Evaluation of the efficacy of AgNOR as a proliferative marker in oral leukoplakia: A morphometric analysis

Garg, Kavita Nitish; Raj, Vineet; Chandra, Shaleen

doi:10.4103/0975-5950.117880

Cited by 5 publications

(5 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Oral epithelial cells destined for “pre‐malignant” change are those most likely to already possess an inherently high proliferative activity. Using in vitro labelling studies to characterize oral epithelial activity, we have demonstrated a significant increase in proliferation indices for both increasingly severe dysplasia and worsening grades of OSCC differentiation; overall, increasing numbers of epithelial cells committed to cell division are characteristic of dysplastic PMD lesions . Of particular interest is the additional observation that “apparently normal‐looking” mucosa from OSCC patients may also exhibit abnormal proliferation profiles similar to that seen in dysplastic or neoplastic tissue …”

Section: Proliferationmentioning

confidence: 94%

“…Using in vitro labelling studies to characterize oral epithelial activity, we have demonstrated a significant increase in proliferation indices for both increasingly severe dysplasia and worsening grades of OSCC differentiation [8][9][10] ; overall, increasing numbers of epithelial cells committed to cell division are characteristic of dysplastic PMD lesions. 11,12 Of particular interest is the additional observation that "apparently normal-looking" mucosa from OSCC patients may also exhibit abnormal proliferation profiles similar to that seen in dysplastic or neoplastic tissue. 8 "Pre-malignant fields" probably precede tumour formation, with OSCC arising in a field subclone following specific, additional genetic damage; the field expands through oral epithelium at the expense of normal surrounding mucosa due to its inherently high proliferation rate.…”

Section: Proliferationmentioning

confidence: 99%

See 1 more Smart Citation

Perspectives on oral squamous cell carcinoma prevention—proliferation, position, progression and prediction

Thomson

2018

J Oral Pathology Medicine

146

View full text Add to dashboard Cite

Squamous cell carcinoma arising from oral mucosal epithelium remains a lethal and deforming disease due to tumour invasion, oro-facial destruction, cervical lymph node metastasis and ultimate blood-borne dissemination. Worldwide, 300 000 new cases are seen each year, with a recent and significant rise in incidence affecting particularly the young. To rationalize perspectives on preventive strategies in oral cancer management, this study addresses a number of fundamental questions regarding carcinogenesis: proliferation-what epithelial cell changes precede tumour development? Position-why are certain oral sites so predisposed to cancer? Progression-why do some precursor lesions progress to invasive carcinoma and others do not? Prediction-how can we predict individual patient and/or lesion behaviour to prevent disease progression? By improving our understanding of oral carcinogenesis, can we thereby facilitate more effective primary, secondary and tertiary preventive strategies and ultimately reduce the global burden of oral squamous cell carcinoma (OSCC)?

show abstract

Section: Proliferationmentioning

confidence: 94%

Section: Proliferationmentioning

confidence: 99%

Perspectives on oral squamous cell carcinoma prevention—proliferation, position, progression and prediction

Thomson

2018

J Oral Pathology Medicine

146

View full text Add to dashboard Cite

show abstract

“…[ 43 ] The amount of AgNOR proteins can be exploited as a marker of cell proliferation and AgNOR quantification is a useful tool in the evaluation of the rate of cancer cell proliferation. [ 44 ] In the current study, the amount of AgNOR spots was substantially augmented in the nucleus of lung cancer mice induced by B(a)P, which specifies the hyperactivity of cell proliferative during cancer conditions. Our outcomes are in correlation with the previous report from another research group that during lung cancer, increased number of AgNOR may be related to high proliferative activity and aggressiveness of tumor cells.…”

Section: Discussionmentioning

confidence: 59%

Baicalein inhibits cell proliferation and enhances apoptosis in human A549 cells and benzo(a)pyrene‐induced pulmonary carcinogenesis in mice

Naveenkumar

Raghunandhakumar

Devaki

2022

J Biochem & Molecular Tox

View full text Add to dashboard Cite

Our current study is done to explore the possible mechanisms to elaborate on the growth inhibitory effect of baicalein (BE) in human lung carcinoma. Initially, BE (25 and 50 µM) treatment for 24 h, suppressed the viability and inhibited population growth in A549 cells. BE upholds the production of reactive oxygen species (ROS) with concomitant replenishment of glutathione, catalase, and glutathione peroxidase activity. The expression level of nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 markedly increased after BE treatment will intimidate A549 cells proliferation by the ROS-independent pathway via the antioxidant pathway. In vivo investigations were carried out on BE (12 mg/kg, oral) in benzo(a)pyrene (B(a)P; 50 mg/kg, oral) induced lung carcinogenesis in mice. BE induces caspase-dependent apoptosis by increasing the levels of cytosolic cytochrome c accompanied by upregulating the outflow of p53, Bax, and caspase-3 with a concomitant abatement in the outflow of Bcl-2 in both in vitro and in vivo. In the murine model, BE treatment hindered the countenance of proliferation-related proteins (argyrophilic nucleolar organizing regions and proliferating cell nuclear antigen). Additionally, appraisal of the cell nucleus by transmission electron microscopic assessment uncovered that BE treatment adequately counteracts B(a)P-induced lung cancer cell survival. During the transition of the G 0 /G 1 phase, BE is arrested in the cell cycle process. This might be the cause of a substantial increase in the appearance of p21 Cip1 with concomitant downregulating the expressions of CDK4, cyclin D, and cyclin E both in vitro and in vivo. Our results conclude that BE treatment induced apoptosis and repressed proliferation both in vitro and in vivo of human lung carcinoma.

show abstract

“…Several reports have suggested that variations in AgNOR counts may reflect the state of activation or degree of malignancy of the lesion involved [ 16 ]. The AgNOR count is the most appropriate marker to differentiate between dysplastic and nondysplastic leukoplakia [ 17 ]. AgNOR analysis may be useful as a quantitative marker of incipient cellular alterations and hence would be helpful in assessing suspicious lesions and thus can be regarded as a valuable adjunct [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study of Morphometric Analysis of Nucleolar Organizer Regions in Oral Leukoplakia and Oral Squamous Cell Carcinoma and Significance of AgNOR as a Diagnostic Tool

Singh,

Soni

et al. 2023

Cureus

View full text Add to dashboard Cite

Background Silver stainable nucleolar organizer regions (AgNORs) have proven to exhibit utmost importance due to their higher occurrence in the nucleus especially in malignant cells than in normal. Thus, they assist in the examination of nucleolar structures and variations in nucleolar activity. Aim Quantitative and qualitative analysis in relation to the number and area of AgNOR in tissue sections of the normal oral mucosa (NOM), oral leukoplakia (OL), and oral squamous cell carcinoma (OSCC) was the main aim of the study. Materials & method A total of 50 cases comprising 20 OL with dysplasia, 20 OSCC cases, and 10 samples of normal oral mucosa were taken. Silver nitrate (Sol A) & gelatin (Sol B) solutions were freshly prepared for staining the lesional slides. Results The mean value of nuclear profile area (A Nuc) was comparatively higher in oral leukoplakia i.e. 41.97 and in oral squamous cell carcinoma i.e. 62.36 in comparison to the control group where it was 36.19. The mean value of a single AgNOR profile area per nucleus (A NOR) was found to be comparatively lower in both study groups i.e. oral leukoplakia (2.76) and oral squamous cell carcinoma (1.61) in comparison to the control group (3.45) . The mean value of total AgNOR profile area per nucleus (TA NOR) and the number of profiles of AgNORs per nucleus (n NOR) were found higher in both study groups (oral leukoplakia and oral squamous cell carcinoma) as compared to normal oral mucosa of the control group. However, the findings of all four parameters of morphometric analysis were found to be significantly associated with disorder of oral mucosa i.e. cases of oral leukoplakia and oral squamous cell carcinoma (P value <0.01). Conclusion It can thus be suggested that the mean AgNOR count displayed a higher value in OSCC. Hence, the number of AgNORs in nuclei increases as epithelial cells undergo malignant transformation which is designated that mean AgNOR count may contribute to establishing the prognosis of a lesion.

show abstract

Evaluation of the efficacy of AgNOR as a proliferative marker in oral leukoplakia: A morphometric analysis

Cited by 5 publications

References 16 publications

Perspectives on oral squamous cell carcinoma prevention—proliferation, position, progression and prediction

Perspectives on oral squamous cell carcinoma prevention—proliferation, position, progression and prediction

Baicalein inhibits cell proliferation and enhances apoptosis in human A549 cells and benzo(a)pyrene‐induced pulmonary carcinogenesis in mice

A Comparative Study of Morphometric Analysis of Nucleolar Organizer Regions in Oral Leukoplakia and Oral Squamous Cell Carcinoma and Significance of AgNOR as a Diagnostic Tool

Contact Info

Product

Resources

About