Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats

Abstract

RESUMO: "Avaliação dos efetos imunotoxicológicos de Dimorphandra mollis Benth., Fabaceae, em ratos". A Dimorphandra mollis Benth., Fabaceae, conhecida como faveira ou favad´anta, é uma planta comum do cerrado central do Brasil, muito utilizada por suas propriedades antioxidante, antiplaquetária e, principalmente, como vasoprotetora. Seu principal marcador é a rutina. Este estudo teve como objetivo avaliar a segurança da utilização do extrato seco de D. mollis em roedores. O extrato foi extraído, padronizado e quantificado apresentando teor de 76,0±3% de rutina. Nos parâmetros bioquímicos e hematológicos avaliados, não se observou alterações nos grupos de machos e fêmeas que receberam a dose de 1000 e 2000 mg/kg de D. mollis, mas observou-se um aumento de eosinófilos. Nos estudos histopatológicos detectou-se hiperreatividade da polpa branca esplênica, no grupo que recebeu a dose de 2000 mg/kg de D. mollis. Na avaliação da resposta linfoproliferativa, com 1000 e 2000 mg/kg não foram observadas alterações, e somente nos estudos com a dose de 2000 mg/kg se observou diminuição de IgG. Os resultados obtidos, utilizando roedores, sugerem que nenhuma toxicidade existe na administração de extrato seco de D. mollis na dose de 1000 mg/kg. Unitermos: Dimorphandra mollis, rutina, imunotoxicologia, roedores.ABSTRACT: Dimorphandra mollis Benth., Fabaceae, also known as "faveira" or "fava-d´anta", is a plant common to the central woodsy meadow region of Brazil. It is well known for its antioxidant, antiplatelet and, principally, vasoprotective properties. Its principal component is rutin. The objective of this study is the evaluation of the safety of the use of the dried D. mollis extract in rodents. The rutin content of the standardized extract was 76.0±3%. With respect to the biochemical and hematological parameters evaluated, no alterations in the groups of rats that received 1000 and 2000 mg/kg doses of D. mollis were observed, but an increase in eosinophiles occurred. Hyperactivity of the white splenic pulp was detected in the group that received the 2000 mg/kg dose of D. mollis. In the evaluation of the lymphproliferative response with 1000 and 2000 mg/kg, no alterations were observed, and a decrease in IgG was only observed in the studies with a 2000 mg/kg dose. The results obtained with rodents suggest that no toxicity exists with the administration of dried D. mollis extract in a 1000 mg/kg dose.

Rutin as an Electrochemical Mediator in the Determination of Captopril using a Graphite Paste Electrode

“…The flavonoid rutin (quercetin‐3‐ O ‐rutinoside; Figure B), isolated from Manihot esculenta and Dimorphandra mollis , is used widely by the pharmaceutical industry by virtue of the antioxidant properties associated with its o ‐quinone structure . Although rutin has been reported as a mediator of thiol oxidation in the determination of glutathione using an acetylene black modified carbon paste electrode , to the best of our knowledge, no information is available concerning the application of rutin in the electrochemical oxidation of captopril.…”

Sanitary, physiological quality and alternative treatments of Dimorphandra gardneriana Tul. seeds

“…The fava-d'anta or faveira, as it is known the Dimorphandra gardneriana Tul., (FILIZOLA, 2013) belongs to the Fabaceae family and occurs in the Brazilian states of Maranhão, Piauí, Ceará, Pernambuco, Bahia, Pará, Goiás, Mato Grosso and Minas Gerais (MONTANO et al, 2007). The species is mainly used for medicinal purposes, the fruits contains a large quantity of secondary compounds, among them rutin (quercetin-3rutinosidium), which is a glycoside flavonoid used in popular medicine and generally indicated as a vasoprotectant, and causes an increase in the resistance of blood capillaries (FERES et al, 2010). This compound is widely explored by the pharmaceutical and cosmetic industry and is considered one of the most promising substances for the production of drugs that helps to combat aging and degenerative diseases (GONÇALVES et al, 2010).…”

“…These products consist of an environmentally friendly alternative, less pollutant, easy to be acquired and at a lower cost (BOFF and ALMEIDA, 2005); however, their fungitoxic activity should not cause germination damage. Good results of the use of essential oils on mycoflora reduction were obtained with the use of copaiba oil (Copaifera multijuga Hayne) (DEUS et al, 2011), castor bean oil (Ricinus communis L.) (SORATO et al, 2016), among others.…”