Evaluation of the fully automated Bactec MGIT 960 system for the susceptibility testing of Mycobacterium tuberculosis to first-line drugs: a multicenter study

Kontos, Fanourios; Maniati, Matina; Costopoulos, C.; Gitti, Zoe; Nicolaou, Stavroula; Petinaki, Efymia; Anagnostou, S.; Tselentis, Ioannis; Maniatis, Antonios N.

doi:10.1016/j.mimet.2003.10.015

Cited by 32 publications

(25 citation statements)

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“…The current standard method for first-line antimicrobial sensitivity testing (AST) of M. tuberculosis in Canada is the Bactec MGIT 960 (M960) system (BD, Sparks, MD). Although the M960 system has been approved for first-line AST (2)(3)(4)20), the Bactec 460 (B460; BD, Sparks, MD) and the agar proportion method are currently used for second-line AST since the M960 system had not been validated for this purpose (3,8). Due to the increasing demand for second-line AST and the discontinuation of the B460 technology by the manufacturer, a multicenter validation of a second-line AST panel for the M960 system was undertaken.…”

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confidence: 99%

Canadian Multicenter Laboratory Study for Standardized Second-Line Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis

et al. 2011

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The purpose of this study was to establish a standardized protocol for second-line antimicrobial susceptibility testing of Mycobacterium tuberculosis using the Bactec MGIT 960 system in Canadian laboratories. Four Canadian public health laboratories compared the susceptibility testing results of 9 second-line antimicrobials between the Bactec 460 and Bactec MGIT 960 systems. Based on the data generated, we have established that the Bactec MGIT 960 system provides results comparable to those obtained with the previous Bactec 460 method. The critical concentrations established for the testing of the antimicrobials used are as follows: amikacin, 1 g/ml; capreomycin, 2.5 g/ml; ethionamide, 5 g/ml; kanamycin, 2.5 g/ml; linezolid, 1 g/ml; moxifloxacin, 0.25 g/ml; ofloxacin, 2 g/ml; p-aminosalicylic acid, 4 g/ml; rifabutin, 0.5 g/ml.The Public Health Agency of Canada (PHAC) publishes yearly statistics on the antimicrobial resistance patterns of all laboratory-isolated Mycobacterium tuberculosis strains in Canada. From 2000 to 2010, the number of M. tuberculosis isolates resistant to one or more of the first-line antimicrobials has varied between 8.0% and 11.0% of all tuberculosis (TB) cases per year in Canada (15). Between 0.9% and 1.6% of these cases were considered multidrug resistant (MDR) TB. Since 2005, TB cases in the foreign-born Canadian population have accounted for more than 60% of all Canadian TB cases and greater than 90% of MDR TB cases (10-14).Currently it is recommended that after systematic testing against first-line anti-TB agents, isolates that are found to be monoresistant to rifampin or to demonstrate resistance to any two of the first-line antimicrobials be tested against a panel of secondline antimicrobials (3,7,21). Though the majority of resistant isolates in Canada show monoresistance to isoniazid (INH), recently published Clinical and Laboratory Standards Institute (CLSI) document M24-A2 recommends that second-line antimicrobial testing also be performed on isolates that are INH monoresistant in cases where fluoroquinolones may be added to the therapy. The current standard method for first-line antimicrobial sensitivity testing (AST) of M. tuberculosis in Canada is the Bactec MGIT 960 (M960) system (BD, Sparks, MD). Although the M960 system has been approved for first-line AST (2-4, 20), the Bactec 460 (B460; BD, Sparks, MD) and the agar proportion method are currently used for second-line AST since the M960 system had not been validated for this purpose (3,8). Due to the increasing demand for second-line AST and the discontinuation of the B460 technology by the manufacturer, a multicenter validation of a second-line AST panel for the M960 system was undertaken. A validated and standardized method and antimicrobial panel for second-line AST are required to ensure that antimicrobial-resistant cases of M. tuberculosis, including MDR and extensively drug-resistant cases, are identified in an accurate and timely manner and that susceptibility testing and reporting of these isolates are standardize...

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Canadian Multicenter Laboratory Study for Standardized Second-Line Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis

et al. 2011

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“…The nonradiometric and fully automated Mycobacteria Growth Indicator Tube 960 system (MGIT 960; Becton Dickinson Microbiology Systems, Sparks, MD) has been proposed as a more efficient alternative to the semiautomated and radiometric BACTEC 460TB system (4,5). However, it is noteworthy that to date, only two prospective studies (1, 2) have examined the turnaround time (TAT) of the first-generation protocol (the manual 4-ml MGIT) for this assay with respect to both growth detection and susceptibility testing of the Mycobacterium tuberculosis complex (MTBC).…”

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Does the MGIT 960 System Improve the Turnaround Times for Growth Detection and Susceptibility Testing of the Mycobacterium tuberculosis Complex?

et al. 2006

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“…Cambau et al [1] found that the concordance between the two methods was 95.3-100% when considering the results for each drug/strain combination separately. Kontos et al [9] reported that the overall agreement between MGIT and MOP was 99.1%. High sensitivity (the ability to detect true resistance) and specificity (the ability to detect true susceptibility) values of the MGIT system were demonstrated by this study for susceptibility testing of M. tuberculosis.…”

Section: Discussionmentioning

confidence: 99%

“…All procedures in the experimental protocol were approved by the Ethics Committee of Medical Faculty. C. The criterion for resistance was 1% or more colonies in the drug-containing medium when compared to the number of colonies developing in the drug-free medium [1,3,[7][8][9]11].…”

Section: Strains and Specimensmentioning

confidence: 99%

“…Recently, the nonradiometric, fully automated Bactec MGIT 960 system (MGIT) (Becton Dickinson, Sparks, MD) technology has provided a new step forward, as it allows continuous monitoring of positive fluorescence, which is based on bacterial growth. This MGIT system has been proposed as a method for determining susceptibility of M. tuberculosis strains to the first-line antituberculosis drugs: INH, RIF, EMB and SM [4][5][6][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the Mycobacterium Growth Indicator Tube Method and the Method of Proportion for Drug Susceptibility Testing of Mycobacterium Tuberculosis

Aktaş¹,

Yiğit²,

Ayyıldız³

et al. 2014

EAJM

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Objective: Tuberculosis is an important public health problem in developed and, especially, developing countries. The incidence of multidrug-resistant Mycobacterium tuberculosis (MDR-TB) has increased in recent years. Mycobacterial culture and susceptibility testing must be rapidly concluded for effective treatment and control of the disease. The present study evaluated the reliability of the Mycobacterium Growth Indicator Tube (MGIT) method for testing the susceptibility of M. tuberculosis to four first-line antimicrobial drugs by comparing MGIT results to those obtained by the method of proportion (MOP), which served as the reference method. Materials and Methods:A total of 60 clinical isolates (28 sputum, 7 bronchoalveolar lavage, 7 cerebrospinal fluid, 3 gastric aspirates, 5 urine, 4 pleural fluid and 6 other specimens) of M. tuberculosis were tested for susceptibility to streptomycin (SM), isoniazid (INH), ethambutol (EMB) and rifampin (RIF). MOP was carried out according to National Committe for Clinical Laboratory Standards (NCCLS) on Löwenstein-Jensen medium. MGIT susceptibility testing was performed according to the protocol provided by the manufacturer. Results:Resistance was detected in 18.3% and 16.7% of the isolates for INH, 13.3% and 10.0% for RIF, 16.7% and 11.7% for SM and 6.7% and 8.3% for EMB by MOP and MGIT, respectively. Conclusion:MOP remains the method of choice, however, the correlation between MOP and MGIT suggested that MGIT can also be used routinely and that it is a reliable method for testing susceptibility of M. tuberculosis strains to first-line anti-tuberculosis drugs. Key Words: MGIT, M. tuberculosis, susceptibility ÖzetAmaç: Tuberküloz gelişmiş ve özellikle gelişmekte olan ülkeler için önemli bir halk sağlığı problemidir. Çoklu ilaç direnci gösteren Mycobacterium tuberculosis kökenlerinin sayısı son yıllarda artış göstermek-tedir. Bu hastalığın etkili tedavisi ve kontrol altına alınmasında mikrobiyolojik kültür ve duyarlılık yöntemlerinin hızla sonuçlandırılması zorunludur. Bu çalışmada, M. tuberculosis kökenlerinin birinci seçenek anti-tüberküloz ilaçlara karşı duyarlılığı Growth Indicator Tube (MGIT) ve referans metot olarak kabul edilen proporsiyon yöntemi ile araştı-rılmıştır. Gereç ve Yöntem:Değişik klinik örneklerden izole edilen 60 M. tuberculosis kökeninin (28 balgam, 7 BAL, 7 BOS, 3 açlık mide suyu, 5 idrar, 4 plevral sıvı ve 6 diğer örnekler) dört birinci seçenek anti-tüberküloz ilaca (streptomisin (SM), izoniazid (INH), etambutol (EMB) and rifampisin (RIF)) karşı duyarlılığı araştırıldı. Proporsiyon metodu NCCLS standartlarına göre Löwenstein-Jensen besiyerinde uygulandı. MGIT sistemi ile duyarlılık üretici firma önerileri doğrultusunda çalışıldı. Bulgular: İki yöntem ile yapılan duyarlılık çalışmasında kökenlerin ilaçlara karşı duyarlılığı iki yöntem (MOP ve MGIT) için sırasıyla; INH direnci %18,3 ve %16,7, RIF direnci %13,3 ve %10,0, SM direnci %16,7 ve%11,7, EMB direnci %6,7 ve %8,3 olarak belirlendi.Sonuç: Proporsiyon metodu standart yöntem olmakla birlikte...

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Evaluation of the fully automated Bactec MGIT 960 system for the susceptibility testing of Mycobacterium tuberculosis to first-line drugs: a multicenter study

Cited by 32 publications

References 6 publications

Canadian Multicenter Laboratory Study for Standardized Second-Line Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis

Canadian Multicenter Laboratory Study for Standardized Second-Line Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis

Does the MGIT 960 System Improve the Turnaround Times for Growth Detection and Susceptibility Testing of the Mycobacterium tuberculosis Complex?

Comparison of the Mycobacterium Growth Indicator Tube Method and the Method of Proportion for Drug Susceptibility Testing of Mycobacterium Tuberculosis

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