Evaluation of the Effects of Mouthwash on the Morphology and Cell Viability of Osteoblast‐Like Cells

Abstract

This study evaluated the effects of multiple mouthwashes on the cellular viability or the morphology of preosteoblasts. Mouse calvarial osteoblast-like cells were cultured and treated with mouthwashes of (1) benzydamine hydrochloride; (2) cetylpyridinium chloride and benzalkonium chloride; (3) methyl salicylate, menthol, eucalyptol, and thymol; and (4) sodium fluoride, xylitol, and chitosan. The treatment times were 30 seconds, 90 seconds, and 270 seconds. Cell morphology was evaluated with a microscope, and the viability of the treated cells was analyzed quantitatively using a commercially available kit. The untreated control group exhibited well-stretched fibroblast-like morphology. Treatment with mouthwash resulted in morphological changes in all groups. Treatment with sodium fluoride resulted in more noticeable changes. Treatment with mouthwash for 30 seconds produced a significant decrease in cell viability. An increase in time to 90 and 270 seconds did not produce additional noticeable changes. To conclude, commercially available mouthwashes created changes in cell morphology and decreased the cell viability of osteoblast-like cells irrespective of ingredients and treatment time.

Facile Synthesis and Application of Ag-NPs for Controlling Antibiotic-ResistantPseudomonas spp. and Bacillus spp. in a Poultry Farm Environment

“…Additionally, the MBC of each dilution was measured following the Agar dilution method (Figures 2(c) and 2(d)), using 100 μl of dilutions of MIC without visible turbidity and incubated at 37 °C for 24 h. Finally, the lowest concentration at which the bacteria did not grow was considered as MBC. third passages were utilized for the determination of Ag-NPs treated cell viability following trypan blue exclusion assay [87,88].…”

Comparative Cytotoxicity of Menthol and Eucalyptol: An In Vitro Study on Human Gingival Fibroblasts

“…To our knowledge, there is no previous evidence on the comparative cytotoxicity of eucalyptol and menthol. Nonetheless, a recent study assessed the cytotoxicity of a mouthwash containing both substances together with methyl salicylate and thymol on osteoblast-like cells and found alterations in their cell morphology and reduced cell viability, irrespective of the exposure time [ 47 ]. Again, this stresses the importance of assessing the safety and potential effects of common components in daily products that contact oral tissues before their use.…”