Evaluation of The Effects of Allium Jesdianum Bacterial Endophytes on Some Bacterial and Fungal Pathogens

Abstract

Background and Aim:The endophytes are the fungi or bacteria that live within a plant in a symbiotic relationship. This study aimed to investigate the effect of isolated bacterial endophytes from Allium jesdianum on some bacterial pathogens such as Staphylococcus aureus, Escherichia coli as well as pathogenic fungi, including Trichophyton mentagrophytes and Candida albicans.Materials and Methods: Various parts of A. jesdianum including leaves, stems, onions and flowers were randomly collected, cleaned from any contamination and then cultured in a suitable culture medium. The endophyte colonies were purified using chloroform to analyze the anti-pathogenic properties of the structural agents of the bacterial endophyte. To evaluate the anti-pathogenicity of endophyte metabolites, the broth media of the endophyte bacteria was centrifuged and the supernatants were filtered through sterilization. Antibiogram test was performed by disk diffusion method (Kirby Bauer) and the sensitivity was compared. Data were statistically analyzed by t-test and ANOVA.Results: Eleven bacterial endophytes were obtained from various sections of A. jesdianum. The endophyte bacterium has an improved antimicrobial effect on T. mentagrophytes. Unlike E. coli, secretory metabolites of endophytic bacteria had an antimicrobial effect on S .aureus and C. albicans. Conclusion:It can be concluded that the bacterial endophytes of A. jesdianum can be considered to be potential and beneficial agents against human pathogens. A. jesdianum with anti-pathogenic activity could be a source to produce important anti-pathogenic compounds from an agricultural and pharmaceutical point of view.

Evaluation of Antifungal Effect of Allium jesdianum Bacterial Endophytes on Rhizopus stolonifer, Penicillium, Cladosporium, and Trichophyton mentagrophytes

“…Based on the isolates identified from A. jesdianum, the endophytes of this plant belong to six different species: S. warneri (AJB1), S. succinus (AJB2), P. thivervalensis (AJB3), B. subtilis (AJB4), A. lwoffii (AJB5), and P. brenneri (AJB6). Endophytic bacteria are found in many different plants, including numerous medicinal plants that host these bacteria (27,28). Several studies have aimed to identify and isolate endophytes from a wide range of medicinal plants.…”