Evaluation of the efficacy of probiotic VSL#3 and synbiotic VSL#3 and yacon‐based product in reducing oxidative stress and intestinal permeability in mice induced to colorectal carcinogenesis

Abstract

The objective of the present study was to evaluate the effect of probiotic VSL#3 isolated or associated with a yacon-based product (synbiotic) on oxidative stress modulation and intestinal permeability in an experimental model of colorectal carcinogenesis. Forty-five C57BL/6J mice were divided into three groups: control (standard diet AIN-93 M); probiotic (standard diet AIN-93 M and multispecies probiotic VSL#3, 2.25 × 10 9 CFU), and synbiotic (standard diet AIN-93 M with yacon-based product, 6% fructooligosaccharides and inulin, and probiotic VSL#3, 2.25 × 10 9 CFU). The experimental diets were provided for 13 weeks. The probiotic and the yacon-based product showed antioxidant activity, with the percentage of DPPH radical scavenging equal to 69.7 ± 0.4% and 74.3 ± 0.1%, respectively. These findings contributed to reduce hepatic oxidative stress: the control group showed higher concentration of malondialdehyde (1.8fold, p = 0.007 and 1.5-fold, p = 0.035) and carbonylated protein (2-fold, p = 0.008 and 5.6-fold, p = 0.000) compared to the probiotic and synbiotic groups, respectively. Catalase enzyme activity increased 1.43-fold (p = 0.014) in synbiotic group.The crypt depth increased 1.2-fold and 1.4-fold with the use of probiotic and synbiotic, respectively, compared to the control diet (p = 0.000). These findings corroborate the reduction in intestinal permeability in the probiotic and synbiotic groups, as measured by the percentage of urinary lactulose excretion (CON: 0.93 ± 0.62% × PRO: 0.44 ± 0.05%, p = 0.048; and CON: 0.93 ± 0.62% × SYN: 0.41 ± 0.12%, p = 0.043). In conclusion, the probiotic and synbiotic showed antioxidant activity, which contributed to the reduction of oxidative stress markers. In addition, they protected the mucosa from damage caused by chemical carcinogen and reduced intestinal permeability. Practical Application:The relationship between intestinal health and the occurrence of various organic disorders has been demonstrated in many

Helicobacter pylori-Mediated Oxidative Stress and Gastric Diseases: A Review

“…Furthermore, the barrier function is systematically enhanced by probiotics, which improves immunity against HP infection ( Dargenio et al, 2021 ). It is well-known that a probiotic and prebiotic-rich diet is one of the main combination therapies for the modulation of intestinal health ( Cruz et al, 2021 ). It has been known to efficiently inhibit the process of oxidative stress and inflammation, following the increased level of claudin-3 and occludin, which contributes to the restoration of the gastrointestinal barrier function ( Cruz et al, 2020 ).…”

Synbiotics and Their Antioxidant Properties, Mechanisms, and Benefits on Human and Animal Health: A Narrative Review

“…Some probiotics such as Clostridium butyricum MIYAIRI 588, Lactiplantibacillus plantarum CAI6, and Lacticaseibacillus rhamnosus GG have been shown to successfully coordinate redox homeostasis in the host cell, resulting in increased overall antioxidant capacity [ 16 , 26 , 32 ]. It is also stated that probiotics can influence the redox status of the host by their capacity to: (i) chelate metal ions; (ii) activate the host’s antioxidant system in addition to having its antioxidant enzyme system; (iii) create metabolites with antioxidant activity, such as GSH and butyrate; (iv) mediate antioxidant signaling pathways; (v) regulate enzymes that produce reactive oxygen species; and (vi) regulate the intestinal microbiota [ 33 ]. Likewise, the antioxidant properties of prebiotics have been studied and demonstrated, for instance, on goat milk fermented by L. plantarum L60 [ 34 ].…”

“…Relevant study has been led on a synbiotic combining the multistrain probiotics VSL#3 (four strains of Lactobacillus , three strains of Bifidobacterium , and one strain of Streptococcus ) with the yacon-based product PBY, which contains high concentrated prebiotics FOS and inulin. The probiotics VSL#3 and the synbiotic VSL#3 with PBY had a high ability to trap DPPH radicals in vitro and in vivo, as evidenced by a considerable decrease in hepatic oxidative stress indicators and enhanced catalase activity [ 33 ].…”

“…One relevant example is the probiotics mix VSL3 # and its synbiotic association with yacon-based product rich in FOS and inulin, and their protection effects on mucosa from damage caused by chemical carcinogen and reduced intestinal permeability in mice induced to colorectal carcinogenesis. The CAT enzyme activity increases in synbiotic and probiotic groups compared to the control group, while the oxidative stress biomarkers such as MDA and carbonylated protein decreases [ 33 ]. One study evaluated the effect of the synbiotic composed of probiotic B. infantis and the prebiotic XOS against ulcerative colitis in colitis-induced mice compared to probiotics or prebiotics alone.…”

The Effects of Vernonia cinerea Less Extracts on Antioxidant Gene Expression in Colorectal Cancer Cells

“…Oxidative stress has been a focus point in cancer therapy. There are many reports on colorectal cancer therapy via oxidative stress reduction using natural compounds (Chok et al, 2021;Cruz et al, 2021;Koyuncu et al, 2021;Lau et al, 2021;Wang et al, 2021).…”