Evaluation of the ELVIS plate method for the detection and typing of herpes simplex virus in clinical specimens

Crist, Gwen; Langer, Janine; Woods, Gail L.; Procter, Melinda; Hillyard, David R.

doi:10.1016/j.diagmicrobio.2004.03.008

Cited by 31 publications

(10 citation statements)

References 7 publications

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“…This enzyme then cleaves a colorimetric substrate, resulting in the development of a blue color in infected cells. Positive ELVIS cultures can be detected within 24 h of inoculation, and the method has been reported to be 88 to 100% sensitive and 98 to 100% specific in a comparison with standard viral culture (Table 1) (18,(20)(21)(22).…”

Section: Methods For Detection Of Hsvmentioning

confidence: 99%

Light Microscopy, Culture, Molecular, and Serologic Methods for Detection of Herpes Simplex Virus

2014

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Herpes simplex virus 1 (HSV-1) and 2 (HSV-2) cause a variety of human diseases, ranging from acute to chronic and mild to severe. The absence of curative therapy results in lifelong carriage marked by recurrent outbreaks and allows transmission of the virus to uninfected individuals. Nonspecific lesions, variable presentation, and chronic carriage necessitate the use of different laboratory testing methods appropriate for each presentation. A thorough understanding of the performance characteristics and limitations of available tests is critical for selection of the appropriate test and interpretation of results. Clinical sensitivity, specificity, and selection of the appropriate methodology is paramount to avoid misdiagnosis and guide therapy. In this article we review the different methods for detection and typing of HSV, including light microscopy, culture, serology, and nucleic acid-based tests. We discuss the strengths and weaknesses of each method for diagnosing HSV infection, cite performance characteristics, and review appropriate clinical uses.

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Section: Methods For Detection Of Hsvmentioning

confidence: 99%

Light Microscopy, Culture, Molecular, and Serologic Methods for Detection of Herpes Simplex Virus

2014

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“…Herpes Simplex Virus Type 1 (HSV-1) is typically linked to oropharynx infections whilst HSV-2 is typically linked to neonatal or genital infections [4]. Cell culturing of lesion swab content, such as the Enzyme-Linked Virus Inducible System (ELVIS®, Diagnostics Hybrids Inc., Athens, OH) [5], are the most common way of testing HSV currently. However, cell culture-based diagnostics takes a long time (16 hours or more) for results turn-around.…”

Section: Introductionmentioning

confidence: 99%

Isothermal real-time polymerase chain reaction detection of Herpes Simplex Virus Type 1 on a light-actuated digital microfludics platform

Pei

Wang

Lin

et al. 2013

2013 Transducers &Amp; Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems

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We report on a light-actuated digital microfluidics platform for real-time, isothermal helicase-dependent polymerase chain reaction (PCR). This platform is used to detect Herpes Simplex Virus Type 1 (HSV-1). Reagents in droplets are individually addressed, mixed, transported and arrayed on-chip before the PCR process. 16 droplets were formed from mixing 400 nl PCR master mix droplets with 35 nl HSV-1 viral lysate droplets, arrayed into a 4x4 matrix, and amplified simultaneously, all on chip. Amplification and detection of HSV-1 can be achieved within 45 minutes. No cross contamination was observed.

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“…The enzyme-linked virus inducible system (ELVIS®, Diagnostics Hybrids, Inc., Athens, OH) detects HSV using transgenic shell vial culture followed by typing of HSV-1 or HSV-2 by fluorescein-labeled monoclonal antisera. 6–8 The detection limit for ELVIS® culture is estimated to be between 0.65- and 8.5-TCID50 for HSV-1 and 0.1- and 8.0-TCID50 for HSV-2 depending on the strain. 9 Compared to conventional roll tube culture, ELVIS® reduces the maximum time to detection from days to 24 hours while maintaining adequate sensitivity and eliminating subjective detection of cytopathic effect, but both methods require a cell culture facility and associated technical expertise.…”

Section: Introductionmentioning

confidence: 99%

Comparative clinical evaluation of the IsoAmp® HSV Assay with ELVIS® HSV culture/ID/typing test system for the detection of herpes simplex virus in genital and oral lesions

Miller¹,

Yen‐Lieberman²,

Poulter³

et al. 2012

Journal of Clinical Virology

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Background The novel IsoAmp® HSV Assay employs isothermal helicase-dependent nucleic acid amplification and a user-friendly disposable test device to achieve rapid (<1.5 hours), on-demand qualitative detection of herpes simplex virus (HSV) types 1 and 2 in oral and genital lesions. Objectives To compare performance of the IsoAmp® HSV Assay with the ELVIS® HSV ID/typing (shell-vial culture and DFA) test system for clinical specimens collected from oral and genital lesions in symptomatic patients. Study design A total of 994 specimens from male and female genital and oral lesions were obtained and evaluated at five study sites in the United States. Results from the IsoAmp® HSV Assay were compared to those from the ELVIS® system. Separate reproducibility studies were performed at 3 sites using a blinded and randomized study panel. Discrepant specimens were resolved by bidirectional sequencing analysis. Results After discrepant analysis, overall agreement of IsoAmp® with ELVIS® was 98.8% with 37.0% overall prevalence (all study sites). Reproducibility rates were well within expectations. Conclusion The IsoAmp® HSV assay showed excellent performance for clinical use for detection of HSV in genital and oral specimens. In contrast to ELVIS®, IsoAmp® HSV offers excellent sensitivity plus rapid on-demand testing and simpler specimen preparation.

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Evaluation of the ELVIS plate method for the detection and typing of herpes simplex virus in clinical specimens

Cited by 31 publications

References 7 publications

Light Microscopy, Culture, Molecular, and Serologic Methods for Detection of Herpes Simplex Virus

Light Microscopy, Culture, Molecular, and Serologic Methods for Detection of Herpes Simplex Virus

Isothermal real-time polymerase chain reaction detection of Herpes Simplex Virus Type 1 on a light-actuated digital microfludics platform

Comparative clinical evaluation of the IsoAmp® HSV Assay with ELVIS® HSV culture/ID/typing test system for the detection of herpes simplex virus in genital and oral lesions

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