2019
DOI: 10.1177/0004563219828405
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Evaluation of the efficacy of various reagents in improving microRNA extraction

Abstract: Background MicroRNA has received considerable attention in the clinical context, and attempts are being made to use microRNA in clinical diagnosis. However, adequate quantities of microRNA required for analysis are challenging to isolate. We tested the effect of various reagents in improving microRNA extraction and compared their efficacy to that of a commercially available extraction kit (HighPure miRNA isolation kit, Roche). Methods We used the synthetic oligonucleotide miR-21 and formalin-fixed, paraffin-em… Show more

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“…Recently, the modulation of microRNA (miRNA) has raised increasing attention in sepsis. 5 , 6 miRNAs are non‐coding endogenous RNAs that are implicated in the diagnosis of sepsis via the control of various genes at the post‐transcriptional level through translational repression or mRNA degradation. 7 Some articles have mentioned that miRNAs could be considered to be a novel biomarker in sepsis.…”
Section: Introductionmentioning
confidence: 99%
“…Recently, the modulation of microRNA (miRNA) has raised increasing attention in sepsis. 5 , 6 miRNAs are non‐coding endogenous RNAs that are implicated in the diagnosis of sepsis via the control of various genes at the post‐transcriptional level through translational repression or mRNA degradation. 7 Some articles have mentioned that miRNAs could be considered to be a novel biomarker in sepsis.…”
Section: Introductionmentioning
confidence: 99%