Evaluation of the EIE-IgM-Leptospirose assay for the serodiagnosis of leptospirosis

McBride, Alan J. A.; Pereira, Fernanda Ávila da Costa; Silva, Emilson D. da; Matos, Rosan Barbosa de; Silva, Edimilison D. da; Ferreira, Antônio G. P.; Reis, Mitermayer Galvão dos; Ko, Albert I.

doi:10.1016/j.actatropica.2007.05.002

Cited by 18 publications

(14 citation statements)

References 33 publications

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“…Our evaluation was conducted from a single site and therefore may not necessarily apply to other urban settings of endemicity. However, the study's findings are consistent with those of evaluations of in-house assays or individual commercial kits which were performed with predominantly urban-based subjects from other sites (3,22,25,28,32).…”

supporting

confidence: 85%

Evaluation of Four Whole-Cell Leptospira -Based Serological Tests for Diagnosis of Urban Leptospirosis

McBride

Santos

Queiroz

et al. 2007

Clin Vaccine Immunol

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Four serologic assays for leptospirosis had sensitivities of 72 to 88% and specificities of 88 to 100% in the setting of highly endemic urban transmission, indicating that assays using enzyme-linked immunosorbency and rapid formats may be used as alternatives to the microscopic agglutination test for diagnosing urban leptospirosis. Testing a second sample will be required in cases with an initial negative result, since sensitivity was low (46 to 68%) during the first week of illness.

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supporting

confidence: 85%

Evaluation of Four Whole-Cell Leptospira -Based Serological Tests for Diagnosis of Urban Leptospirosis

McBride

Santos

Queiroz

et al. 2007

Clin Vaccine Immunol

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“…38 The anti-Leptospira immunoglobulins develop during the first 10 d following infection, the IgM peak is quickly followed by increasing IgG levels. 3,7 The antibodies persist in patient serum for up to five years 41,42 and even low antibody levels are protective. 3,40 Conversely, a humoral immune response with a high titer of agglutinating antibodies does not protect cattle, rather a cellular immune response is required for protection against leptospirosis.…”

Section: The Immune Responsementioning

confidence: 99%

Recombinant vaccines against Leptospirosis

et al. 2011

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Leptospirosis is an important neglected infectious disease that occurs in urban environments, as well as in rural regions worldwide. Rodents, the principal reservoir hosts of pathogenic Leptospira spp., and other infected animals shed the bacteria in their urine. During occupational or even recreational activities, humans that come into direct contact with infected animals or with a contaminated environment, particularly water, are at risk of infection. Prevention of urban leptospirosis is largely dependent on sanitation measures that are often difficult to implement, especially in developing countries. Vaccination with inactivated whole-cell preparations (bacterins) has limited efficacy due to the wide antigenic variation of the pathogen. Intensive efforts towards developing improved recombinant vaccines are ongoing. During the last decade, many reports on the evaluation of recombinant vaccines have been published. Partial success has been obtained with some surface-exposed protein antigens. The combination of protective antigens and new adjuvants or delivery systems may result in the much-needed effective vaccine.

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“…Blood samples were tested for the presence of anti- Leptospira IgM antibodies by an ELISA (Bio-Manguinhos, Rio de Janeiro, Brazil) (McBride et al, 2007). This assay is used by the Brazilian Ministry of Health for laboratory confirmation of leptospirosis and has a sensitivity of 100% in identifying antibodies during the convalescent phase of the illness among cases confirmed by the standard MAT.…”

Section: Methodsmentioning

confidence: 99%

Leptospirosis in a subsistence farming community in Brazil

Lacerda

Monteiro

Oliveira

et al. 2008

Transactions of the Royal Society of Tropical Medicine and Hygi

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Summary Leptospirosis has been reported in rural areas of Brazil. However, there is limited information about the exposure risk or the risk of Leptospira infection for rural-based populations. A cross-sectional study was carried out in order to determine the prevalence and risk factors for prior Leptospira infection in a rural subsistence farming region of the state of Rio Grande do Norte, an area in which outbreaks of leptospirosis have occurred. Among 290 individuals enrolled, 44 (15.2%) had anti-Leptospira IgM antibodies as determined by IgM ELISA. Infection tended to occur with activities related to the rice fields (P = 0.08). Our findings indicate that Leptospira infection occurs even in years of low rainfall, and may have an important impact among poor rural-based subsistence farmers in Brazil. Additional studies are needed to characterize the mode of transmission in this region.

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Evaluation of the EIE-IgM-Leptospirose assay for the serodiagnosis of leptospirosis

Cited by 18 publications

References 33 publications

Evaluation of Four Whole-Cell Leptospira -Based Serological Tests for Diagnosis of Urban Leptospirosis

Evaluation of Four Whole-Cell Leptospira -Based Serological Tests for Diagnosis of Urban Leptospirosis

Recombinant vaccines against Leptospirosis

Leptospirosis in a subsistence farming community in Brazil

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