Evaluation of the efficacy of humic acids to counteract the toxic effects of aflatoxin B1 in turkey poults

Maguey-González, Jesús Adonai; Nava-Ramírez, María de Jesús; Gómez-Rosales, Sergio; Ángeles, María de Lourdes; Solís-Cruz, Bruno; Hernández-Patlán, Daniel; Merino-Guzmán, Rubén; Hernandez-Velasco, Xochitl; Hernández-Ramírez, Juan Omar; Loeza, Ileana; Senas-Cuesta, Roberto; Latorre, Juan D.; Vázquez-Durán, Alma; Du, Xiangwei; Méndez-Albores, Abraham; Hargis, Billy M.; Téllez-Isaías, Guillermo

doi:10.3389/fvets.2023.1276754

Cited by 2 publications

(8 citation statements)

References 43 publications

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“…The observed benefits of HA on intestinal microbiota, gut integrity, ileal morphometry, and cellular immunity significantly strengthen the argument for their use as a practical and effective intervention against AFB 1 toxicity. These positive effects align with previous publications [ 12 , 13 ]. The improved villus height and width, indicative of enhanced nutrient absorption, corroborate the findings from Taklimi et al and López-García et al [ 14 , 15 ].…”

Section: Discussionsupporting

confidence: 92%

“…Recent in vitro and in vivo studies have established the efficacy of humic acids (HA) in mitigating the detrimental effects of AFB 1 in turkey poults. These benefits include an improved performance and a significant reduction in aflatoxicosis severity [ 12 , 13 ]. Given the promising findings regarding the benefits of HA in mitigating AFB 1 toxicity in poultry, the present study aimed to assess the impact of dietary HA supplementation on various aspects of turkey poult health (intestinal microbiota, gut integrity, ileum morphometry, and cellular immunity) when fed an AFB 1 -contaminated diet.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the Impact of Humic Acids on Intestinal Microbiota, Gut Integrity, Ileum Morphometry, and Cellular Immunity of Turkey Poults Fed an Aflatoxin B1-Contaminated Diet

Maguey-González,

Liu,

Zhang

et al. 2024

Toxins

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A recent study published data on the growth performance, relative weights of the organs of the gastrointestinal tract, liver histology, serum biochemistry, and hematological parameters for turkey poults fed an experimental diet contaminated with aflatoxin B1 (AFB1) and humic acids (HA) extracted from vermicompost. The negative effects of AFB1 (250 ng AFB1/g of feed) were significantly reduced by HA supplementation (0.25% w/w), suggesting that HA might be utilized to ameliorate the negative impact of AFB1 from contaminated diets. The present study shows the results of the remaining variables, as an extension of a previously published work which aimed to evaluate the impact of HA on the intestinal microbiota, gut integrity, ileum morphometry, and cellular immunity of turkey poults fed an AFB1-contaminated diet. For this objective, five equal groups of 1-day-old female Nicholas-700 turkey poults were randomly assigned to the following treatments: negative control (basal diet), positive control (basal diet + 250 ng AFB1/g), HA (basal diet + 0.25% HA), HA + AFB1 (basal diet + 0.25% HA + 250 ng AFB1/g), and Zeolite (basal diet + 0.25% zeolite + 250 ng AFB1/g). In the experiment, seven replicates of ten poults each were used per treatment (n = 70). In general, HA supplementation with or without the presence of AFB1 showed a significant increase (p < 0.05) in the number of beneficial butyric acid producers, ileum villi height, and ileum total area, and a significant reduction in serum levels of fluorescein isothiocyanate–dextran (FITC-d), a marker of intestinal integrity. In contrast, poults fed with AFB1 showed a significant increase in Proteobacteria and lower numbers of beneficial bacteria, clearly suggesting gut dysbacteriosis. Moreover, poults supplemented with AFB1 displayed the lowest morphometric parameters and the highest intestinal permeability. Furthermore, poults in the negative and positive control treatments had the lowest cutaneous basophil hypersensitivity response. These findings suggest that HA supplementation enhanced intestinal integrity (shape and permeability), cellular immune response, and healthier gut microbiota composition, even in the presence of dietary exposure to AFB1. These results complement those of the previously published study, suggesting that HA may be a viable dietary intervention to improve gut health and immunity in turkey poults during aflatoxicosis.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Assessment of the Impact of Humic Acids on Intestinal Microbiota, Gut Integrity, Ileum Morphometry, and Cellular Immunity of Turkey Poults Fed an Aflatoxin B1-Contaminated Diet

Maguey-González,

Liu,

Zhang

et al. 2024

Toxins

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“…This stock was subsequently used to contaminate the rest of the commercial feed, using 10 g of the stock per kg of feed. The composition of the diet was similar to previously reported data for turkeys (Maguey-González et al 2023 ). Batches of 30 kg were artificially contaminated to give a final content of 250 ng AFB 1 /g feed.…”

Section: Methodssupporting

confidence: 86%

“…Regarding the bursa/spleen ratio, there were no statistically significant differences between treatments. Maguey-González et al ( 2023 ) reported that the relative weight of the spleen and the bursa of Fabricius increased significantly in poults that were fed with a diet containing AFB 1 (250 ng AFB 1 /g feed). These results are in accordance with those obtained in the present study.…”

Section: Resultsmentioning

confidence: 99%

“…Serum analysis was performed with a spectrophotometer using commercially available kits (BioSystems, Barcelona, Spain) to determine total protein (TP), glucose (Glu), calcium (Ca), uric acid (UA), creatinine (CRE), blood urea nitrogen (BUN), and the enzymes alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), creatine kinase (CK), and glutamate dehydrogenase (GLDH). The determination of hematocrit (Hct), and cell count of leukocytes (WBCs), lymphocytes (Ls), basophils (Ba), monocytes (Mn), and heterophils (H) was performed using an automated hematology analyzer (Cell-Dyn 1700; Abbott Diagnostics, Abbott Park, IL, USA), following the recommendations of Maguey-González et al ( 2023 ). Finally, the bled poults were sacrificed by inhalation of 80% carbon dioxide, 5% oxygen, and 15% nitrogen (Coenen et al 2000 ).…”

Section: Methodsmentioning

confidence: 99%

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Efficacy of powdered alfalfa leaves to ameliorate the toxic effects of aflatoxin B1 in turkey poults

Nava-Ramírez,

Maguey-González,

Gómez-Rosales

et al. 2024

Mycotoxin Res

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This experiment was conducted to determine the effect of an adsorbent material based on powdered alfalfa leaves added in the aflatoxin B1 (AFB1)-contaminated diet of turkey poults on production parameters, blood cell count, serum biochemistry, liver enzymes, and liver histology. For this purpose, three hundred and fifty female Nicholas-700 poults were randomly assigned into five treatments: (1) Control, AFB1-free diet; (2) AF, diet contaminated with 250 ng AFB1/g; (3) Alfalfa, AFB1-free diet + 0.5% (w/w) adsorbent; (4) AF+alfalfa, diet contaminated with 250 ng AFB1/g + 0.5% (w/w) adsorbent, and (5) AF+ yeast cell wall (YCW), diet contaminated with 250 ng AFB1/g + 0.5% (w/w) of yeast cell wall (a commercial mycotoxin binder used as reference material). The in vivo efficacy of powdered alfalfa leaves was assessed during a 28-day period. In general, the addition of powdered alfalfa leaves in the AFB1-free diet gave the best performance results (body weight, body weight gain, and feed intake) and improved the values of total protein, glucose, calcium, creatinine, and blood urea nitrogen. Moreover, the addition of powdered alfalfa leaves in the AFB1-contaminated diet enhanced body weight and body weight gain and significantly reduced the feed intake, compared to the AF and AF+YCW groups. Additionally, significant alterations in serum parameters were observed in poults intoxicated with the AFB1, compared to the Control group. Furthermore, typical histopathological lesions were observed in the liver of the AF group, which were significantly ameliorated with the addition of powdered alfalfa leaves. Conclusively, these results pointed out that low inclusion of powdered alfalfa leaves in the contaminated feed counteracted the adverse effects of AFB1 in turkey poults.

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Evaluation of the efficacy of humic acids to counteract the toxic effects of aflatoxin B1 in turkey poults

Cited by 2 publications

References 43 publications

Assessment of the Impact of Humic Acids on Intestinal Microbiota, Gut Integrity, Ileum Morphometry, and Cellular Immunity of Turkey Poults Fed an Aflatoxin B1-Contaminated Diet

Assessment of the Impact of Humic Acids on Intestinal Microbiota, Gut Integrity, Ileum Morphometry, and Cellular Immunity of Turkey Poults Fed an Aflatoxin B1-Contaminated Diet

Efficacy of powdered alfalfa leaves to ameliorate the toxic effects of aflatoxin B1 in turkey poults

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