Evaluation of the Effects of Hypo-Magnetic Fields on Mouse Macrophage RAW264 Cells

Abstract

Hypo-magnetic fields (HMFs) are static magnetic fields that are significantly weaker than the geomagnetic field and are found in interplanetary space, on Mars, and the Moon. Understanding the mechanism of the biological effects of HMFs is an important topic in magnetobiology. Human physiology studies have shown that HMFs cause various adverse health effects such as increased blood pressure and blood flow rate. Previous studies on the effects of HMFs on cells revealed a variety of effects, such as a change in growth rate, a decrease in mitochondrial membrane potential, and an increase in reactive oxygen species. A decrease in mitochondrial membrane potential (DFm) may seriously impair cellular activity by interfering with the production of ATP. Thus, a deeper investigation of the effects of HMFs on mitochondrial function could provide clues to understanding the adverse effects of HMFs. In the present study, we used mouse macrophage RAW264 cells to investigate the effects of an HMF on mitochondrial membrane potential, mitochondrial superoxide anion (O2-) production, and cell proliferation.

The effect of hypomagnetic field on survival and mitochondrial functionality of active Paramacrobiotus experimentalis females and males of different age

“…The mechanism by which the level of Δψ is modulated by SMF and HMF remains unclear. Nevertheless, it is known that HMF treatment decreases Δψ and consequently the viability of animal cells, (e.g., Fu et al, 2016;Srimai et al, 2020) which results in a range of adverse effects and severe dysfunctions in animals, including humans (for review, see, e.g., Erdman et al, 2021a). Considering that the magnetic field acts on elements with a magnetic moment, the decrease in Δψ is proposed to be caused by HMF interaction with electrons moving along the Frontiers in Physiology frontiersin.org mitochondrial respiratory chain, ions moving along ion channels, or proton translocation by the respiratory chain (Binhi and Prato, 2017;Ogneva et al, 2020).…”