Evaluation of the Effects of Strain-Specific Antigen Variation on the Accuracy of Serologic Diagnosis of
            <i>Helicobacter pylori</i>
            Infection

Marchildon, P; Sugiyama, Toru; Fukuda, Yoshiaki; Peacock, J; Asaka, Masahiro; Shimoyama, Takatoshi; Dy, Graham

doi:10.1128/jcm.41.7.3462.2003

Cited by 29 publications

(39 citation statements)

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“…This may be especially true in Asia (223,412). However, others insist on the importance of common antigens present between the strains (9) and recommend pooling antigens from strains representing the different groups (225,340).…”

Section: Detection Of H Pylori Antibodiesmentioning

confidence: 99%

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

2007

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SUMMARY The discovery of Helicobacter pylori in 1982 was the starting point of a revolution concerning the concepts and management of gastroduodenal diseases. It is now well accepted that the most common stomach disease, peptic ulcer disease, is an infectious disease, and all consensus conferences agree that the causative agent, H. pylori, must be treated with antibiotics. Furthermore, the concept emerged that this bacterium could be the trigger of various malignant diseases of the stomach, and it is now a model for chronic bacterial infections causing cancer. Most of the many different techniques involved in diagnosis of H. pylori infection are performed in clinical microbiology laboratories. The aim of this article is to review the current status of these methods and their application, highlighting the important progress which has been made in the past decade. Both invasive and noninvasive techniques will be reviewed.

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Section: Detection Of H Pylori Antibodiesmentioning

confidence: 99%

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

2007

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“…The high-molecular-weight cell-associated proteins used for JHM-CAP EIA were mixtures of proteins purified from four Japanese strains isolated in Hokkaido University, whereas those for HM-CAP EIA were proteins purified from a U.S. strain (197SR-US) isolated at the Baylor College of Medicine (15) according to a published procedure (6). In brief, crude extracts solubilized with 1% n-octyl alcohol-containing phosphate-buffered saline were dialyzed against buffered saline and centrifuged to obtain soluble proteins.…”

Section: Methodsmentioning

confidence: 99%

“…The diagnostic performances of JHM-CAP EIA and HM-CAP EIA on adult populations were reported using UBT as the gold standard. For the samples of Japanese populations, the performance of JHM-CAP EIA is slightly better than that of HM-CAP EIA (15,18), although there was no difference for the samples of a U.S. population (15). Antibodies in the sera of H. pylori-infected children could be analyzed by immunoblotting.…”

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confidence: 99%

“…In contrast, a commercially available urine-based EIA test, URINELISA, is highly reliable with 92.8% sensitivity and 95.8% specificity in reference to the 13 C-labeled urea breath test (UBT) and HpSA test on the same age group of a similar population (21). The difference in the sensitivity might be due to antigen preparation or strain variation; i.e., URINELISA antigen was made of sonic extracts of a Japanese isolate (21), whereas HM-CAP EIA was made of high-molecular-weight cell-associated proteins purified from a U.S. isolate (15).…”

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confidence: 99%

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A Strain-Specific Antigen in JapaneseHelicobacter pyloriRecognized in Sera of Japanese Children

Okuda

Sugiyama

Fukunaga

et al. 2005

Clin Vaccine Immunol

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An enzyme immuno assay (EIA) test based on Japanese strain-derived high-molecular-weight cell-associated proteins (JHM-CAP) was evaluated by comparing with a previously developed EIA test based on a U.S. strain-derived high-molecular-weight cell-associated proteins (HM-CAP). Serum samples of 131 Japanese asymptomatic children (mean age, 5.5 years; range, 0 to 21 years) were tested that include 43 positive and 88 negative children as judged by Helicobacter pylori stool antigen test (HpSA test). Both tests showed comparable and reliable specificities, but the sensitivity of JHM-CAP EIA, at 93.0%, was much higher than that of HM-CAP EIA, at 67.4%. More false-negative results of HM-CAP were obtained in children under 10 years of age. Immunoblot analysis revealed that the JHM-CAP but not the HM-CAP preparation had a 100-kDa antigen recognized by JHM-CAP positive sera. It was concluded that JHM-CAP EIA is highly accurate for the serodiagnosis of H. pylori infection in Japanese young children and that the high sensitivity of JHM-CAP EIA in contrast to HM-CAP EIA is due to the presence of a 100-kDa antigen in Japanese strains that may be recognized by the host immune system at an early stage of infection.

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“…However, its persistence in post-eradicated cases calls into question its use in differentiating acute or past infections or performing post-treatment evaluation. The geographical variation in H. pylori strains can further decrease the reliabilities of the test, although using antigens pooled with different strains would increase its reliability [47,48]. It may give a false positive test result in a low prevalence population due its low specificity, which has to be confirmed by other reliable tests (SAT or UBT).…”

Section: Igg Serology: Serum Antigen Markermentioning

confidence: 99%

Helicobacter pylori infection: A recent approach to diagnosis and management

Rana¹,

Wang²,

Li³

et al. 2017

J. Biomed

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Helicobacter pylori infection is the most common infection worldwide and is associated with simple dyspepsia, heartburn and peptic ulcer diseases, most commonly leading to upper gastrointestinal bleeding and, ultimately, to the severe complication of gastric malignancy. Ninety percent of duodenal ulcers and 70% of gastric ulcers are associated with helicobacter pylori infections. Noninvasive methods, such as the urea breath test (UBT-13C or 14C) and fecal antigen test (FAT), have high sensitivity and specificity for the diagnosis of H. pylori. However, 4-6 weeks of anti-secretary drugs (proton pump inhibitor) prior to testing can lead to false negative results. Invasive methods have benefits over noninvasive methods in the case of peptic ulcer diseases by taking samples for culture to determine their sensitivities and to stage disease progression to malignant transformation. Initially, standard triple regimen was the choice for treatment worldwide. However, high antibiotic resistances in various geographical regions have recently made standard quadruple therapy (bismuth-based) the preferred treatment. Recent studies have shown the promising benefits of 10-14 days of sequential and concomitant quadruple therapies as the first line option in high drug resistance areas, in cases of multidrug resistance or in prior treatment failure cases. Levofloxacin-based triple therapy, furazolidone-based regimens, and recent hybrid or rescue therapies are also beneficial towards the eradication of H. pylori infection as a second or third line therapy. Additionally, the use of probiotics and phytomedicines improve eradication rates when used with triple or quadruple therapies.

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Evaluation of the Effects of Strain-Specific Antigen Variation on the Accuracy of Serologic Diagnosis of Helicobacter pylori Infection

Cited by 29 publications

References 0 publications

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

A Strain-Specific Antigen in JapaneseHelicobacter pyloriRecognized in Sera of Japanese Children

Helicobacter pylori infection: A recent approach to diagnosis and management

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