Evaluation of the immune response in Shitou geese (<i>Anser anser domesticus</i>) following immunization with GPV-VP1 DNA-based and live attenuated vaccines

Deng, Shihai; Cai, Mingsheng; Cui, Wei; Huang, Jinlu; Li, Meili

doi:10.1080/01652176.2014.966173

Cited by 9 publications

(4 citation statements)

References 23 publications

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“…found that IFN-γ and IL-4 significantly enhanced specific Th1 and Th2 cell immune responses, respectively ( 33 ). All the above evidence suggests that AMH-INH-RFRP DNA vaccine can induce both humoral and cellular immune response through nasal immunization in buffalo, which is consistent with the previous studies on GPV-VP1 DNA vaccine ( 34 ).…”

Section: Discussionsupporting

confidence: 91%

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Nasal immunization with AMH-INH-RFRP DNA vaccine for improving follicle development and fertility in buffaloes

Chen

Zhao²,

An³

et al. 2023

Front. Endocrinol.

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IntroductionInhibin DNA vaccine has already been proven to improve the fertility of animals. This study aimed to investigate the effects of a novel Anti-Müllerian hormone (AMH)-Inhibin (INH)-RF-amide-related peptides (RFRP) DNA vaccine on immune response and reproductive performance in buffalo.MethodsA total of 84 buffaloes were randomly divided into four groups and nasally immunized twice a day with 10 ml of either AMH-INH-RFRP DNA vaccines (3 × 1010 CFU/ml in group T1, 3 × 109 CFU/ml in group T2, and 3 × 108 CFU/ml in group T3) or PBS (as a control) for 3 days, respectively. All animals received a booster dose at an interval of 14 days.ResultsELISA assay revealed that primary and booster immunization significantly increased the anti-AMH, anti-INH, and anti-RFRP antibody titers in the T2 group compared with that in the T3 group. After the primary immunization, the antibody positive rate was significantly higher in the T2 group than that in the T3 group. In addition, ELISA results indicated that concentrations of E2, IFN-γ, and IL-4 were significantly higher in the antibody-positive (P) group compared to the antibody-negative (N) group. In contrast, there was no significant difference in the concentrations of P4 between the P and N groups. Ultrasonography results revealed a highly significant increase of 2.02 mm in the diameter of ovulatory follicles in the P group compared to the N group. In parallel, growth speed of dominant follicles was significantly higher in the P group than that in the N group (1.33 ± 1.30 vs 1.13 ± 0.12). Furthermore, compared to N group, the rates of oestrus, ovulation, and conception were also significantly higher in the P group.ConclusionThe novel AMH-INH-RFRP DNA vaccine improves the proportion of oestrus, ovulation, and conception in buffalo by promoting the production of E2 and the growth of follicles.

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Section: Discussionsupporting

confidence: 91%

“…All the above evidence suggests that AMH-INH-RFRP DNA vaccine can induce both humoral and cellular immune response through nasal immunization in buffalo, which is consistent with the previous studies on GPV-VP1 DNA vaccine (34).…”

Section: A B D E Csupporting

confidence: 91%

Nasal immunization with AMH-INH-RFRP DNA vaccine for improving follicle development and fertility in buffaloes

Chen

Zhao²,

An³

et al. 2023

Front. Endocrinol.

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“…However, little is known about cellular immunity against GPV. The pcDNA-GPV-VP1 DNA vaccine was shown to induce both cellular and humoral responses, and it can induce higher cellular and humoral immunity than live attenuated vaccine (Deng et al, 2014). The codon-optimized VP2 (optVP2) is capable of forming VLPs, which are highly immunogenic.…”

Section: Discussionmentioning

confidence: 99%

Construction of expression vectors of capsid proteins from goose parvovirus and investigation of the immunogenicity

Tu¹,

Liu²,

Liu³

et al. 2018

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Derzsy's disease is an acute infectious disease caused by GPV that can cause low spirits, loss of feathers, stunted growth, diarrhea and neurological symptoms in geese (Palya et al., 2009). Infected geese have typical conditions such as hydropericardium, ascites, enteritis, and degenerative and inflammatory changes of the myocardium and liver (Jansson et al., 2007). GPV is a small icosahedral virus with a

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“…With an average weight of 8–13 kg at 70–90 days of age, STE are one of the largest goose breeds in the world. WZE are among the smallest goose breeds in the world, normally weighing 2–3 kg at 70–90 days of age [ 1 ]. Although the two goose breeds living in Guangdong province have similar growth cycles, their growth performance, as measured by growth rate and body weight, is quite different.…”

Section: Introductionmentioning

confidence: 99%

DNA Demethylation of Myogenic Genes May Contribute to Embryonic Leg Muscle Development Differences between Wuzong and Shitou Geese

Zhang

Zhu

et al. 2023

IJMS

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Skeletal muscle development from embryonic stages to hatching is critical for poultry muscle growth, during which DNA methylation plays a vital role. However, it is not yet clear how DNA methylation affects early embryonic muscle development between goose breeds of different body size. In this study, whole genome bisulfite sequencing (WGBS) was conducted on leg muscle tissue from Wuzong (WZE) and Shitou (STE) geese on embryonic day 15 (E15), E23, and post-hatch day 1. It was found that at E23, the embryonic leg muscle development of STE was more intense than that of WZE. A negative correlation was found between gene expression and DNA methylation around transcription start sites (TSSs), while a positive correlation was observed in the gene body near TTSs. It was also possible that earlier demethylation of myogenic genes around TSSs contributes to their earlier expression in WZE. Using pyrosequencing to analyze DNA methylation patterns of promoter regions, we also found that earlier demethylation of the MyoD1 promoter in WZE contributed to its earlier expression. This study reveals that DNA demethylation of myogenic genes may contribute to embryonic leg muscle development differences between Wuzong and Shitou geese.

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Evaluation of the immune response in Shitou geese (Anser anser domesticus) following immunization with GPV-VP1 DNA-based and live attenuated vaccines

Abstract: This study showed that it is possible to induce both cellular and humoral response using DNA-based vaccines and that the pcDNA-GPV-VP1 DNA gene vaccine induced better cellular and humoral immunity than live attenuated vaccine.

Cited by 9 publications

References 23 publications

Nasal immunization with AMH-INH-RFRP DNA vaccine for improving follicle development and fertility in buffaloes

Nasal immunization with AMH-INH-RFRP DNA vaccine for improving follicle development and fertility in buffaloes

Construction of expression vectors of capsid proteins from goose parvovirus and investigation of the immunogenicity

DNA Demethylation of Myogenic Genes May Contribute to Embryonic Leg Muscle Development Differences between Wuzong and Shitou Geese

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