that wound treatment with secretome was able to accelerate wound healing by increasing the effect of cell migration and proliferation and triggering angiogenesis, re-epithelialization, neovascularization and deposition of collagen that play a role in the wound healing process.…”

Section: Wound Healingmentioning

confidence: 99%

Mesenchymal Stem Cell Secretome for Dermatology Application: A Review

Damayanti

Rusdiana

Wathoni

2021

CCID

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Secretome, also known as conditioned medium, is a secreted molecule from mesenchymal stem cells (MSCs) that has a variety of biological activities that can be used in various therapies, especially on the skin applications. A lack of conventional therapies makes secretome as a promising alternative therapy. The presence of growth factors, cytokines, and extracellular vesicles including microvesicles and exosomes in secretome has been widely reported, which serves in improving the proliferation and migration of cells to help in skin regeneration. Therefore, we were able to optimize the use of this secretome in a well-needed special review related to its work in addressing various skin problems. So, in this article, we discussed the benefits and biological activity of secretome on the skin application. This review was compiled based on the approval of several sites, such as Scopus, PubMed, Science Direct, and Google Scholar with the terms "MSC secretome for skin," "secretome for skin," "secretome dermatology," "secretome conditioned medium for skin," "secretome conditioned medium for skin wound," "secretome conditioned medium for aging," "secretome conditioned medium for hair growth," and "secretome conditioned medium for psoriasis." A total of 215 articles were collected for selection, of which 90 articles were used. Based on the results, it was concluded that secretome has a variety of useful activities to regenerate and repair tissue damage that have not been used on the skin, such as for wound healing, photoprotection, promotion of hair growth, psoriasis treatment, and other application as antimicrobial.

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“…Topical application of a mixture of different stem cells such umbilical cord mesenchymal stem cells and other regenerative medicine products in a patient with an irradiated perineal wound after anal squamous cell cancer and flap failure resulted in wound healing after 79 days [ 3 ]. Application of conditioned mesenchymal stroma cells in severe local radiation injuries accelerated wound healing [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Stem Cells Shows Antiinflammatory Effect in an Irradiated Random Pattern Flap Model

Müller-Seubert,

Fuchs,

Horch

et al. 2024

JPM

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Background: In reconstructive surgery, local flaps might develop tissue necrosis or partial flap loss especially after previous irradiation, which may be necessary in many tumor entities. The application of stem cells seems promising to improve flap perfusion and might be a possible solution to optimize flap survival. Methods: Twenty rats received harvesting of bilateral random pattern fasciocutaneous flaps. The right flaps received 20 Gy ionizing radiation 4 weeks prior to the surgery, while the left flaps served as the non-irradiated control. After flap harvest, four different stem cell mixtures (5 × 106 ASC, ASC-HUVEC, MSC, MSC-HUVEC) were applied under both right and left flaps using 1 mL fibrin glue as the delivery vehicle. Flap size and its necrotic area were examined clinically. Two weeks after the surgery, HE staining and immunohistochemical staining for CD68 and ERG, as well as PCR analysis (Interleukin 6, HIF-1α and VEGF), were performed. Results: Application of ASCs, ASCs-HUVECs and MSCs resulted in a lower number of CD68-stained cells compared to the no cell group. The expression of Hif1α was higher in the ASC group compared to those in the MSC and previously treated no cell groups. Treatment with MSCs and MSCs-HUVECs prevented shrinking of the flaps in this series. Conclusion: Application of ASCs, MSCs and ASCs-HUVECs was shown to have an antiinflammatory effect. Treatment with MSCs and MSCs-HUVECs can prevent early shrinking of the flaps.

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Evaluation of the Effectiveness of Mesenchymal Stem Cells of the Placenta and Their Conditioned Medium in Local Radiation Injuries

Cited by 7 publications

References 7 publications

Biodistribution of Mesenchymal Stromal Cells Labeled with [89Zr]Zr-Oxine in Local Radiation Injuries in Laboratory Animals

Biodistribution of Mesenchymal Stromal Cells Labeled with [89Zr]Zr-Oxine in Local Radiation Injuries in Laboratory Animals

Mesenchymal Stem Cell Secretome for Dermatology Application: A Review

Application of Stem Cells Shows Antiinflammatory Effect in an Irradiated Random Pattern Flap Model

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