Evaluation of the <i>Mycoplasma genitalium</i> Resistance Plus kit for the detection of <i>M. genitalium</i> and mutations associated with macrolide resistance

Pitt, Rachel; Cole, Michelle; Fifer, Helen; Woodford, Neil

doi:10.1136/sextrans-2017-053366

Cited by 19 publications

(8 citation statements)

References 10 publications

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“…Therefore, false-negative results from the resistance PCR assay may account for some failures of azithromycin in cases thought to be macrolide susceptible. Published evaluations of the ResistancePlus assay have varied in their methods, likely explaining some variation in reported sensitivity, but false-negative results do occur [21, 26, 27]. Clinicians should be aware of this limitation, particularly when confronted by unexpectedly macrolide-susceptible cases such as after azithromycin treatment failure.…”

Section: Discussionmentioning

confidence: 99%

Outcomes of Resistance-guided Sequential Treatment ofMycoplasma genitaliumInfections: A Prospective Evaluation

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Fairley

Murray

et al. 2018

Clinical Infectious Diseases

168

139

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Section: Discussionmentioning

confidence: 99%

Outcomes of Resistance-guided Sequential Treatment ofMycoplasma genitaliumInfections: A Prospective Evaluation

Read

Fairley

Murray

et al. 2018

Clinical Infectious Diseases

168

139

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“…[ 7 , 10 ] New tests including detection of azithromycin resistance in MG were recently CE labeled. [ 11 , 12 ]…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Mycoplasma genitalium in men with urethritis in a large public hospital in Brussels, Belgium: An observational, cross-sectional study

Libois

Hallin²,

Crucitti

et al. 2018

PLoS ONE

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BackgroundMycoplasma genitalium (MG) is a cause of urethritis. While resistance to azithromycin is increasing, routine detection of MG is not performed in Belgium, where its prevalence is unknown. The aim of this study is to determine prevalence of MG in men with urethritis.Method and findingsAn “in-house” amplification assay detecting MG was performed on urine of men with complaints of urethritis who consulted the emergency unit or the Sexually Transmitted Infection clinic of our public hospital in Brussels. Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) were tested on the same sample. A total of 187 men were tested. Prevalence of MG was 9% (95% Confidence Interval: 5 to 13.2%). CT was detected in 20%, NG in 22% and 56% of samples were negative for these three pathogens. Neither age, ethnic origin, sexual orientation nor HIV infection were associated with MG urethritis.ConclusionM. genitalium was identified in 9% of men with complaints of urethritis indicating that amplification assay detecting MG should be implemented in routine testing for those patients.

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“…Antibiotic resistance screening. Mutations (A2058G, A2058C, A2058T, A2059G, and A2059C) within the macrolide resistance-determining region (MRDR) of the 23S rRNA gene were detected using a commercially available kit (the ResistancePlus MG kit; SpeeDx, Sydney, Australia) (21)(22)(23). The kit is TGA approved and CE-IVD marked; however, it is not currently for sale in the United States.…”

mentioning

confidence: 99%

Levels of Mycoplasma genitalium Antimicrobial Resistance Differ by Both Region and Gender in the State of Queensland, Australia: Implications for Treatment Guidelines

Sweeney

Trembizki

Bletchly³

et al. 2019

J Clin Microbiol

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Mycoplasma genitaliumis frequently associated with urogenital and rectal infections, with the number of cases of macrolide-resistant and quinolone-resistantM. genitaliuminfection continuing to increase. In this study, we examined the levels of resistance to these two common antibiotic treatments in geographically distinct locations in Queensland, Australia. Samples were screened for macrolide resistance-associated mutations using a commercially available kit (ResistancePlus MG; SpeeDx), and quinolone resistance-associated mutations were identified by PCR and DNA sequencing. Comparisons between antibiotic resistance mutations and location/gender were performed. The levels ofM. genitaliummacrolide resistance were high across both locations (62%). Quinolone resistance mutations were found in ∼10% of all samples, with a number of samples harboring mutations conferring resistance to both macrolides and quinolones. Quinolone resistance was higher in southeast Queensland than in north Queensland, and this was consistent in both males and females (P = 0.007). TheM. genitaliumisolates in rectal swab samples from males harbored high levels of macrolide (75.9%) and quinolone (19%) resistance, with 15.5% harboring resistance to both classes of antibiotics. Overall, the lowest observed level of resistance was to quinolones in females from north Queensland (1.6%). These data highlight the high levels of antibiotic resistance inM. genitaliumisolates within Queensland and the challenges faced by sexually transmitted infection clinicians in managing these infections. The data do, however, show that the levels of antibiotic resistance may differ between populations within the same state, which has implications for clinical management and treatment guidelines. These findings also support the need for ongoing antibiotic resistance surveillance and tailored treatment.

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Evaluation of the Mycoplasma genitalium Resistance Plus kit for the detection of M. genitalium and mutations associated with macrolide resistance

Cited by 19 publications

References 10 publications

Outcomes of Resistance-guided Sequential Treatment ofMycoplasma genitaliumInfections: A Prospective Evaluation

Outcomes of Resistance-guided Sequential Treatment ofMycoplasma genitaliumInfections: A Prospective Evaluation

Prevalence of Mycoplasma genitalium in men with urethritis in a large public hospital in Brussels, Belgium: An observational, cross-sectional study

Levels of Mycoplasma genitalium Antimicrobial Resistance Differ by Both Region and Gender in the State of Queensland, Australia: Implications for Treatment Guidelines

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