This paper study investigates the role and impact of e-scooters in urban mobility within Thessaloniki, Greece, focusing on usage patterns, user demographics, and the socio-economic and environmental implications. A mixed-methods approach, incorporating both quantitative data from user surveys and qualitative insights from interviews, was employed to develop a structured Google Forms questionnaire that was disseminated to e-scooter users in Thessaloniki using a modified snowball sampling technique. The survey revealed that the primary users are young adults aged 18-27, predominantly students, who favor e-scooters for their time efficiency, enjoyment, and environmental benefits. However, safety concerns and inadequate infrastructure pose significant barriers to wider adoption. The findings highlight strong public support for expanding the e-scooter network to enhance connectivity and convenience. Recommendations include developing dedicated infrastructure, implementing balanced regulatory measures, maintaining affordability, and promoting public awareness campaigns. Future research should explore the integration of e-scooters with other public transport modes and assess their long-term impact on urban mobility and sustainability. This study contributes valuable insights for policymakers aiming to develop sustainable urban transport systems that meet evolving urban needs.