Evaluation of the expression of TLR-2, Dectin-1 and TNF-α level in invasive aspergillosis in cancer mice

Reza, Khosravi Ali; Sohrabi, Noushin; Zuhair, Hassan; Mahdavi, Mohammad; Ali, Amini Abbas; Tebianian, Majid; Shokri, Hojjatollah; Hoseinali, Ebrahimzadeh Mousavi

doi:10.1007/s00580-009-0932-6

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…The ability of TLR2 to detect Aspergillus spores and induce autophagy is associated with its expression levels. Wu et al reported that induced expression of TLR2 by the fungus was noted in human corneal epithelial cells and mice macrophages (32,33). Consistent with these findings, in the present study, A. fumigatus exposure significantly increased the expression of TLR2 in rat alveolar cells, which was accompanied by the induction of autophagy and a decrease in the levels of miR-344b-1-3p.…”

Section: Discussionsupporting

confidence: 91%

miRNA‑344b‑1‑3p modulates the autophagy of NR8383 cells during Aspergillus�fumigatus infection via TLR2

et al. 2019

Exp Ther Med

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Autophagy serves a pivotal role in host defense during fungal infections, and the contribution by toll-like receptor 2 (TLR2) has been well demonstrated. It has been reported that microRNA-344a-1-3p (miR-344a-1-3p) can directly target TLR2. However, the expression of TLR2 significantly decreases during Aspergillus fumigatus infection. Therefore, the specific role of miR-344a-1-3p in the host defense against A. fumigatus infection remains to be elucidated. In the present study, A. fumigatus infection increased the expression of TLR2 and induced autophagy, which was indicated by increasing expression levels of autophagy-related protein 5 (ATG5), Beclin-1, light chain 3 (LC3)-1 and LC3-II, as measured by western blot analysis, and an increased number of GFP-LC3 puncta, as measured by fluorescence. Following transfection with miR-344a-1-3p mimics and/or TLR2, miR-344b-1-3p significantly inhibited the expression of TLR2, Beclin-1, ATG5, LC3-I and LC3-II, whereas the overexpression of TLR2 significantly attenuated the inhibitory effect on autophagy by miR-344b-1-3p. Collectively, these findings demonstrate that A. fumigatus can be controlled by the induction of autophagy following de-repression of the expression of TLR2, mediated by miR-344a-1-3p.

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Section: Discussionsupporting

confidence: 91%

miRNA‑344b‑1‑3p modulates the autophagy of NR8383 cells during Aspergillus�fumigatus infection via TLR2

et al. 2019

Exp Ther Med

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Structural characterization of a novel polysaccharide from Panax notoginseng residue and its immunomodulatory activity on bone marrow dendritic cells

Liu

Yang

et al. 2020

International Journal of Biological Macromolecules

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Evaluation of the expression of TLR-2, Dectin-1 and TNF-α level in invasive aspergillosis in cancer mice

Cited by 2 publications

References 22 publications

miRNA‑344b‑1‑3p modulates the autophagy of NR8383 cells during Aspergillus�fumigatus infection via TLR2

miRNA‑344b‑1‑3p modulates the autophagy of NR8383 cells during Aspergillus�fumigatus infection via TLR2

Structural characterization of a novel polysaccharide from Panax notoginseng residue and its immunomodulatory activity on bone marrow dendritic cells

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