The management of effluents and their treatment is a fundamental issue in water management, the removal of different types of contaminants is another relevant issue for public health and the environment. Bacteria are one of the main types of contaminants in untreated water discharged to receiving bodies. The objective of this research was to evaluate the removal efficiency of pathogenic bacteria in a horizontal feeding subsurface artificial wetland that treats wastewater originated from the Boca de Río Technological Institute, Veracruz, Mexico. A hybrid system composed of seven cells with three types of substrates and ornamental type vegetation was designed; the indicators evaluated were the concentration of total and fecal coliforms and the efficiency of bacterial removal in the stages of the system. The artificial wetland system demonstrated a significant reduction (p < 0.05) between the different cells of the system. The values of pathogenic bacteria removal obtained in the wetland were higher than 99% in the cells of the system and times. In conclusion, it was identified that the interaction of the components of this system and its operation under the climatic seasons of the site influenced the removal efficiency of pathogenic bacteria, allowing optimal removal efficiency.