Evaluation of the effects of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats undergoing segmental resection and anastomosis of the left colon

Morais, Pedro Henrique Alves de; Farias, Igor Eduardo Caetano de; Duraes, Leonardo; Carneiro, Fabiana Pirani; Oliveira, Paulo Gonçalves de; Sousa, João Batista de

doi:10.1590/s0102-86502012000100011

Cited by 2 publications

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“…The present results corroborate those of previous studies, i.e. that CO 2 pneumoperitoneum does not alter healing, thus reinforcing the safety of pneumoperitoneum for laparoscopy when used at adequate pressures 20 , 32 , 33 .…”

Section: Discussionsupporting

confidence: 92%

“…An environment pressurized with CO 2 may alter angiogenesis and inflammatory response processes and, thus, interfere with the inflammatory and proliferative phases of healing 20 , 33 . The results of this study are consistent with those of previous studies by our group that have analyzed healing by various parameters: histopathological analysis with hematoxylin-eosin staining, tensile strength, rupture pressure, and hydroxyproline scar concentration 20 , 32 . In all these studies, pneumoperitoneum has proven to be safe and not to interfere with wound healing.…”

Section: Discussionsupporting

confidence: 92%

“…Healing can also be evaluated by other methods, such as hydroxyproline dosage, hematoxylin-eosin staining histopathological analysis, and tensile strength measurement 29,30 . Since collagen is critical to tissue strength and integrity and is essential in all stages of healing; its measurement in wound deposition was selected for this study [17][18][19]31,32 .…”

Section: ■ Discussionmentioning

confidence: 99%

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Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?

et al. 2020

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Purpose To determine by histomorphometric analysis whether CO 2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (PRE: 30 min preoperatively; POST: 30 min postoperatively; PP: 30 min pre- and postoperatively; C: control group). CO 2 pneumoperitoneum was insufflated to 5 mmHg of pressure. A laparotomy was performed; 1 cm of the left colon was then resected, and an end-to-end anastomosis was performed to simulate surgical trauma, after which the abdominal wall was closed. On postoperative days 7 or 14, a sample of the abdominal wall was collected, stained with picrosirius red and observed under polarized light in an optical microscope. The amount of collagen was estimated by computerized histomorphometric analysis. Results There were no significant differences in collagen deposition between the control and experimental groups on postoperative days 7 (p=0.720) or 14 (p=0.933). The amount of collagen increased as expected in all groups between postoperative days 7 and 14 (p=0.0003). Conclusion At 5 mmHg, CO 2 pneumoperitoneum does not interfere with collagen deposition in abdominal wall surgical wounds in rats.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Section: ■ Discussionmentioning

confidence: 99%

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Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?

et al. 2020

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“…The anastomotic breaking strength (ABS) was evaluated with a vertical test apparatus called Versa Test (Mecmesin Versa Test, United Kingdom), coupled to a portable digital dynamometer in which the colonic anastomosis samples were attached and pulled with a speed of 25mm/min [12]. …”

Section: Methodsmentioning

confidence: 99%

Alcohol acute intoxication before sepsis impairs the wound healing of intestinal anastomosis: rat model of the abdominal trauma patient

et al. 2012

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IntroductionMost trauma patients are drunk at the time of injury. Up to 2% of traumatized patients develop sepsis, which considerably increases their mortality. Inadequate wound healing of the colonic repair can lead to postoperative complications such as leakage and sepsis.ObjectiveTo assess the effects of acute alcohol intoxication on colonic anastomosis wound healing in septic rats.MethodsThirty six Wistar rats were allocated into two groups: S (induction of sepsis) and AS (alcohol intake before sepsis induction). A colonic anastomosis was performed in all groups. After 1, 3 or 7 days the animals were killed. Weight variations, mortality rate, histopathology and tensile breaking strength of the colonic anastomosis were evaluated.ResultsThere was an overall mortality of 4 animals (11.1%), three in the group AS (16.6%) and one in the S group (5.5%). Weight loss occurred in all groups. The colon anastomosis of the AS group didn’t gain strength from the first to the seventh postoperative day. On the histopathological analysis there were no differences in the deposition of collagen or fibroblasts between the groups AS and S.ConclusionAlcohol intake increased the mortality rate three times in septic animals. Acute alcohol intoxication delays the acquisition of tensile strength of colonic anastomosis in septic rats. Therefore, acute alcohol intoxication before sepsis leads to worse prognosis in animal models of the abdominal trauma patients.

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Evaluation of the effects of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats undergoing segmental resection and anastomosis of the left colon

Cited by 2 publications

References 14 publications

Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?

Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?

Alcohol acute intoxication before sepsis impairs the wound healing of intestinal anastomosis: rat model of the abdominal trauma patient

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