2024
DOI: 10.1101/2024.01.18.24301434
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Evaluation of the impact of concentration and extraction methods on the targeted sequencing of human viruses from wastewater

Minxi Jiang,
Audrey L.W. Wang,
Nicholas A. Be
et al.

Abstract: Sequencing human viruses in wastewater is challenging due to their low abundance compared to the total microbial background. This study compared the impact of four virus concentration/extraction methods (Innovaprep, Nanotrap, Promega, Solids extraction) on probe-capture enrichment for human viruses followed by sequencing. Different concentration/extraction methods yielded distinct virus profiles. Innovaprep ultrafiltration (following solids removal) had the highest sequencing sensitivity and richness, resultin… Show more

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