Evaluation of the elastic Young’s modulus and cytotoxicity variations in fibroblasts exposed to carbon-based nanomaterials

Pastrana, Fernando; Cartagena‐Rivera, Alexander X.; Raman, Arvind; Ávila, Alba

doi:10.1186/s12951-019-0460-8

Cited by 35 publications

(28 citation statements)

References 72 publications

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“…The consequence was a decrease in intracellular stiffness most likely due to a disaggregation of actin filaments. 78 The resulting increase in G-actin could finally result in a decreased expression of lysyl oxidase. 44 Further studies are needed to address the expression and activity of lysyl oxidases in the C-MWCNT-supplemented ECT.…”

Section: Resultsmentioning

confidence: 99%

Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes

Kittana¹,

Assali²,

Zimmermann³

et al. 2021

IJN

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Background Under certain conditions, the physiological repair of connective tissues might fail to restore the original structure and function. Optimized engineered connective tissues (ECTs) with biophysical properties adapted to the target tissue could be used as a substitution therapy. This study aimed to investigate the effect of ECT enforcement by a complex of multiwall carbon nanotubes with chitosan (C-MWCNT) to meet in vivo demands. Materials and Methods ECTs were constructed from human foreskin fibroblasts (HFF-1) in collagen type I and enriched with the three different percentages 0.025, 0.05 and 0.1% of C-MWCNT. Characterization of the physical properties was performed by biomechanical studies using unidirectional strain. Results Supplementation with 0.025% C-MWCNT moderately increased the tissue stiffness, reflected by Young’s modulus, compared to tissues without C-MWCNT. Supplementation of ECTs with 0.1% C-MWCNT reduced tissue contraction and increased the elasticity and the extensibility, reflected by the yield point and ultimate strain, respectively. Consequently, the ECTs with 0.1% C-MWCNT showed a higher resilience and toughness as control tissues. Fluorescence tissue imaging demonstrated the longitudinal alignment of all cells independent of the condition. Conclusion Supplementation with C-MWCNT can enhance the biophysical properties of ECTs, which could be advantageous for applications in connective tissue repair.

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Section: Resultsmentioning

confidence: 99%

Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes

Kittana¹,

Assali²,

Zimmermann³

et al. 2021

IJN

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“…A pronounced drop in elastic properties has already been observed in living and fixed cells under exposure to OS. It is considered that a decrease of cell stiffness may result from changes in membrane permeability, overproduction of ROS, disruption of sites where the cytoskeleton connects to the plasma membrane, and the deterioration of the actin network [ 72 , 75 ]. Hence, the AFM results are consistent with cellular assays that indicate a myricetin-related loss of membrane integrity and death by necrosis in the presence of copper.…”

Section: Discussionmentioning

confidence: 99%

Neurotoxic Effect of Flavonol Myricetin in the Presence of Excess Copper

et al. 2021

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Oxidative stress (OS) induced by the disturbed homeostasis of metal ions is one of the pivotal factors contributing to neurodegeneration. The aim of the present study was to investigate the effects of flavonoid myricetin on copper-induced toxicity in neuroblastoma SH-SY5Y cells. As determined by the MTT method, trypan blue exclusion assay and measurement of ATP production, myricetin heightened the toxic effects of copper and exacerbated cell death. It also increased copper-induced generation of reactive oxygen species, indicating the prooxidative nature of its action. Furthermore, myricetin provoked chromatin condensation and loss of membrane integrity without caspase-3 activation, suggesting the activation of both caspase-independent programmed cell death and necrosis. At the protein level, myricetin-induced upregulation of PARP-1 and decreased expression of Bcl-2, whereas copper-induced changes in the expression of p53, p73, Bax and NME1 were not further affected by myricetin. Inhibitors of ERK1/2 and JNK kinases, protein kinase A and L-type calcium channels exacerbated the toxic effects of myricetin, indicating the involvement of intracellular signaling pathways in cell death. We also employed atomic force microscopy (AFM) to evaluate the morphological and mechanical properties of SH-SY5Y cells at the nanoscale. Consistent with the cellular and molecular methods, this biophysical approach also revealed a myricetin-induced increase in cell surface roughness and reduced elasticity. Taken together, we demonstrated the adverse effects of myricetin, pointing out that caution is required when considering powerful antioxidants for adjuvant therapy in copper-related neurodegeneration.

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“…An approximately 6-fold increase in young's modulus could be observed in the PSA-Gel-TA-15% hydrogel in comparison to the PSA-Gel hydrogel. If there is less hydrogel in the young modulus, cellular anchorage can be repelled and the encapsulated cells can expand into spheres [30].…”

Section: Mechanical Propertiesmentioning

confidence: 99%

Preparation and characterization of a novel polysialic acid/gelatin composite hydrogels cross-linked by Tannic acid to improve healing and nursing care of cesarean section dressing

Zhang¹,

Chunjie²,

Wang³

et al. 2021

Preprint

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The infections and delayed wound healing after cesarean delivery is one of the most complicated issues in surgical medicinal field. In the present investigation, designed novel polysialic acid loaded gelatin (PSA-Gel) composite hydrogels cross-linked by tannic acid (TA) has been developed and used as a facile wound dressing to improve cesarean wound healing ability with prevent bactericidal infections. The cross-linking effect was predominant when the TA content was lower, resulting in the formation of a cross-linked network. An effective TA cross-linking effect on the PSA-Gel hydrogel matrix was achieved when the amount of TA was around 15 wt %. The morphology of as-fabricated hydrogels was characterized using scanning electron microscopy (SEM) with an average pore sizes of PSA-Gel, PSA-Gel-TA-5%, PSA-Gel-TA-10%, and PSA-Gel-TA-15% hydrogels were 95.4 ± 12.6 µm, 120.4 ± 8.2 µm, 165.3 ± 21.6 µm, and 270.2 ± 32.5 µm, respectively. The effects of hydrogels on the swelling ratio, in vitro degradation, and mechanical properties were systemically evaluated. The TA cross-linked PSA-Gel hydrogels display strong antimicrobial behavior against gram-positive (Staphylococcus aureus) gram-negative (Escherichia coli) bacteria strains. Moreover, PSA-Gel-TA hydrogels also displayed favorable cytotoxicity toward L929 fibroblast cell lines. Finally, the therapeutic and wound healing potential of the PSA-Gel-TA hydrogels has been studied in vivo using the excision wound model in rats. The results indicate that the PSA-Gel-TA hydrogels have a greater and significant effect on wound closure and increased the wound healing rate compared with native PSA-Gel hydrogels and untreated control group. The findings suggest that PSA-Gel-TA hydrogels are promising dressing materials for the treatment of wound healing.

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Evaluation of the elastic Young’s modulus and cytotoxicity variations in fibroblasts exposed to carbon-based nanomaterials

Cited by 35 publications

References 72 publications

Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes

Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes

Neurotoxic Effect of Flavonol Myricetin in the Presence of Excess Copper

Preparation and characterization of a novel polysialic acid/gelatin composite hydrogels cross-linked by Tannic acid to improve healing and nursing care of cesarean section dressing

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