Evaluation of the immune responses against reduced doses of Brucella abortus S19 (calfhood) vaccine in water buffaloes (Bubalus bubalis), India

Shome, Rajeswari; Kilari, Sreenivasulu; Sahare, Amol; Kalleshamurthy, Triveni; Niranjanamurthy, Harish Heballi; Shome, Bibek Ranjan; Hiremath, Jagadish; Misri, Jyoti; Rahman, Husna Abdul

doi:10.1016/j.vaccine.2020.09.010

Cited by 8 publications

(5 citation statements)

References 31 publications

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“…Antibody response was high during 21-45 DPV and declined gradually as the number of DPVs increased. Increased Per cent Positivity (PP) values in ELISA after 14 DPV, reaching peak during 28 DPV and declining thereafter at 120 DPV in an experimental S19 vaccination trial conducted in buffalo calves has been similarly reported (Shome et al 2020). In this study, there was a gradual reduction in the number of positives from the group DPV21_45 to DPV181_240 with decreased PP values of <50% in ELISA from DPV61_120.…”

supporting

confidence: 79%

Sero-monitoring of calf hood vaccination under brucellosis control program in selected states of India during 2016-18

Nagalingam

Kalleshamurthy

Skariah

et al. 2022

Indian J of Anim Sci

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The study aimed to evaluate the post-vaccination antibody response in sera of 4-8 months old female calves vaccinated with Brucella S19 vaccine under Brucellosis- Control Program (B-CP) initiated by Department of Animal Husbandry and Dairying (DAH&D), Government of India during 2016-18. The antibody response was extremely good in three states [Telangana (82.53%), Himachal Pradesh (80.97%) and Maharashtra (74.02%)]. Higher antibody response was observed during 21-45 days post-vaccination (DPV) which indicated this period as appropriate for sampling to assess the antibody response. The knowledge acquired with respect to the post-vaccination sero-monitoring in this study will help the ongoing brucellosis control program under the flagship program of Government of India launched in 2019 as National Animal Disease Control Program (NADCP).

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supporting

confidence: 79%

Sero-monitoring of calf hood vaccination under brucellosis control program in selected states of India during 2016-18

Nagalingam

Kalleshamurthy

Skariah

et al. 2022

Indian J of Anim Sci

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“…Two different doses had been identified in Brucella S 19 vaccination in cattle and buffaloes, both high (high log of colony forming unit) and reduced doses (1-3 log reduction of colony forming unit) of Brucella abortus S 19 vaccination are practised globally; a high dose of Brucella abortus S 19 may cause prolonged persistent antibody responses, which interfere with serological screening in cattle and buffaloes in the field (Simpson et al, 2018;Ardiansyah et al, 2019). However, both doses or inoculums (100 times or 2 logs and 1000 times or 3 logs reduction of infective dose) had been shown a more or less similar in immune responses in cattle (Shome et al, 2023;Shome et al, 2020). Since it is a live vaccine, Brucella abortus S 19 multiplies in the natural host (Alton et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

Detectable immunological response in Brucella abortus s 19 vaccinated herds of cattle in vilachchiya veterinary range, north central province, Sri Lanka

Herath,

Fernando,

Weerasooriya

et al. 2023

Sri Lanka Vet. J.

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Brucellosis a zoonotic disease caused by Gram-negative, intracellular, coccobacillus. Brucellosis, an economically important disease in livestock with zoonotic implications has been reported worldwide. It is transmitted via contaminated animals and their products. Clinical signs of animal brucellosis are abortions, reduced fertility, retention of fetal membranes, endometritis, and substantial decline in milk production over an animal’s lifespan. Orchitis, epididymitis and chronic hygroma had been reported in bulls. The current study focused on to studying the detectable immunological responses to Brucella S 19 live attenuated vaccine using serological tests in cattle of dry zone farms. A questionnaire-based survey was carried out in selected farms with and without a previous history of abortion respectively. Animals that were in 1st to 5th lactation in the 18 farms were selected for screening by Rose Bengal Plate Test (RBPT) and positives were confirmed by Complement Fixation Test (CFT). Three farms each with and without history of abortion were selected for Brucella S19 vaccination from the RBPT and CFT negative farms. Sixty non pregnant heifers over 6 months were selected, and 1ml each of S19 vaccine was given subcutaneously to 30 animals while 30 animals were kept as controls. The serum samples were collected and screened for Brucellosis by RBPT and CFT at 2, 4, 6, 8 and 10 weeks of post vaccination. The second week after the vaccination, 71.42% of the animals showed over 1:4 CFT levels while 28.57% of them did not show any antibody titres for Brucellosis in the Brucella endemic farms. In Brucella non-endemic farms, 68.75% of animals showed over 1:4 CFT titre at 2 weeks after the vaccination. However, 31.25% of animals did not show any CFT titre. No persistent and detectable antibody responses were found 10 weeks post vaccination in heifers of endemic and non-endemic herds. The titers of CFT indicate IgG or IgM humeral response, cell mediated immune response of live attenuated vaccine has not been monitored in the study. Thus, seropositivity after the 10 weeks post vaccination may indicate an infection of Brucellosis since no antibodies from vaccination were found. Further investigation with highly sensitive tests such as ELISA is recommended to have a better insight into this aspect in the future.

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“…The results of another study carried out with buffaloes revealed that the immune response and the duration depend on the dose of the S19 vaccine. In addition to this, an S19 vaccine containing 4x10 9 cfu/dose, which is similar to the reduced dose, is recommended to get adequate protection for buffalo calves (Shome et al, 2020).…”

Section: Investigating Field Efficacy and Safety Of Conjunctival Brucella Abortus S19 Vaccine In Cattlementioning

confidence: 99%

“…Prevention of serious epidemic actually depends upon the high level of vaccine efficacy and coverage (Asokan, 2009). It should also be taken into consideration that the effectiveness of a vaccine in the target hosts is diversified by several factors such as feeding, environmental stress, vaccination dose, administration route, immunization and disease prevalence in herds (Schurig et al 2002;Shome et al, 2020).…”

Section: Investigating Field Efficacy and Safety Of Conjunctival Brucella Abortus S19 Vaccine In Cattlementioning

confidence: 99%

Investigating Field Efficacy and Safety of Conjunctival Brucella abortus S19 Vaccine in Cattle

Karagül

Saytekin

Baklan

et al. 2022

ASD

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Background: Vaccination is the most fundamental strategy in the control and eradication of brucellosis. Several vaccination programs with different vaccines have been carried out in many countries in which brucellosis continues to be a problem in livestock. One of the recommended vaccines against brucellosis in cattle is the live Brucella abortus S19 vaccine. The aim of this study is to evaluate the results of field safety and efficacy trials for the conjunctival Brucella abortus S19 vaccine prior to the mass vaccination program. Methods: In this study, 81 female cattle were vaccinated with a reduced dose of Brucella abortus S19 vaccine with the conjunctival route. The immune response after vaccination was investigated by suggested serological tests; namely, Rose Bengal Plate Test, Serum Agglutination Test and Complement Fixation Test. Result: No adverse effect was observed within the scope of safety. Isolation of vaccine strain was observed only in a milk sample of lactating animals. Excluding the diagnosis criteria of the serological tests, humoral immune response was observed in most of the animals by all the serological tests one month after vaccination. Antibody levels lasted approximately 4 months after vaccination. In conclusion, the results of this study demonstrated that besides vaccine-induced antibodies, the vaccine including changes in dose and administration way in this study did not cause any significant risks for the target animals.

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Evaluation of the immune responses against reduced doses of Brucella abortus S19 (calfhood) vaccine in water buffaloes (Bubalus bubalis), India

Cited by 8 publications

References 31 publications

Sero-monitoring of calf hood vaccination under brucellosis control program in selected states of India during 2016-18

Sero-monitoring of calf hood vaccination under brucellosis control program in selected states of India during 2016-18

Detectable immunological response in Brucella abortus s 19 vaccinated herds of cattle in vilachchiya veterinary range, north central province, Sri Lanka

Investigating Field Efficacy and Safety of Conjunctival Brucella abortus S19 Vaccine in Cattle

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