Evaluation of the efficacy of Myco/F lytic system, MGIT960 system and Lowenstein-Jensen medium for recovery of Mycobacterium tuberculosis from sterile body fluids

Wang, Guirong; Yang, Xinting; Zhu, Junping; Dong, Wei; Huang, Mailing; Jiang, Guanglu; Zhao, Liping; Qin, Sibing; Chen, Xiaoyou; Huang, Hairong

doi:10.1038/srep37757

Cited by 7 publications

(15 citation statements)

References 16 publications

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“…851 specimens were sent for mycobacterial culture using MGIT 960 and novel culture method base on BACTEC Myco/F Lytic culture system at the meantime, positive rate of MGIT 960 (48. 5.64%) was also higher than novel culture method (12, 1.41%) with P value < 0.001, which was opposite with previous report (3). Another study showed similar positive rate for pleural fluid tuberculosis detected by using MGIT 960 and Myco/F lytic liquid culture system (9).…”

Section: Discussioncontrasting

confidence: 85%

“…The important fungal media in clinical practice are Sabouraud’s medium agar for sputum, bronchoalveolar lavage fluid (BALF) and urine. Furthermore, BACTEC Myco/F Lytic culture system could be used to grow fungi and mycobacteria from blood and sterile body fluid (2, 3). For clinical specimens inoculated on culture agar, only fast-growing fungi with high capacity of sporulation can be detected (4), while slow-growing fungi are usually overlooked (5).…”

Section: Introductionmentioning

confidence: 99%

“…This maybe the reasons why only a few species of molds and yeasts are isolated in some clinical microbiology laboratories (6). The Myco/F lytic system is an automated, continuous-monitoring system for fungal and mycobacterial culture, which has been used for detection of bacteria (2), fungi (7, 8) and mycobacteria in blood (2) and sterile body fluid specimens (3, 9). Therefore, considering the small size of the tissue often gained in clinical microbiology laboratory, Myco/F lytic system might be used for the culture of bacteria and fungi after reasonable sample preparation.…”

Section: Introductionmentioning

confidence: 99%

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Novel culture method to diagnose fungal infections with biopsy tissues

Zhao

Zhang

Fan

et al. 2020

Preprint

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Biopsy tissue is a difficult sample to gain for the identification of potential pathogen in the clinical laboratory. At present, there are no effective culture methods for small-sized biopsy tissue. In this study, we propose a novel tissue biopsy culture method based on Myco/F lytic liquid culture system, namely, tissue was grinded before injected into culture bottles. More types and numbers of fungi are cultivated using this culture system than sputum or BALF culture. A few of mycobacteria were also detected with ground tissues. The method may be a promising alternative to or supplement for the traditional plate culture in clinical practice.

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Section: Discussioncontrasting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel culture method to diagnose fungal infections with biopsy tissues

Zhao

Zhang

Fan

et al. 2020

Preprint

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“…It has been reported that liquid media-based culture has better performance than solid culture in terms of the isolation rate 20 . In other studies, MGIT 960 demonstrated better sensitivity than solid culture 21,22 . In fact, MGIT 960 was equivalent to the BACTEC 460 system, the gold standard for MTB (but discontinued by the manufacturer) 20–22 .…”

Section: Discussionmentioning

confidence: 77%

“…The results of Xpert testing were first compared with those of the gold standard (culture) 6 , i.e., those of MGIT 960 culture-positive cases. MGIT 960 had demonstrated better sensitivity than solid culture and was equivalent to the BACTEC 460 system, the previous gold standard for MTB, which has been discontinued by the manufacturer 20–22 . Given that culture is suboptimal in detecting EPTB, a composite reference standard (CRS) was also used as a standard for comparison, as recommended in the literature in the absence of an ideal gold standard 16,17,23 .…”

Section: Methodsmentioning

confidence: 99%

Multicentre evaluation of Xpert MTB/RIF assay in detecting urinary tract tuberculosis with urine samples

Chen¹,

et al. 2019

Sci Rep

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Genitourinary tuberculosis (GUTB) accounts for up to 40% of extrapulmonary tuberculosis cases. Rapid tests for GUTB are urgently needed because it is often associated with delayed health-care seeking, leading to serious consequences. This study evaluated the performance of the Xpert MTB/RIF assay in the rapid diagnosis of urinary tract tuberculosis (UTB) and rifampicin-resistant tuberculosis with urine specimens. In all, 302 patients were included from four hospitals in China. Suspected UTB patients were tested with Xpert, smear, and MGIT 960 culture. Drug susceptibility testing (DST) was conducted for culture-positive cases. The performance of the assays was evaluated against MGIT 960 culture and a composite reference standard (CRS). Among all participants, 150 (49.7%) had CRS-positive UTB, of whom 36 (24.0%) were culture-confirmed. Against culture, Xpert and smear achieved a sensitivity of 94.4% (95% CI: 81.3–99.3%) and 22.2% (95% CI: 10.1–39.2%), respectively. Against CRS, the sensitivity of Xpert, smear and culture was 41.3% (95% CI: 33.4–49.7%), 7.3% (95% CI: 3.7–12.7%), and 24.0% (95% CI: 17.4–31.6%). Xpert had better performance than smear and culture in detecting UTB from urine samples and could be considered for the diagnosis of UTB. Moreover, Xpert showed better performance than MGIT 960-based DST using urine culture.

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Xpert MTB/RIF Ultra enhanced tuberculous pleurisy diagnosis for patients with unexplained exudative pleural effusion who underwent a pleural biopsy via thoracoscopy: A prospective cohort study

Gao

Wang

Yu³

et al. 2021

International Journal of Infectious Diseases

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Introduction: To evaluate the performance of Xpert MTB/RIF Ultra (Xpert-Ultra) in testing pleural tissue and fluid collected by medical thoracoscopy among patients with unexplained exudative pleural effusion. Methods: Patients with an undiagnosed exudative pleural effusion were prospectively and consecutively recruited. Pleural tissue and fluid were collected by medical thoracoscopy and subjected to culture, Xpert MTB/RIF (Xpert) and Xpert-Ultra assays. Histopathological examination was also performed with the tissue and used as the major reference.Results: Sixty-one patients were enrolled, including: 27 tuberculosis (TB) pleurisy, 15 malignancy and 19 other chronic infection cases. The sensitivity, specificity, positive predictive value, and negative predictive value of Xpert-Ultra for TB diagnosis were 85.19% (23/27), 97.06% (33/34), 95.83% (23/24), and 89.19% (33/37), respectively. Xpert-Ultra testing with the biopsy tissue alone had an equivalent diagnostic capacity to that of pathological examination for the diagnosis of confirmed TB cases. By combining the pathological examination with Xpert-Ultra for biopsy, the percentage of confirmed TB cases greatly increased (i.e. 92.59% (25/27)). The "trace" positive outcome of Xpert-Ultra was highly supportive of TB diagnosis for both biopsy tissue and pleural fluid examinations. Conclusion: With the specimens collected by medical thoracoscopy, the Xpert-Ultra assay presented high value in identifying TB among pleurisy patients who had difficulties in etiological diagnosis.

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Evaluation of the efficacy of Myco/F lytic system, MGIT960 system and Lowenstein-Jensen medium for recovery of Mycobacterium tuberculosis from sterile body fluids

Cited by 7 publications

References 16 publications

Novel culture method to diagnose fungal infections with biopsy tissues

Novel culture method to diagnose fungal infections with biopsy tissues

Multicentre evaluation of Xpert MTB/RIF assay in detecting urinary tract tuberculosis with urine samples

Xpert MTB/RIF Ultra enhanced tuberculous pleurisy diagnosis for patients with unexplained exudative pleural effusion who underwent a pleural biopsy via thoracoscopy: A prospective cohort study

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