Evaluation of the immunogenicity and protective ability of a pili subunit, SBP2’, of Streptococcus suis serotype 2

Guo, Genglin; Kong, Xuewei; Wang, Zhuohao; Li, Min; Tan, Zhongming; Zhang, Wei

doi:10.1016/j.rvsc.2021.05.006

Cited by 8 publications

(7 citation statements)

References 37 publications

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“…The immune responses generated by PstB immunization were further studied, and high levels of cytokines IFN-γ and IL-4 were observed in the immunized mice. These two cytokines are generally considered indicators for Th1-and Th2type immune responses, respectively, which are important assessment indices of vaccine development for S. suis [15,23,[25][26][27]. Our results suggest that immunization with PstB could stimulate mixed Th1/Th2 responses that resist S. suis infection.…”

Section: Discussionmentioning

confidence: 71%

“…Th1-type and Th2-type immune responses are critical for resistance to S. suis infection [9]. Sera, lung, and spleen samples of immunized mice at 10 days post-booster immunization were collected, and ELISA was used to analyze these samples for the induction level of IFN-γ and IL-4, as indicator cytokines for Th1-type and Th2-type immune responses in S. suis infection, respectively [23][24][25][26][27]. For sera samples, compared with the PBS control group, the immunization of mice with rPstB or rPstB-epitope generated significantly higher levels of both IFN-γ and IL-4.…”

Section: Protection Of Rpstb and Rpstb-epitope Against S Suis Infecti...mentioning

confidence: 99%

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Subunit Vaccine Targeting Phosphate ABC Transporter ATP-Binding Protein, PstB, Provides Cross-Protection against Streptococcus suis Serotype 2, 7, and 9 in Mice

Yan

Yao

Pan

et al. 2023

Veterinary Sciences

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Streptococcus suis is a significant pathogen in pigs and a newly emerging zoonotic agent in humans. The presence of multiple serotypes and strains with diversified sequence types in pig herds highlights the need for the identification of broadly cross-reactive universal vaccine antigen targets, capable of providing cross-protection against S. suis infection. Subunit vaccines based on the conserved proteins shared between different S. suis serotypes are potential candidates for such a universally protective vaccine. In the present study, phosphate ABC transporter ATP-binding protein PstB (PstB), an immunogenic protein of the S. suis bacterium, was expressed and purified, and then subjected to cross-protection evaluation in mice. The PstB protein showed nearly 100% amino acid similarity across a panel of 31 S. suis isolates representing different serotypes, which were collected from different countries. A recombinant PstB (rPstB) protein (S. suis serotype 2) was recognized by rabbit sera specific to this serotype, and induced high levels of IFN-γ and IL-4 in mice immunized with the recombinant protein. These cytokines are considered important for protection against S. suis infection. Immunization of mice with rPstB resulted in an 87.5% protection against challenge with S. suis serotype 2 and 9 strains, suggesting a high level of cross-protection for S. suis serotypes 2 and 9. A lower protection rate (62.5%) was observed in mice challenged with the S. suis serotype 7 strain. These data demonstrate that PstB is a promising target antigen for development as a component of a universal subunit vaccine against multiple S. suis serotypes.

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Section: Discussionmentioning

confidence: 71%

Section: Protection Of Rpstb and Rpstb-epitope Against S Suis Infecti...mentioning

confidence: 99%

Subunit Vaccine Targeting Phosphate ABC Transporter ATP-Binding Protein, PstB, Provides Cross-Protection against Streptococcus suis Serotype 2, 7, and 9 in Mice

Yan

Yao

Pan

et al. 2023

Veterinary Sciences

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“…Thus, the high prevalence of these genes in our invasive isolates can be explained by a relevant role in nutrient acquisition or immune escape activities. Conversely, the S. suis population showed a significant variation in particular VGs such as epf, sly, mrp, ofs, hylA, and sbp2, which were also previously reported as important virulence factors [21,34,58]. Considering their absence in many disease-causing isolates, one may speculate if they are indeed critical for in vivo infection.…”

Section: Discussionmentioning

confidence: 71%

Genomic and phenotypic analysis of invasive Streptococcus suis isolated in Spain reveals genetic diversification and associated virulence traits

Uruén,

Fernandez,

Arnal

et al. 2024

Vet Res

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Streptococcus suis is a zoonotic pathogen that causes a major health problem in the pig production industry worldwide. Spain is one of the largest pig producers in the world. This work aimed to investigate the genetic and phenotypic features of invasive S. suis isolates recovered in Spain. A panel of 156 clinical isolates recovered from 13 Autonomous Communities, representing the major pig producers, were analysed. MLST and serotyping analysis revealed that most isolates (61.6%) were assigned to ST1 (26.3%), ST123 (18.6%), ST29 (9.6%), and ST3 (7.1%). Interestingly, 34 new STs were identified, indicating the emergence of novel genetic lineages. Serotypes 9 (27.6%) and 1 (21.8%) prevailed, followed by serotypes 7 (12.8%) and 2 (12.2%). Analysis of 13 virulence-associated genes showed significant associations between ST, serotype, virulence patterns, and clinical features, evidencing particular virulence traits associated with genetic clusters. The pangenome was generated, and the core genome was distributed in 7 Bayesian groups where each group included a variable set of over- and under-represented genes of different categories. The study provides comprehensive data and knowledge to improve the design of new vaccines, antimicrobial treatments, and bacterial typing approaches.

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“…Furthermore, pili, known as antigens with good immunogenicity, have always been considered good subunit vaccines. Two major pili subunits of srtBCD and srtF , SBP2’ and SFP2, have been shown to have good immunogenicity and can provide immune protection to S. suis in mice model [ 58 , 59 ]. Three different pilus gene clusters were identified in S. parasuis , one of which is homologous with srtF of S. suis , others are different from S. suis , and four different genotypes were found in S. parasuis based on the presence of pili clusters.…”

Section: Discussionmentioning

confidence: 99%

Genomic characterization of Streptococcus parasuis, a close relative of Streptococcus suis and also a potential opportunistic zoonotic pathogen

et al. 2022

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Streptococcus parasuis (S. parasuis) is a close relative of Streptococcus suis (S. suis), composed of former members of S. suis serotypes 20, 22 and 26. S. parasuis could infect pigs and cows, and recently, human infection cases have been reported, making S. parasuis a potential opportunistic zoonotic pathogen. In this study, we analysed the genomic characteristics of S. parasuis, using pan-genome analysis, and compare some phenotypic determinants such as capsular polysaccharide, integrative conjugative elements, CRISPR-Cas system and pili, and predicted the potential virulence genes by associated analysis of the clinical condition of isolated source animals and genotypes. Furthermore, to discuss the relationship with S. suis, we compared these characteristics of S. parasuis with those of S. suis. We found that the characteristics of S. parasuis are similar to those of S. suis, both of them have “open” pan-genome, their antimicrobial resistance gene profiles are similar and a srtF pilus cluster of S. suis was identified in S. parasuis genome. But S. parasuis still have its unique characteristics, two novel pilus clusters are and three different type CRISPR-Cas system were found. Therefore, this study provides novel insights into the interspecific and intraspecific genetic characteristics of S. parasuis, which can be useful for further study of this opportunistic pathogen, such as serotyping, diagnostics, vaccine development, and study of the pathogenesis mechanism.

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Evaluation of the immunogenicity and protective ability of a pili subunit, SBP2’, of Streptococcus suis serotype 2

Cited by 8 publications

References 37 publications

Subunit Vaccine Targeting Phosphate ABC Transporter ATP-Binding Protein, PstB, Provides Cross-Protection against Streptococcus suis Serotype 2, 7, and 9 in Mice

Subunit Vaccine Targeting Phosphate ABC Transporter ATP-Binding Protein, PstB, Provides Cross-Protection against Streptococcus suis Serotype 2, 7, and 9 in Mice

Genomic and phenotypic analysis of invasive Streptococcus suis isolated in Spain reveals genetic diversification and associated virulence traits

Genomic characterization of Streptococcus parasuis, a close relative of Streptococcus suis and also a potential opportunistic zoonotic pathogen

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