Evaluation of the impact of a rat small intestinal extract on the digestion of four different functional fibers

Gallego-Lobillo, Pablo; Ferreira-Lazarte, Álvaro; Hernández‐Hernández, Oswaldo; Montilla, Antonia; Villamiel, Mar

doi:10.1039/d0fo00236d

Cited by 12 publications

(4 citation statements)

References 46 publications

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“…Inulin-derived fructans may include FOS without a terminal glucose residue (FF n type) in its composition, which were not found in microbial-FOS [ 20 ]. Recently, inulobiose, inulotriose, inulotetraose, and nystose were identified in a Raftilose ® P95 sample by other authors [ 35 ]. Raftilose ® P95 also showed a higher DP (2–7) than the microbial-FOS, and was mainly composed of tri-, tetra-, and penta-oligomers ( Table S4 ), which is also in accordance with the literature [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Microbial-Fructo-Oligosaccharides Metabolism by Human Gut Microbiota Fermentation as Compared to Commercial Inulin-Derived Oligosaccharides

Roupar

Damasceno

Gonçalves

et al. 2022

Foods

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Section: Discussionmentioning

confidence: 99%

Evaluation of Microbial-Fructo-Oligosaccharides Metabolism by Human Gut Microbiota Fermentation as Compared to Commercial Inulin-Derived Oligosaccharides

Roupar

Damasceno

Gonçalves

et al. 2022

Foods

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“…It is traditionally accepted that prebiotics are not digested and reach the colon unaltered. However, several studies have shown the partial hydrolysis by small intestinal enzymes of potential prebiotics such as isomalto/malto‐polysaccharides, 38 rhamnogalacturonan I 39 and GOS derived from lactose and lactulose 25,40,41 . Differences in the digestibility behavior between di‐ and trisaccharides derived from lactose were observed using digestive enzymes from several sources 40 .…”

Section: Resultsmentioning

confidence: 99%

“…However, several studies have shown the partial hydrolysis by small intestinal enzymes of potential prebiotics such as isomalto/malto-polysaccharides, 38 rhamnogalacturonan I 39 and GOS derived from lactose and lactulose. 25,40,41 Differences in the digestibility behavior between di-and trisaccharides derived from lactose were observed using digestive enzymes from several sources. 40 The glycosidic bonds and the presence of aldoses or ketoses were parameters that influenced the degree of digestibility.…”

Section: In Vitro Digestibilitymentioning

confidence: 99%

In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β‐galactosidase from Lactobacillus delbruecki subsp. bulgaricusCRL450

Fara,

Hernández Hernández,

Palacios

et al. 2024

J Sci Food Agric

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BackgroundTo date, the general belief that prebiotics reach the colon without any alterations is challenged. Some in vitro and in vivo studies have demonstrated that ‘non‐digestible’ oligosaccharides are digested to different degrees depending on their structural composition. In this work, we compared different methods to assess the digestibility of oligosaccharides synthesized by β‐galactosidase (β‐gal) of Lactobacillus delbruecki subsp. bulgaricus CRL450 (CRL450‐β‐gal) from lactose, lactulose and lactitol.ResultsIn simulated gastrointestinal fluid (SGIF) method no changes were observed. However, the oligosaccharides synthesized by CRL450‐β‐gal were partially hydrolyzed in vitro, depending on their structure and composition, with rat small intestinal extract (RSIE) and small intestinal brush‐border membrane vesicles (BBMV) from pig. Digestion of some oligosaccharides increased when mixtures were fed to C57BL/6 mice used as in vivo model, however, lactulose‐oligosaccharides were the most resistant to the physiological conditions of mice. In general β(1 → 6) linked products showed higher resistance compared to β(1 → 3) oligosaccharides.ConclusionIn vitro digestion methods, without disaccharidases, may underestimate the importance of carbohydrates hydrolysis in the small intestine. Although BVMM and RSIE digestion assays are appropriate in vitro methods for these studies, in vivo studies remain the most reliable for understanding what actually happens in the digestion of oligosaccharides.This article is protected by copyright. All rights reserved.

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“…The trehalase-indigestible characteristic of lactulose made it have a higher therapeutic potential than trehalose in vivo. However, lactulose was reported metabolized into fructose, galactose, and glucose in the large intestine. , Recent publications have also pointed out both in vitro and in vivo studies showing the lactulose can be partially digested in the small intestine. − These data reveal the partial digestion of lactulose in the gut could reduce its efficacy in the brain.…”

Section: Discussionmentioning

confidence: 99%

Prebiotic Lactulose Ameliorates the Cognitive Deficit in Alzheimer’s Disease Mouse Model through Macroautophagy and Chaperone-Mediated Autophagy Pathways

Lee

Lai

Huang

et al. 2021

J. Agric. Food Chem.

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Lactulose, as a prebiotic, can be utilized by human gut microbiota and stimulate their growth. Although microbiota modulation has become an emerging approach to manage many diseases and can be achieved by the administration of prebiotics, fewer investigations have been carried out on the therapeutic mechanism of lactulose. Two trehalose analogs, lactulose and melibiose, were identified as having a neuroprotective effect in polyglutamine and Parkinson disease models. In this study, we examined lactulose and melibiose in a mouse primary hippocampal neuronal culture under the toxicity of oligomeric Aβ25–35. Lactulose was further tested in vivo because its effective concentration is lower than that of melibiose. Lactulose and trehalose were applied individually to mice before a bilateral intrahippocampal CA1 injection of oligomeric Aβ25–35. The administration of lactulose and trehalose attenuated the short-term memory and the learning retrieval of Alzheimer’s disease (AD) mice. From a pathological analysis, we found that the pretreatment of lactulose and trehalose decreased neuroinflammation and increased the levels of the autophagic pathways. These results suggest that the neuroprotective effects of both lactulose and trehalose are achieved through anti-inflammation and autophagy. In addition, lactulose was better than trehalose in the enhancement of the synaptic protein expression level in AD mice. Therefore, lactulose could potentially be developed into a preventive and/or therapeutic disaccharide for AD.

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Evaluation of the impact of a rat small intestinal extract on the digestion of four different functional fibers

Abstract: The degree of digestion, modulated by rat small intestinal extract on different functional fibers was investigated.

Cited by 12 publications

References 46 publications

Evaluation of Microbial-Fructo-Oligosaccharides Metabolism by Human Gut Microbiota Fermentation as Compared to Commercial Inulin-Derived Oligosaccharides

Evaluation of Microbial-Fructo-Oligosaccharides Metabolism by Human Gut Microbiota Fermentation as Compared to Commercial Inulin-Derived Oligosaccharides

In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β‐galactosidase from Lactobacillus delbruecki subsp. bulgaricusCRL450

Prebiotic Lactulose Ameliorates the Cognitive Deficit in Alzheimer’s Disease Mouse Model through Macroautophagy and Chaperone-Mediated Autophagy Pathways

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