Evaluation of the free radical scavenging activity and radioprotective efficacy ofGrewia asiaticafruit

Abstract

The radioprotective effect of Grewia asiatica fruit (GAE) which contains anthocyanin-type cyanidin 3-glucoside, vitamins C and A, minerals, carotenes and dietary fibre was studied. For the study Swiss albino mice were divided into five groups: (1) control (vehicle treated); (2) GAE treated (700 mg kg(-1) day(-1) for 15 days); (3) irradiated (5 Gy); (4) GAE+irradiated and (5) irradiated+GAE treated. The irradiation of animals resulted in a significant elevation of lipid peroxidation in terms of thiobarbituric acid reactive substances (TBARS) content and depletion in glutathione (GSH) and protein levels at all intervals studied, namely 1-30 days, in comparison to the control group. Treatment of mice with GAE before and after irradiation caused a significant depletion in TBARS content followed by a significant elevation in GSH and protein concentration in the intestine and testis of mice at all post-irradiation autopsy intervals in comparison to irradiated mice. Significant protection of DNA and RNA in testis was also noticed. GAE was found to have strong radical scavenging activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH(*)) and O(2)(-) assays and also showed in vitro radioprotective activity in protein carbonyl assay in a dose-dependent manner. The above results prove the radioprotective efficacy of GAE.

Eruca sativa and its Flavonoid Components, Quercetin and Isorhamnetin, Improve Skin Barrier Function by Activation of Peroxisome Proliferator‐Activated Receptor (PPAR)‐α and Suppression of Inflammatory Cytokines

“…The 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assay was used to determine the antioxidant capacity of ES. The DPPH radical scavenging activity is generally quantified in terms of inhibition percentage of the pre‐formed free radical by antioxidants, and the EC 50 (concentration required to obtain a 50% antioxidant effect) is a typically employed parameter to express the antioxidant capacity (Sharma and Sisodia, ).…”

Antioxidant activity and protection of pancreatic β‐cells by embelin in streptozotocin‐induced diabetes

“…Blood was analyzed for glycated hemoglobin (Hb A1C ) 14 and serum was analyzed for glucose, 15 insulin (RIA) and nitric oxide (NO) 16 . The pancreas was minced and homogenized for the analysis of superoxide dismutase (SOD), 17 catalase (CAT), 18 reduced glutathione (GSH), 19 glutathione peroxidase (GPx), 20 glutathione‐ S ‐transferase (GST), 21 lipid peroxidation (LPO), 22 and ascorbic acid 23 . For biochemical analysis, analytical grade chemicals were procured from Sigma‐Aldrich (St Louis, MO, USA) and Hi‐Media (Mumbai, India).…”

Anthocyanin‐rich Fractions of Blackberry Extracts Reduce UV‐induced Free Radicals and Oxidative Damage in Keratinocytes

“…Gingerol, another plant phenol, reduced UV‐induced ROS production and inhibited TPA‐mediated tumor promotion in mouse skin (Kim et al , ). Lastly, Grewia asciatica fruit decreased lipid peroxidation in gamma‐irradiated mice (Sharma and Sisodia, ). It is important to point out the limitations of extrapolating findings in a murine cell model to actual human skin.…”