Evaluation of the Effects of Photobiomodulation on Biomechanical Properties and Hounsfield Unit of Partial Osteotomy Healing in an Experimental Rat Model of Type I Diabetes and Osteoporosis

Mostafavinia, Ataroalsadat; Ahadi, Reza; Abdollahifar, Mohammad‐Amin; Ghorishi, Seyed Kamran; Jalalifirouzkouhi, Ali; Bayat, Mohammad

doi:10.1089/pho.2016.4191

Cited by 10 publications

(12 citation statements)

References 41 publications

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“…Consistently, an in vivo study could not show any positive effect of LLLT on HU and mechanical properties of fracture healing in an OVX rat model. Furthermore, a combined treatment of laser and alendronate significantly increased HU and the mechanical properties of the fracture healing . In the current study, a combination effect of LLLT and OT incubation provided a protective effect on the mitochondria and decreased the apoptosis rate compared to other treatment groups.…”

Section: Discussionsupporting

confidence: 53%

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Effect of low‐level laser therapy and oxytocin on osteoporotic bone marrow‐derived mesenchymal stem cells

Fallahnezhad

Piryaei

Darbandi

et al. 2017

J of Cellular Biochemistry

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Postmenopausal osteoporosis (OP) is a major concern for public health. Low-level laser therapy (LLLT) has a positive effect on the health of bone marrow mesenchymal stem cells (BMMSCs). The purpose of this study is to evaluate the influence of LLLT and oxytocin (OT) incubation-individually and in combination-on osteoporotic BMMSCs in ovariectomized rats. Twelve female rats were randomized into two groups to undergo either a sham surgery (sham group) or ovariectomy-induced osteoporosis (OVX group). MSCs harvested from the BM of healthy and OVX rats underwent culture expansion. There were five groups. In Groups one (sham-BMMSC) and two (OVX-BMMSC) the cells were held in osteogenic condition medium without any intervention. In the group three (OT), OT incubation with optimum dose was performed for 48 h (two times, 10 −12 molar). In Group four, lasertreated-OVX-BMMSCs were treated with optimum protocol of LLLT (one time, 1.2 J/cm 2 ). In Group five (laser + OT group), the OT incubation plus the laser irradiation was performed. The biostimulatory effect of LLLT is demonstrated by a significant increase in the viability of OVX-BMMSCs, cell cycle, and extracellular levels of Transforming growth factor beta (TGF-β), insulin-like growth factor-I (IGF-I), and Alkaline phosphatase (ALP) compared to control OVX-BMMSCs and/or the sham group. OT incubation and laser + OT incubation have a positive effect on OVXBMMSCs. However, LLLT is more effective statistically. We conclude that LLLT significantly improved cell viability, enhanced the osteogenic potential of the OVXBMMSCs, and increased the extracellular levels of the TGF-β, IGF-I, and ALP.

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Section: Discussionsupporting

confidence: 53%

“…However, some recent studies show that LLLT alone is not effective in reversing the detrimental effects of OP in vivo …”

Section: Introductionmentioning

confidence: 99%

Effect of low‐level laser therapy and oxytocin on osteoporotic bone marrow‐derived mesenchymal stem cells

Fallahnezhad

Piryaei

Darbandi

et al. 2017

J of Cellular Biochemistry

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“…Callus densities of bone defects were determined immediately after euthanizing. All tests were performed by an expert radiologist and bone density evaluations were recorded in Hounsfield Units (HU) [34]. The radiologist was unaware of the group divisions.…”

Section: Methodsmentioning

confidence: 99%

“…Latest progresses in imaging technologies for example computerized tomography (CT) scanning have made it possible to evaluate the healing process of the bones by measuring the Hounsfield unit (HU) of bone tissue [3435]. …”

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confidence: 99%

The effects of pentoxifylline adminstration on fracture healing in a postmenopausal osteoporotic rat model

et al. 2017

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Previous studies report positive effects of pentoxifylline (PTX) alone or in combination with other drugs on some pathologic bone diseases as well as an ability to accelerate osteogensis and fracture healing in both animal models and human patients. The aim of this present study was to evaluate the effects of PTX administration on Hounsfield unit and bone strength at catabolic response (bone resorbing) of a fracture in an experimental rat model of ovariectomy induced osteoporosis (OVX-D). Thirty adult female rats were divided into groups as follows: 1 (OVX, control, no treatment); 2 (OVX, sham: daily distilled water); 3 (OVX, daily alendronate: 3 mg/kg); 4 (OVX, twice daily 100 mg/kg PTX) and 5 (OVX, PTX+alenderonate). OVX was induced by bilateral ovariectomy in all rats. A complete standardized osteotomy of the right femur was made after 3.5 months. PTX and alendronate treatments were performed for eight weeks. Then, rats were euthanized and had its right femur subjected to computerized tomography scanning for measuring Hounsfield unit; eventually, the samples were sent for a three point bending test for evaluation of the bone strength. Administration of PTX with 200 mg/kg and alendronate alone and in combination showed no significant alteration in Hounsfield unit and biomechanical properties of repairing callus of the complete osteotomy compared with the control group. Results showed increased bending stiffness and stress high load mean values of repairing complete osteotomy in PTX-treated rats compared to the control OVX-D.

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“…Three studies were excluded due to the fact that they evaluated differentiation by alkaline activity measurement in their study (Coombe et al, 2001;Jawad et al, 2013;Korany et al, 2012). Seven other studies excluded because of utilization of diabetic (Mostafavinia et al, 2017a;Patrocínio-Silva et al, 2014) and osteoprotic (Bossini et al, 2011;Fredoni et al, 2017;Mohsenifar et al, 2016;Mostafavinia et al, 2017b;Scalize et al, 2015) rats as their animal models. Five studies assessed bone formation or osteointegration just by radiographic scans and histological analysis were also excluded (de Vasconcellos et al, 2016;Fekrazad et al, 2015;Gerbi et al, 2005;Gomes et al, 2015;Mandi c et al, 2015).…”

Section: Data Itemsmentioning

confidence: 99%

Molecular impacts of photobiomodulation on bone regeneration: A systematic review

Hosseinpour

Fekrazad

Arany

et al. 2019

Progress in Biophysics and Molecular Biology

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Photobiomodulation (PBM) encompasses a light application aimed to increase healing process, tissue regeneration, and reducing inflammation and pain. PBM is specifically aimed to modify the expression of cellular molecules; however, PBM impacts on cellular and molecular pathways especially in bone regenerative medicine have been investigated in scattered different studies. The purpose of the current study is to systematically review evidence on molecular impact of PBM on bone regeneration. A comprehensive electronic search in Medline, Scopus, EMBASE, EBSCO, Cochrane library, web of science, and google scholar was conducted from January 1975 to October 2018 limited to English language publications on administrations of photobiomodulation for bone regeneration which evaluated biological factors. In addition, hand search of selected journals was done to retrieve all articles. This systematic review was performed based on PRISMA guideline. Among these studies, five articles reported in vitro results, twelve articles were in vivo, and three of them were clinical trials. The data tabulated according to the type of markers (osteogenic markers, angiogenic markers, growth factors, and inflammation mediators). PBM's effects depend on many parameters which energy density is more important than the others. PBM can significantly enhance expression of osteocalcin, collagen, RUNX-2, vascular endothelial growth factor, bone morphogenic proteins, and COX-2. Although since the heterogeneity of the studies and their limitations, an evidence-based decision for definite therapeutic application of PBM is still unattainable, the findings of our review can help other researchers to ameliorate their study design and elect more efficient approach for their investigation.

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Evaluation of the Effects of Photobiomodulation on Biomechanical Properties and Hounsfield Unit of Partial Osteotomy Healing in an Experimental Rat Model of Type I Diabetes and Osteoporosis

Abstract: Combined treatment of PW laser and alendronate significantly enhanced bone repair in an experimental model rat of TIDM and OP.

Cited by 10 publications

References 41 publications

Effect of low‐level laser therapy and oxytocin on osteoporotic bone marrow‐derived mesenchymal stem cells

Effect of low‐level laser therapy and oxytocin on osteoporotic bone marrow‐derived mesenchymal stem cells

The effects of pentoxifylline adminstration on fracture healing in a postmenopausal osteoporotic rat model

Molecular impacts of photobiomodulation on bone regeneration: A systematic review

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