Evaluation of the genetic diversity and population structure of sesame (Sesamum indicum L.) using microsatellite markers

Cho, Young-Il; Park, Jong‐Hyun; Lee, Chul-Won; Ra, Won-Hee; Chung, Jong‐Wook; Lee, Jung-Ro; Ma, Kyung‐Ho; Lee, Seok-Young; Lee, Kang-Seob; Lee, Myung Chul; Park, Yong‐Jin

doi:10.1007/s13258-010-0130-6

Cited by 47 publications

(46 citation statements)

References 33 publications

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“…The higher PIC value for SSR has been reported earlier in common bean (Zargar et al 2016) and walnut (Ahmed et al 2012). The PIC values ranging from 0.03 to 0.96 were reported in the previous studies also (Li-Bin et al 2008; Cho et al 2011; Spandana et al 2012; Park et al 2013). …”

Section: Discussionsupporting

confidence: 66%

“…The molecular techniques of RAPD (Bhat et al 1999; Ercan et al 2004; Kumar and Sharma 2009; Pham et al 2011; Akbar et al 2011; Mahdizadeh et al 2012), AFLP (amplified fragment length polymorphism) (Laurentin and Karlovsky 2006, 2007), ISSR (inter-simple sequence repeats) (Kim et al 2002; Parsaeian et al 2011; Kumar and Sharma 2011; Nyongesa et al 2013; Woldesenbet et al 2015), SSR (Spandana et al 2012; Dixit et al 2005; Cho et al 2011; Wang et al 2012; Yepuri et al 2013; Wei et al 2014; Surapaneni et al 2014; Uncu et al 2015; Dossa et al 2016; Sehr et al 2016), and SRAP (sequence-related amplified polymorphism) (Li and Quiros 2001; Li et al 2007; Zhang et al 2010) have been used to evaluate the genetic diversity in sesame of different origins. Morphologically, Indian collection of sesame is characterized by variable forms being cultivated in different agroclimatic zones.…”

Section: Introductionmentioning

confidence: 99%

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Comparative assessment of genetic diversity in Sesamum indicum L. using RAPD and SSR markers

et al. 2017

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Sesame (Sesamum indicum L.) is an ancient oilseed crop known for its nutty seeds and high-quality edible oil. It is an unexplored crop with a great economic potential. The present study deals with assessment of genetic diversity in the crop. Twenty two RAPD and 18 SSR primers were used for analysis of the 47 different sesame accessions grown in different agroclimatic zones of India. A total of 256 bands were obtained with RAPD primers, of which 191 were polymorphic. SSR primers gave 64 DNA bands, of which all of were polymorphic. The Jaccardʼs similarity coefficient of RAPD, SSR, and pooled RAPD and SSR data ranged from 0.510 to 0.885, 0.167 to 0.867, and 0.505 to 0.853, respectively. Maximum polymorphic information content was reported with SSRs (0.194) compared to RAPDs (0.186). Higher marker index was observed with RAPDs (1.426) than with SSRs (0.621). Similarly, maximum resolving power was found with RAPD (4.012) primers than with SSRs (0.884). The RAPD primer RPI-B11 and SSR primer S16 were the most informative in terms of describing genetic variability among the varieties under study. At a molecular level, the seed coat colour was distinguishable by the presence and absence of a group of marker amplicon/s. White and brown seeded varieties clustered close to each other, while black seeded varieties remained distanced from the cluster. In the present study, we found higher variability in Sesamum indicum L. using RAPD and SSR markers and these could assist in DNA finger printing, conservation of germplasm, and crop improvement.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0578-4) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Comparative assessment of genetic diversity in Sesamum indicum L. using RAPD and SSR markers

et al. 2017

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“…Several researchers have performed diversity and population structure analyses using various DNA marker techniques in different plants such as wheat (Chen et al, 2012), common bean (Asfaw et al, 2009), sesame (Cho et al, 2011), sunflower (Mandel et al, 2011), rice (Ming et al, 2010), and maize (Stich et al, 2010). The model-based structure analysis used herein revealed the presence of 2 populations among the collected genotypes.…”

Section: Discussionmentioning

confidence: 99%

The genus Crocus, series Crocus (Iridaceae) in Turkey and 2 East Aegean islands: a genetic approach

Erol¹,

Kaya²,

Şık³

et al. 2014

Turk J Biol

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In this study, a total of 26 Crocus specimens from different locations across Turkey and 2 East Aegean islands (Chios and Samos) were analyzed using 12 amplified fragment length polymorphism (AFLP) primer combinations to obtain information on genetic diversity, population structure, and genetic relationships. A total of 369 polymorphic AFLP bands were generated and scored as binary data. Genetic similarities were determined. Cluster analysis revealed 4 major groups among the 26 genotypes examined in this study. The nuclear DNA contents (2C) of the 26 Crocus specimens were found to range from 5.08 pg in C. asumaniae to 9.75 pg in C. sativus. Polymorphic information content (PIC) values were used to examine the capacity of the various primer pairs to amplify polymorphisms in the Crocus specimens. The PIC values ranged from 0.218 (M-CAA/E-AGC) to 0.512 (M-CAT/E-AAG) and showed an average of 0.34. In sum, we herein used AFLP analysis to identify a high level of polymorphism among Crocus specimens collected from various locations in Turkey and Greece, and our structural analysis yielded 2 reconstructed populations. These findings provide new insight into the relationships among different Crocus genotypes and show that AFLP analysis can be useful for Crocus diversity studies.

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“…However, it is difficult to decide as to when to harvest a sesame crop to maximize the yield, because plant growth is originally indeterminate and capsules dehisce when mature (Day, 2000). They have been used to investigate genetic diversity and phylogenetic relationships in sesame (Cho et al, 2011;Vinod and Sharma, 2011;Zhang H et al, 2012;Zhang Y et al, 2012;Nyongesa et al, 2013). Determinate growth habit sesame offers a solution to such difficulties and is now a prerequisite for adapting sesame to modern farming systems.…”

Section: Introductionmentioning

confidence: 99%

Assessment of molecular genetic diversity and population structure of sesame (Sesamum indicumL.) core collection accessions using simple sequence repeat markers

et al. 2013

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Sesame (Sesamum indicum L.) is one of the oldest oil crops and is widely cultivated in Asia andAfrica. The aim of this study was to assess the genetic diversity, phylogenetic relationships and population structure of 277 sesame core collection accessions collected from 15 countries in four different continents. A total of 158 alleles were detected among the sesame accessions, with the number varying from 3 to 25 alleles per locus and an average of 11.3. Polymorphism information content values ranged from 0.34 to 0.84, with an average of 0.568. These values indicated a high genetic diversity at 14 loci both among and within the populations. Of these, 44 genotypespecific alleles were identified in 12 of the 14 polymorphic simple sequence repeat markers. The core collection preserved a much higher level of genetic variation. Therefore, 10.1% was selected as the best sampling percentage from the whole collection when constructing the core collection. The 277 core collection accessions formed four robust clusters in the unweighted pair group method and the arithmetic averages (UPGMA) dendrogram, although the clustering did not indicate any clear division among the sesame accessions based on their geographical locations. Similar patterns were obtained using model-based structure analysis and countrybased dendrograms, as some accessions situated geographically far apart were grouped together in the same cluster. The results of these analyses will increase our understanding of the genotypespecific alleles, genetic diversity and population structure of core collections, and the information can be used for the development of a future breeding strategy to improve sesame yield.

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Evaluation of the genetic diversity and population structure of sesame (Sesamum indicum L.) using microsatellite markers

Cited by 47 publications

References 33 publications

Comparative assessment of genetic diversity in Sesamum indicum L. using RAPD and SSR markers

Comparative assessment of genetic diversity in Sesamum indicum L. using RAPD and SSR markers

The genus Crocus, series Crocus (Iridaceae) in Turkey and 2 East Aegean islands: a genetic approach

Assessment of molecular genetic diversity and population structure of sesame (Sesamum indicumL.) core collection accessions using simple sequence repeat markers

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