Evaluation of the genetic profiles of Brucella melitensis strain from Turkey using multilocus variable number tandem repeat analysis (MLVA) and multilocus sequence typing (MLST) techniques

Akar, Kadir; Erganiş, Osman

doi:10.1016/j.vetmic.2022.109423

Cited by 13 publications

(4 citation statements)

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“…B. melitensis has been the dominant circulating population for nearly eight decades, responsible for the majority of human brucellosis cases. A similar high prevalence of B. melitensis has been observed in small ruminants in Turkey, as evidenced by isolations from sheep, goat, and cattle abortion samples from various farms across seven geographical regions (Akar and Erganis, 2022). Furthermore, Brucella strains isolated from all regions of Kyrgyzstan were identified as B. melitensis (Kydyshov et al, 2022).…”

Section: Discussionsupporting

confidence: 68%

Epidemiological changes and molecular characteristics of Brucella strains in Ningxia, China

Liu,

Ma,

Zhang

et al. 2024

Front. Microbiol.

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ObjectiveHuman brucellosis causes serious public health concerns in Ningxia, China.MethodsThis study employed epidemiological, bacteriological, and multiple-locus variable-number tandem repeat analysis (MLVA) methods to conduct an epidemiological investigation, which is necessary for devising tailored control strategies.ResultsBetween 1958 and 2022, 29,892 cases were reported, with an average annual number of cases and incidence of 467 and 7.1/100,000, respectively. The epidemic situation gradually worsened, with cases escalating from 26 cases in 2005 to 6,292 in 2022, with the incidence rate rising from 0.441 in 2005 to 86.83 in 2022. Geographically, the disease spread from a single affected county in 2004 to encompass all 22 counties in 2022. Yanchi County had the highest incidence, followed by the Hongsibao and Tongxin counties. These data suggest that Brucella infection has become a rampant regional concern in human brucellosis. Between 1958 and 2019, a total of 230 Brucella strains were identified across four studied hosts. These strains comprised four species with 12 biovars, including B. melitensis bv. 1, bv. 2, bv. 3, B. abortus bv. 1, bv. 3, bv. 4, bv. 5, bv. 6, bv. 7, B. suis bv. 1 and bv. 3, and B. canis. These data highlight the high species/biovars and host diversity of the Brucella population, posing a substantial challenge to brucellosis surveillance. There was an apparent transition from multiple species/biovars historically to the current dominance of a single species, B. melitensis, emphasizing the requirement for strengthening surveillance of B. melitensis. Genotypes 42 and 116, constituting 96.2% of the total number of genotypes, predominated in panel 1 and MLVA-11, indicating that all strains belong to the East Mediterranean lineage. MLVA cluster analysis revealed persistent transmission of dominant circulating genotypes, presenting an epidemic pattern characterized primarily by epidemiologically related cases with a few sporadic cases. Strains in this study exhibited high genetic homogeneity with strains from the Northwest, and those from Kazakhstan and Mongolia.ConclusionThe epidemic situation of human brucellosis has gradually worsened; the rampant epidemic of the disease has become a regional concern. The present study highlights that implementing the of targeted surveillance and intervention strategies is urge.

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Section: Discussionsupporting

confidence: 68%

Epidemiological changes and molecular characteristics of Brucella strains in Ningxia, China

Liu,

Ma,

Zhang

et al. 2024

Front. Microbiol.

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“…The Ministry of Agriculture and Forestry in Türkiye initiated a conjunctival mass vaccination program in 2012, aiming to combat a specific disease. Data from the Turkish Ministry of Health's official page, spanning from 2008 to 2017, provides valuable insights into the effectiveness of this vaccination effort on the number of human cases [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Human brucellosis and associated risk factors in the Middle East region: A comprehensive systematic review, meta-analysis, and meta-regression

Dadar,

Al-Khaza'leh,

Fakhri

et al. 2024

Heliyon

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“…ST8 is the main prevalent genotype in Guizhou Province and China (Ma et al, 2016;Tian et al, 2017b;Cao et al, 2018b). It is widely distributed in Asia, Europe, and Africa, and it is also a popular genotype in foreign countries (Shome et al, 2016;Akar and Erganis, 2022;Dadar et al, 2022). ST2 of B. abortus in Guizhou Province is also the genotype of B. abortus widely epidemic in China (Piao et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiological characteristics of Brucella in Guizhou Province, China, from 2009 to 2021

et al. 2023

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IntroductionBrucellosis was made statutorily notifiable in 1955, in China, while in Guizhou Province, the pathogen of human brucellosis was isolated for the first time in 2011. However, currently, the brucellosis epidemic is becoming more and more severe in Guizhou Province. The type distribution and genetic characteristics of Brucella in Guizhou Province, as well as its evolutionary relationship with domestic and foreign strains, are still unclear.MethodsMLST, MLVA, and rpoB typing techniques were used for the molecular epidemiological study of the 83 Brucella isolates in Guizhou province.ResultsAmong the 83 Brucella strains, MLST identified three ST genotypes, of which ST39 is a newly reported type in China. MLVA-16 generated 49 genotypes, and MLVA-11 generated 5 known genotypes and 2 unreported genotypes. Six genotypes were identified by rpoB technology.DiscussionMLVA has a high resolution, but differences at the Bruce 04 and 16 loci cannot exclude associations between epidemics, and combining MLST and rpoB typing methods for epidemiologic tracing can avoid erroneous judgments. Moreover, through the combined analysis of the three typing techniques, the possible origin of the new Brucella can be reasonably inferred, which is also conducive to promoting the subsequent research of the novel Brucella.

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Evaluation of the genetic profiles of Brucella melitensis strain from Turkey using multilocus variable number tandem repeat analysis (MLVA) and multilocus sequence typing (MLST) techniques

Cited by 13 publications

References 28 publications

Epidemiological changes and molecular characteristics of Brucella strains in Ningxia, China

Epidemiological changes and molecular characteristics of Brucella strains in Ningxia, China

Human brucellosis and associated risk factors in the Middle East region: A comprehensive systematic review, meta-analysis, and meta-regression

Molecular epidemiological characteristics of Brucella in Guizhou Province, China, from 2009 to 2021

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