Evaluation of the immunogenicity of a recombinant HSV-1 vector expressing human group C rotavirus VP6 protein

Rota, Rosana P.; Palacios, Carlos Adolfo; Temprana, C. Facundo; Argüelles, Marcelo Horacio; Mandile, Marcelo Gastón; Mattion, Nora; Laimbacher, Andrea S.; Fraefel, Cornel; Castello, Alejandro; Glikmann, Graciela

doi:10.1016/j.jviromet.2018.02.020

Cited by 4 publications

(4 citation statements)

References 58 publications

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“…Previous studies from several groups have established that HSV-1 is a safe and effective live vaccine vector, which triggers robust immune responses against multiple infectious pathogens including HIV and rotavirus, among others (45)(46)(47)(48). Herein, we generated a HSV-1-derived vaccine against influenza virus, a significant human and animal respiratory viral pathogen.…”

Section: Discussionmentioning

confidence: 99%

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A Herpes Simplex Virus Type-1-derived influenza vaccine induces balanced adaptive immune responses and protects mice from lethal influenza virus challenge

Rider

Dulin

Uche

et al. 2021

Preprint

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Influenza virus is a major respiratory viral pathogen responsible for the deaths of hundreds of thousands worldwide each year. Current vaccines provide protection primarily by inducing strain-specific antibody responses with the requirement of a match between vaccine strains and circulating strains. It has been suggested that anti-influenza T-cell responses, in addition to antibody responses may provide the broadest protection against different flu strains. Therefore, to address this urgent need, it is desirable to develop a vaccine candidate with an ability to induce balanced adaptive immunity including cell mediated immune responses. A live viral vector technology should exhibit safety, immunogenicity, effectiveness in the presence of pre-existing immunity, and the ability to induce mucosal immune responses. Here, we used VC2, an established Herpes Simplex Virus type 1 vaccine vector, to express the influenza HA protein. We show that this virus is capable of generating potent and specific anti-influenza humoral and cell-mediated immune responses. We further show that a single vaccination with the VC2-derived influenza vaccine protects mice from lethal challenge with influenza virus. Our data support the continued development of VC2-derived influenza vaccines for protection of human populations from both seasonal and pandemic strains of influenza. Finally, our results support the potential of VC2-derived vaccines as a platform for the rapid development of vaccines against emerging and established pathogens, particularly respiratory pathogens.

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Section: Discussionmentioning

confidence: 99%

“…There are a number of groups currently working on HSV-vectored vaccines against a number of human and animal pathogens. Multiple approaches ranging from HSV based amplicons, replication defective, attenuated or conditionally defective vectors have been considered (45)(46)(47)(48).…”

Section: Discussionmentioning

confidence: 99%

A Herpes Simplex Virus Type-1-derived influenza vaccine induces balanced adaptive immune responses and protects mice from lethal influenza virus challenge

Rider

Dulin

Uche

et al. 2021

Preprint

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“…Unlikely RVA, the number of whole RVC genome data is limited. However, over the past few years, there has been an increased interest in human and porcine RVCs, and additional whole genome sequences have been published, which help elucidate their zoonotic relationship [17,33,35,37,[61][62][63][64][65][66][67]. While a clade of VP3 segments of RVC strains from humans shared a common ancestor with porcine strains, Bayesian analysis illustrated these human and porcine strains diverged over a hundred years ago [36].…”

Section: Discussionmentioning

confidence: 99%

Metagenomic sequencing generates the whole genomes of porcine rotavirus A, C, and H from the United States

Hull

Montmayeur

et al. 2020

PLoS ONE

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The genus Rotavirus comprises eight species, designated A to H, and two recently identified tentative species I in dogs and J in bats. Species Rotavirus A, B, C and H (RVA, RVB, RVC and RVH) have been detected in humans and animals. While human and animal RVA are well characterized and defined, complete porcine genome sequences in the GenBank are limited compared to human strains. Here, we used a metagenomic approach to sequence the 11 segments of RVA, RVC and RVH strains from piglets in the United States (US) and explore the evolutionary relations of these RV species. Metagenomics identified Astroviridae, Picornaviridae, Caliciviridae, Coronoviridae in samples MN9.65 and OK5.68 while Picobirnaviridae and Arteriviridae were only identified in sample OK5.68. Whole genome sequencing and phylogenetic analyses identified multiple genotypes with the RVA of strain MN9.65 and OK5.68, with the genome constellation of G5/G9-P[7]/P[13]-I5/I5- R1/R1-C1-M1-A8-N1-T7-E1/E1-H1 and G5/G9-P[6]/P[7]-I5-R1/R1-C1-M1-A8-N1-T1/T7-E1/E1-H1, respectively. The RVA strains had a complex evolutionary relationship with other mammalian strains. The RVC strain OK5.68 had a genome constellation of G9-P[6]-I1-R1-C5-M6-A5-N1-T1-E1-H1, and shared an evolutionary relationship with porcine strains from the US. The RVH strains MN9.65 and OK5.68 had the genome constellation of G5-P1-I1-R1-C1-M1-A5-N1-T1-E4-H1 and G5-P1-I1-R1-C1-M1-A5-N1-T1-E1-H1, indicating multiple RVH genome constellations are circulating in the US. These findings allow us to understand the complexity of the enteric virome, develop improved screening methods for RVC and RVH strains, facilitate expanded rotavirus surveillance in pigs, and increase our understanding of the origin and evolution of rotavirus species.

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“…This recombinant vector also induces the production of neutralizing anti-WNV IgG antibodies in immunized mice [ 148 ]. Cells of BALB/c mice vaccinated with HSV [VP6C] amplicon vector showed the presence of the VP6 protein and specific anti-RVC antibodies against group 6 rotavirus [ 125 ].…”

Section: Dna Virusesmentioning

confidence: 99%

Viruses as tools in gene therapy, vaccine development, and cancer treatment

et al. 2022

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Using viruses to our advantage has been a huge leap for humanity. Their ability to mediate horizontal gene transfer has made them useful tools for gene therapy, vaccine development, and cancer treatment. Adenoviruses, adeno-associated viruses, retroviruses, lentiviruses, alphaviruses, and herpesviruses are a few of the most common candidates for use as therapeutic agents or efficient gene delivery systems. Efforts are being made to improve and perfect viral-vector-based therapies to overcome potential or reported drawbacks. Some preclinical trials of viral vector vaccines have yielded positive results, indicating their potential as prophylactic or therapeutic vaccine candidates. Utilization of the oncolytic activity of viruses is the future of cancer therapy, as patients will then be free from the harmful effects of chemo- or radiotherapy. This review discusses in vitro and in vivo studies showing the brilliant therapeutic potential of viruses.

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Evaluation of the immunogenicity of a recombinant HSV-1 vector expressing human group C rotavirus VP6 protein

Cited by 4 publications

References 58 publications

A Herpes Simplex Virus Type-1-derived influenza vaccine induces balanced adaptive immune responses and protects mice from lethal influenza virus challenge

A Herpes Simplex Virus Type-1-derived influenza vaccine induces balanced adaptive immune responses and protects mice from lethal influenza virus challenge

Metagenomic sequencing generates the whole genomes of porcine rotavirus A, C, and H from the United States

Viruses as tools in gene therapy, vaccine development, and cancer treatment

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