Evaluation of the HerpeSelect Express Rapid Test in the Detection of Herpes Simplex Virus Type 2 Antibodies in Patients With Genital Ulcer Disease

Al‐Shobaili, Hani A.; Hassanein, Khaled; Mostafa, Mohammed; Duways, Ali Saleh Al

doi:10.1002/jcla.21725

Cited by 6 publications

(2 citation statements)

References 22 publications

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“…Although the search was completed on December 31, 2018, there may be other 2018 studies that were yet to be published on the day of the search. Sample sizes within studies ranged from 60 in Al‐Shobaili et al [24] and Loughman et al [25] to 3408 in Patel et al [26] with a median sample size being of 179 patients (Table 1). Studies investigated diagnostic tools for HSV-1/2 (n=10), HSV-2 only (n=7), or HSV-1 only (n=2), and in 1 study, the viral subtype could not be ascertained [27], so it was labelled as HSV-1/2.…”

Section: Resultsmentioning

confidence: 99%

Tools for the Diagnosis of Herpes Simplex Virus 1/2: Systematic Review of Studies Published Between 2012 and 2018

Arshad

Alturkistani

Brindley

et al. 2019

JMIR Public Health Surveill

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Background Herpes simplex virus (HSV)-1 and HSV-2 are common infections affecting the global population, with HSV-1 estimated to affect 67% of the global population. HSV can have rare but severe manifestations, such as encephalitis and neonatal herpes, necessitating the use of reliable and accurate diagnostic tools for the detection of the viruses. Currently used HSV diagnostic tools require highly specialized skills and availability of a laboratory setting but may lack sensitivity. The numerous recently developed HSV diagnostic tools need to be identified and compared in a systematic way to make the best decision about which diagnostic tool to use. The diagnosis of HSV is essential for prompt treatment with antivirals. To select the best test for a patient, knowledge of the performance and limitations of each test is critical. Objective This systematic review has summarized recent studies evaluating HSV-1 and HSV-2 diagnostic tools. Methods Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, selection criteria, data extraction, and data analysis were determined before the commencement of the study. Studies assessing the specificity/sensitivity of HSV-1 or HSV-2 diagnostic tools published between 2012 and 2018 were included. Quality assessment of included studies was performed using the quality assessment of diagnostic accuracy studies (QUADAS-2) tool. Results Searches of the PubMed database yielded 264 studies; 11 studies included 11 molecular assays, and 8 studies included 19 different serological assays for the detection of HSV-1, HSV-2, or both. A greater proportion of molecular assay–based tools are being developed by commercial entities. Studies that tested molecular assays mostly focused on cutaneous and mucosal HSV infections (n=13); 2 studies focused on ocular disease, whereas only 1 study focused on the central nervous system manifestations. The Simplexa HSV 1 & 2 Direct is currently the only Food and Drug Administration–approved device for use on cerebrospinal fluid. No tools focused on prenatal screening. We also present performance metrics of tests for benchmarking of future technology. Most of the included studies had a high risk of bias rating in half of the QUADAS-2 tool risk of bias domains. Conclusions The use of serologic tests to diagnose genital lesions is inappropriate because positive results may be due to chronic infection, whereas negative results may overlook recent infection. The incidence of acute infections is rising. As these infections present the greatest risk to fetuses, work needs to be done to prevent vertical transfer. Prenatal screening for primary infection and subsequent medical intervention will assist in lowering the rate of neonatal herpes. In conclusion, HSV diagnosis is moving away from culture-based methods to serology-based or polymerase chain reaction–based methods. Sensitive, rapid, and efficien...

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Section: Resultsmentioning

confidence: 99%

Tools for the Diagnosis of Herpes Simplex Virus 1/2: Systematic Review of Studies Published Between 2012 and 2018

Arshad

Alturkistani

Brindley

et al. 2019

JMIR Public Health Surveill

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show abstract

“…The POC tests are usually based on microfluidic platforms [ 24 ] and employ tests based on ELISA, immunofluorescence, PCR, and LAMP. Several such kits are commercially available [ 38 , 39 , 40 , 41 ]. The POC devices not only enable rapid and early HSV detection but are also capable of specifically differentiating the type of virus (HSV-1 vs. HSV-2).…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Nath

Kabir

Doust

et al. 2021

Infectious Disease Reports

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Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages.

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Sexually Transmitted Diseases in Females

Garg¹,

Maheshwari²

2022

Skin Diseases in Females

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Evaluation of the HerpeSelect Express Rapid Test in the Detection of Herpes Simplex Virus Type 2 Antibodies in Patients With Genital Ulcer Disease

Abstract: The HerpeSelect Express rapid test has adequate sensitivity and specificity for confirming HSV-2 infection in patients with GUD, indicating its suitability for epidemiological studies.

Cited by 6 publications

References 22 publications

Tools for the Diagnosis of Herpes Simplex Virus 1/2: Systematic Review of Studies Published Between 2012 and 2018

Tools for the Diagnosis of Herpes Simplex Virus 1/2: Systematic Review of Studies Published Between 2012 and 2018

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Sexually Transmitted Diseases in Females

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