Evaluation of the genotoxic potential of standardized extract of Glycyrrhiza glabra (GutGard™)

Chandrasekaran, C.V.; Sundarajan, K.; Gupta, Anumita; Srikanth, H; Edwin, Jothie Richard; Agarwal, Amit

doi:10.1016/j.yrtph.2011.10.002

Cited by 32 publications

(27 citation statements)

References 28 publications

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“…In the micronucleus test, no statistically significant increase in the frequency of cells with micronucleus was observed upon treatment with NR-INF-02 at the indicated concentrations (250.36 μ g/mL, 791.14 μ g/mL and 2500 μ g/mL), in the presence and absence of S9, in both short (4 h) and long term (18 h) exposure cultures. Vehicle control had an insignificant number of micronucleus which were within the limit of published data [28, 30, 31]. As evident, the positive controls mitomycin C and benzo[a]pyrene formed significant ( P < 0.05) increase in the frequency of cells with micronucleus in absence and presence of S9 respectively.…”

Section: Resultssupporting

confidence: 69%

“…Exposure of NR-INF-02 at the indicated concentrations (250.36 μ g/mL, 791.14 μ g/mL and 2500.0 μ g/mL) to human lymphocytes did not induce a statistically significant increase in the number of cells with chromosome aberrations in the absence and presence of S9 in both short and long term exposure studies. As determined by ANOVA followed by Dunnett's multiple comparison tests, vehicle control cultures had an insignificant number of structural chromosomal aberrations which were within the limit of published data [28, 30, 31]. The positive controls, mitomycin C, and benzo[a]pyrene were found to induce statistically significant ( P < 0.05) number of structural chromosomal aberrations, namely, chromosome break, chromatid break, deletion in absence and presence of S9 respectively in human peripheral blood lymphocytes (Tables 3, 4 and 5).…”

Section: Resultsmentioning

confidence: 50%

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Safety Evaluation of Turmeric Polysaccharide Extract: Assessment of Mutagenicity and Acute Oral Toxicity

Velusami

Boddapati

Srinivasa

et al. 2013

BioMed Research International

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Curcuma longa Linn. (Zingiberaceae) commonly known as turmeric has long been used for centuries as a spice and household remedy. The present study was carried out to assess the possible mutagenic potential and acute oral toxicity of polysaccharide extract of turmeric rhizome (NR-INF-02) using standard tests. The standard battery of in vitro genotoxicity tests, bacterial reverse mutation test (BRMT), chromosome aberration (CA), and micronucleus (MN) tests were employed to assess the possible mutagenic activity of NR-INF-02 (Turmacin). The results showed no mutagenic effect with NR-INF-02 up to a dose of 5000 µg/mL in BRMT. The results on CA and MN tests revealed the non clastogenic activity of NR-INF-02 in a dose range of 250.36 to 2500 µg/mL with and without metabolic activation (S9). In acute oral toxicity study, NR-INF-02 was found to be safe up to 5 g/kg body weight in Wistar rats. Overall, results indicated that polysaccharide extract of C. longa was found to be genotoxically safe and also exhibited maximum tolerable dose of more than 5 g/kg rat body weight.

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Section: Resultssupporting

confidence: 69%

Section: Resultsmentioning

confidence: 50%

Safety Evaluation of Turmeric Polysaccharide Extract: Assessment of Mutagenicity and Acute Oral Toxicity

Velusami

Boddapati

Srinivasa

et al. 2013

BioMed Research International

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“…The genotoxic potential of G. glabra was investigated using the Ames II TM , chromosomal aberration, and micronucleus test systems, and the results indicated that the herb possessed no mutagenic activities, either with or without metabolic activation (63,64). Martinez et al (65) reported that TA98 was more sensitive to the mutagens present in the licorice water extract.…”

Section: Discussionmentioning

confidence: 99%

Toxic potentials of ten herbs commonly used for aphrodisiac effect in Turkey

Asadi¹,

Nath²,

Özhan³

2015

Turk J Med Sci

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IntroductionSince ancient times, herbs have played an important role in the treatment of different diseases in many regions of world, largely for their expectorant, aphrodisiac, diuretic, diaphoretic, antispasmodic, stomachic, and sedative properties (1). The World Health Organization reported that 70%-80% of the global population relies on herbs for primary health care. In addition, because they are natural products, it is generally believed that herbs are essentially safe and free from side effects. Consequently, the consumption of nonprescription herbs has steadily increased over the past few decades (2).Despite the therapeutic advantages of herbs, reports have indicated the potential toxicity of some herbal components. Herbs contain a variety of chemical substances that act upon the body. In addition, the quality of herbs can be affected by toxic contaminants originating from industry, agriculture, and private households, including toxins that can cause mutagenic and carcinogenic effects from long-term and widespread use (3-6). Moreover, herbal medicines are generally a mixture of a number of herbs in a single preparation and contain several active ingredients that induce various pharmacological effects (7,8). To date, there are few scientific studies on the safety and potential toxicity of herbs, despite growing concerns over the lack of both scientific evidence and quality control data regarding the safety and efficacy of herbs (9,10).Sexual dysfunction is a condition that affects 15-30 million men worldwide, and it occurs in 10%-52% of men and 25%-63% of women (11,12). Many believe that sexual dysfunction is associated with the modern lifestyle. As a result, individuals turn to natural products, such as traditional herbs, that produce aphrodisiac effects for sexual enhancement. Aphrodisiac herbs are used to treat sexual dysfunction because they alter specific neurotransmitters or sex hormones. Specifically, herbs with aphrodisiac properties can induce vasodilatation that results in a sustained erection, and can cause irritation of genital mucosa that enhances sensory experience during coitus (13).Background/aim: Sexual dysfunction is a serious problem worldwide. In Turkey, herbal products are used by some people suffering from sexual dysfunction. Despite their therapeutic advantages, some constituents of herbs are potentially toxic and pose health risks because they can be bought from the market without a prescription. Therefore, we aimed to determine the safety of herbs possessing aphrodisiac effects, chosen on the basis of their frequency of medicinal use and commercial importance in Turkey. Materials and methods:Ten herbs (Anethum graveolens, Carthamus tinctorius, Citrus aurantium, Cocos nucifera, Glycyrrhiza glabra, Melissa officinalis, Nigella arvensis, Pinus pinea, Prunus mahaleb, and Zingiber officinale) were extracted with water, methanol, and chloroform. The cyto-and genotoxic potentials of the extracts were assessed using an MTT test on a rat kidney cell line and an Ames assay in Salmonella typhi...

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“…Salmonella typhimurium strains, TA98 (frameshift mutations) and TA100 (base-pair substitutions), are used in this assay. Concentration range of the compounds was between 16 and 5000 mg/mL according to the previous guidelines 55 . Compounds were prepared in six different concentrations (5, 2.5, 1.25, 0.625, 0.3125 and 0.156 mg/mL) in DMSO.…”

Section: Genotoxicity Testmentioning

confidence: 99%

Design, synthesis, and evaluation of novel 2-phenylpropionic acid derivatives as dual COX inhibitory-antibacterial agents

Gençer

Çevik

Çavuşoğlu

et al. 2017

Journal of Enzyme Inhibition and Medicinal Chemistry

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A series of 2-(4-substitutedmethylphenyl)propionic acid derivatives (6a-6m) were synthesized, characterized and evaluated for cyclooxygenase (COX) enzyme inhibitory and antimicrobial activity. Test compounds that exhibited good COX inhibition and antibacterial activity were further screened for their cytotoxicity and genotoxicity. Compounds 6h and 6l showed better COX-1 and COX-2 inhibition when compared to ibuprofen. Inhibition potency of these compounds against COX-2 was very close to that of nimesulide. The compounds 6d, 6h, 6l and 6m displayed promising antibacterial property when compared to chloramphenicol. However, the compound 6l was emerged as the best dual COX inhibitory-antibacterial agent in this study. The ADME prediction of the compounds revealed that they may have a good pharmacokinetic profile. Docking results of the compounds 6h and 6l with COX-1 (PDB ID: 1EQG) also exhibited a strong binding profile. ARTICLE HISTORY

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Evaluation of the genotoxic potential of standardized extract of Glycyrrhiza glabra (GutGard™)

Cited by 32 publications

References 28 publications

Safety Evaluation of Turmeric Polysaccharide Extract: Assessment of Mutagenicity and Acute Oral Toxicity

Safety Evaluation of Turmeric Polysaccharide Extract: Assessment of Mutagenicity and Acute Oral Toxicity

Toxic potentials of ten herbs commonly used for aphrodisiac effect in Turkey

Design, synthesis, and evaluation of novel 2-phenylpropionic acid derivatives as dual COX inhibitory-antibacterial agents

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